Klebsiella phage KP32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; Klebsiella virus KP32

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1L2W5|D1L2W5_9CAUD Tail tubular protein B OS=Klebsiella phage KP32 OX=674082 GN=32 PE=4 SV=1
MM1 pKa = 7.38NMQDD5 pKa = 4.11AYY7 pKa = 10.62FGSAAEE13 pKa = 3.97LDD15 pKa = 4.2AVNEE19 pKa = 3.86MLAAIGEE26 pKa = 4.7SPVTTLDD33 pKa = 3.33EE34 pKa = 5.18DD35 pKa = 4.25GSADD39 pKa = 3.27VANARR44 pKa = 11.84RR45 pKa = 11.84ILNRR49 pKa = 11.84INRR52 pKa = 11.84QIQSKK57 pKa = 9.61GWAFNINEE65 pKa = 4.55SATLTPDD72 pKa = 2.76VSTGLIPFRR81 pKa = 11.84PAYY84 pKa = 10.44LSILGGQYY92 pKa = 10.17VNRR95 pKa = 11.84GGWVYY100 pKa = 11.2DD101 pKa = 3.54KK102 pKa = 11.07STGTDD107 pKa = 3.14TFSGPITVTLITLQDD122 pKa = 3.68YY123 pKa = 11.66DD124 pKa = 4.32EE125 pKa = 4.72MPEE128 pKa = 5.08CFRR131 pKa = 11.84QWIVTKK137 pKa = 10.66ASRR140 pKa = 11.84QFNSRR145 pKa = 11.84FFGAEE150 pKa = 3.73DD151 pKa = 3.76VEE153 pKa = 4.46NSLAQEE159 pKa = 3.88EE160 pKa = 4.75MEE162 pKa = 5.66ARR164 pKa = 11.84MACNEE169 pKa = 3.84YY170 pKa = 10.91EE171 pKa = 4.14MDD173 pKa = 3.84FGQYY177 pKa = 11.01NMLDD181 pKa = 3.06GDD183 pKa = 4.58AYY185 pKa = 10.18VQGLIGRR192 pKa = 4.48

Molecular weight: 21.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1L2X5|D1L2X5_9CAUD Phage lambda Rz-like lysis protein OS=Klebsiella phage KP32 OX=674082 GN=42 PE=4 SV=1
MM1 pKa = 7.54TISSPYY7 pKa = 10.3SDD9 pKa = 3.13ILKK12 pKa = 9.78VLLYY16 pKa = 9.38VVPLWMSLHH25 pKa = 5.25YY26 pKa = 10.53ARR28 pKa = 11.84RR29 pKa = 11.84SYY31 pKa = 11.06VKK33 pKa = 10.3SEE35 pKa = 3.66KK36 pKa = 10.35HH37 pKa = 6.58LEE39 pKa = 4.01ALCWKK44 pKa = 9.19LQRR47 pKa = 11.84EE48 pKa = 4.32SSHH51 pKa = 6.89ILWLSWCSPSGGTWVHH67 pKa = 6.39NLRR70 pKa = 11.84TPNGRR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 10.33YY78 pKa = 10.66RR79 pKa = 11.84MSTLRR84 pKa = 11.84SKK86 pKa = 10.79RR87 pKa = 11.84LEE89 pKa = 3.91LKK91 pKa = 9.87LRR93 pKa = 11.84KK94 pKa = 9.48RR95 pKa = 11.84LTQYY99 pKa = 11.07RR100 pKa = 11.84LSTKK104 pKa = 8.29QTLRR108 pKa = 11.84GWRR111 pKa = 11.84AALIGLLLICVATISGCASKK131 pKa = 11.24SNLPVSPQDD140 pKa = 3.42QTVDD144 pKa = 3.14ASLMVSSNYY153 pKa = 8.82TKK155 pKa = 10.58QLLEE159 pKa = 4.16VLSQQ163 pKa = 3.24

Molecular weight: 18.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

12758

65

1321

290.0

32.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.896 ± 0.476

1.003 ± 0.181

6.208 ± 0.22

6.545 ± 0.421

3.715 ± 0.209

8.011 ± 0.399

1.724 ± 0.18

4.977 ± 0.179

6.537 ± 0.374

8.128 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.154

4.358 ± 0.309

3.762 ± 0.167

4.264 ± 0.326

5.636 ± 0.243

6.153 ± 0.325

5.871 ± 0.304

6.811 ± 0.363

1.458 ± 0.181

3.347 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski