Fusarium flagelliforme

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina;

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13039 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A395MWI9|A0A395MWI9_9HYPO Uncharacterized protein OS=Fusarium flagelliforme OX=2675880 GN=FIE12Z_3652 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84ISASLGVLVATGLLGHH18 pKa = 6.34SASQATDD25 pKa = 3.23YY26 pKa = 11.54GNNNGSPVGNQPGASGNGIPGNNGGASSSMPYY58 pKa = 9.81PSNAVGSVPGSMPSGVGPVGPVSPGSNGLPPTVPGGIPGNLPGSTSPGDD107 pKa = 3.59ASNGYY112 pKa = 8.52PPNGPNGSPGNFPGPVSPGGGSNSLPPNGPGGVPGSIPGSIPGNSPINGPGNVPGNVPGNAPGNVPGGVPGAGSVIVPGSFPTNIPQNVPGAPGSPDD209 pKa = 3.11SDD211 pKa = 3.19ASNVPTCPLRR221 pKa = 11.84STKK224 pKa = 9.97TVVVTVYY231 pKa = 9.76STGSQNEE238 pKa = 4.27SDD240 pKa = 5.28FPWPDD245 pKa = 3.59DD246 pKa = 3.87SDD248 pKa = 4.67SDD250 pKa = 4.13GSSTPTPMPTSPPGATPWIPTSGIPIFTTLTLDD283 pKa = 2.48IWGPDD288 pKa = 3.49GSPSNSGSDD297 pKa = 3.83SNDD300 pKa = 3.05GTSGNGNNPSGSGSPGDD317 pKa = 3.67GSGGNDD323 pKa = 3.51GANSGSGNGSDD334 pKa = 4.81SDD336 pKa = 5.15GDD338 pKa = 3.88DD339 pKa = 3.5SQNNPGSPFPFPASGPNGAPQYY361 pKa = 10.25SDD363 pKa = 3.02NRR365 pKa = 11.84FGGSSAGIPGATGDD379 pKa = 3.56NTYY382 pKa = 10.68VHH384 pKa = 6.9TPLQTPVSPNGGVTGNQPGLPSTGPAQEE412 pKa = 4.95ASDD415 pKa = 4.18SLPVTTQAGPEE426 pKa = 4.25GQGEE430 pKa = 4.17NSPWLTNVPGGNGASQPSPSYY451 pKa = 9.91ITITGQDD458 pKa = 3.7GLPTVVSNGGNMIPGQAQPSANSGFPPNGNNPGLPVGGSSGFPLPPPAITGTPGSQNPSNSPAGSLPGSGPDD530 pKa = 4.2EE531 pKa = 5.46GITTCATFTITGTDD545 pKa = 3.65GLPTVVDD552 pKa = 4.1TTWVIPGTFDD562 pKa = 3.25PQAPLSTITSEE573 pKa = 4.36LGLPTFPFTIPGSPGDD589 pKa = 4.0ASGHH593 pKa = 5.17SAMTTSTSYY602 pKa = 11.2TMIGADD608 pKa = 3.6GNPTVVHH615 pKa = 5.2TTWTIPTAEE624 pKa = 4.81AGFGDD629 pKa = 4.63PGDD632 pKa = 3.82KK633 pKa = 10.42SALVSSGDD641 pKa = 3.4FMTVTEE647 pKa = 5.04LPPFGSSGSGGDD659 pKa = 3.74NPTAPNSDD667 pKa = 3.39NASPITTCTSYY678 pKa = 10.59TVIGTDD684 pKa = 3.59GLPTVVDD691 pKa = 3.73STFVIPGPVNTGATAGGDD709 pKa = 3.52PQVPSGASDD718 pKa = 3.82ALPNGATSPATIQITSSPGLTPPAPTDD745 pKa = 3.35GTGIGSIGNGDD756 pKa = 3.06ITTCTSYY763 pKa = 10.86TITGPDD769 pKa = 3.89GLPTIVDD776 pKa = 4.18STWVIPGPANTQSEE790 pKa = 4.82LPGNPSFVSDD800 pKa = 5.03SIPSGLPTGFPGPMTASAGIPSDD823 pKa = 4.53DD824 pKa = 4.07NQGNMPGVTTCITYY838 pKa = 7.52TTIGSDD844 pKa = 3.66GLPTVVDD851 pKa = 3.73TTFVIPVATATPAGTSLNVNGNGQTGSSPNPTGAFTTFTTAIVLGPDD898 pKa = 3.76GNPTSTVQTIVFSDD912 pKa = 3.83SSALGVSAGPPQGATSGVTPLGPSGPVFTTGDD944 pKa = 4.03SNSLPTDD951 pKa = 3.6TPSLNGYY958 pKa = 10.3DD959 pKa = 4.19DD960 pKa = 5.97GIPGASISVVLTGGSAAAQGTSVSGTTTGTLTWTVTSITNPAGGPVFSQGAGQPPFASGPSDD1022 pKa = 3.27NSASDD1027 pKa = 4.01GMAQPAYY1034 pKa = 10.14GSVATEE1040 pKa = 3.8STLFPLSAIQTSTWTNVIKK1059 pKa = 10.92AEE1061 pKa = 4.24TTSYY1065 pKa = 9.33TFNYY1069 pKa = 9.4PVTTLATVNVPKK1081 pKa = 10.56KK1082 pKa = 10.13RR1083 pKa = 11.84FARR1086 pKa = 11.84RR1087 pKa = 11.84QEE1089 pKa = 3.54MTAWSNSTTSASSAISASTSTTSEE1113 pKa = 3.72ASSPSICPAGGNIGNTTIDD1132 pKa = 3.86FDD1134 pKa = 4.06TSKK1137 pKa = 10.53PGPLFNPVEE1146 pKa = 4.53NIWFSGGFLIAPPATQQSQPYY1167 pKa = 8.49IPSSGGQLVEE1177 pKa = 4.92FVPPALSNTTTTISGDD1193 pKa = 3.29VAQIGVGPHH1202 pKa = 6.14AASPCFRR1209 pKa = 11.84FDD1211 pKa = 3.51FFGAEE1216 pKa = 4.46LGCDD1220 pKa = 3.36AQGDD1224 pKa = 4.26EE1225 pKa = 4.8EE1226 pKa = 4.43WCEE1229 pKa = 4.02FDD1231 pKa = 3.62ISAYY1235 pKa = 9.59RR1236 pKa = 11.84WNEE1239 pKa = 3.59TSSTEE1244 pKa = 4.22EE1245 pKa = 3.76SIAWSEE1251 pKa = 4.39TKK1253 pKa = 9.86QVPACPKK1260 pKa = 9.5FSEE1263 pKa = 4.6GGYY1266 pKa = 9.82QLTRR1270 pKa = 11.84VDD1272 pKa = 3.71LDD1274 pKa = 3.79GYY1276 pKa = 11.0KK1277 pKa = 10.39DD1278 pKa = 3.66LSSILITLRR1287 pKa = 11.84VSSNLRR1293 pKa = 11.84VWWGDD1298 pKa = 3.26DD1299 pKa = 3.78FRR1301 pKa = 11.84VGWTDD1306 pKa = 3.2NSCVAASCRR1315 pKa = 11.84ANAPSQFVKK1324 pKa = 10.72RR1325 pKa = 11.84EE1326 pKa = 4.09TIISALRR1333 pKa = 11.84QGVYY1337 pKa = 7.64QWTPYY1342 pKa = 9.18EE1343 pKa = 4.43LKK1345 pKa = 10.68RR1346 pKa = 11.84LDD1348 pKa = 5.16DD1349 pKa = 3.85SLVWEE1354 pKa = 4.61SANN1357 pKa = 3.76

Molecular weight: 134.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A395N215|A0A395N215_9HYPO Vacuolar calcium ion transporter OS=Fusarium flagelliforme OX=2675880 GN=FIE12Z_1651 PE=3 SV=1
VV1 pKa = 6.97KK2 pKa = 9.91PRR4 pKa = 11.84PAQSGPRR11 pKa = 11.84PGPPVKK17 pKa = 10.33GGPPRR22 pKa = 11.84GPGPMSPNGGRR33 pKa = 11.84PQSPMGPPRR42 pKa = 11.84GPPGHH47 pKa = 6.63PQGSPRR53 pKa = 11.84GQSPGPFQGPPQGRR67 pKa = 11.84SMSPGPRR74 pKa = 11.84SQSPGPRR81 pKa = 11.84NGPPGGRR88 pKa = 11.84PMSPGPRR95 pKa = 11.84SQSPGPAGGRR105 pKa = 11.84SQSPNGVNRR114 pKa = 11.84RR115 pKa = 11.84NNPPGPSPMNPSQGPTSSGSVNRR138 pKa = 11.84KK139 pKa = 7.96PVPGQAYY146 pKa = 9.86

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13039

0

13039

6531987

28

13355

501.0

55.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.32 ± 0.019

1.268 ± 0.01

5.945 ± 0.016

6.353 ± 0.024

3.757 ± 0.013

6.746 ± 0.022

2.355 ± 0.011

5.061 ± 0.015

5.124 ± 0.019

8.662 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.008

3.837 ± 0.012

5.933 ± 0.025

4.023 ± 0.014

5.783 ± 0.019

8.034 ± 0.022

6.101 ± 0.028

6.094 ± 0.018

1.538 ± 0.009

2.818 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski