Sphaerochaeta pleomorpha (strain ATCC BAA-1885 / DSM 22778 / Grapes)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Sphaerochaetaceae; Sphaerochaeta; Sphaerochaeta pleomorpha

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8QTW7|G8QTW7_SPHPG Adenylosuccinate synthetase OS=Sphaerochaeta pleomorpha (strain ATCC BAA-1885 / DSM 22778 / Grapes) OX=158190 GN=purA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.4KK3 pKa = 10.27KK4 pKa = 9.79IALVMIVVMMVLFFTGCEE22 pKa = 3.72QQIPITEE29 pKa = 3.99ILSTVKK35 pKa = 10.57GYY37 pKa = 10.19SEE39 pKa = 5.47EE40 pKa = 3.92ILAMVASEE48 pKa = 4.8VEE50 pKa = 4.1TSLPDD55 pKa = 3.53SFTDD59 pKa = 3.52DD60 pKa = 3.21TLIAIDD66 pKa = 3.74TGISCRR72 pKa = 11.84VSNTYY77 pKa = 9.48TNEE80 pKa = 4.44LILDD84 pKa = 3.52ITLNNWVASDD94 pKa = 3.63GTVISGSIEE103 pKa = 3.6MDD105 pKa = 3.67LVYY108 pKa = 10.76DD109 pKa = 4.11ADD111 pKa = 3.53STYY114 pKa = 10.29IYY116 pKa = 9.88SIQSIVLMNYY126 pKa = 10.39DD127 pKa = 3.47LTSVYY132 pKa = 10.36FGEE135 pKa = 4.42EE136 pKa = 4.1VFDD139 pKa = 3.96NTPEE143 pKa = 3.85TAAFTFDD150 pKa = 3.46NATFQCLALSVDD162 pKa = 4.25GKK164 pKa = 10.03PLISNQLWLSLLTRR178 pKa = 11.84KK179 pKa = 9.53

Molecular weight: 19.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8QQ36|G8QQ36_SPHPG Formyltetrahydrofolate deformylase OS=Sphaerochaeta pleomorpha (strain ATCC BAA-1885 / DSM 22778 / Grapes) OX=158190 GN=purU PE=3 SV=1
MM1 pKa = 6.35QTNTSARR8 pKa = 11.84RR9 pKa = 11.84GLLPALFIIPLLFFGLGFISILSALSAFVCMALPFILLAKK49 pKa = 9.87NKK51 pKa = 10.0RR52 pKa = 11.84KK53 pKa = 7.31TWCHH57 pKa = 5.94TYY59 pKa = 10.36CPRR62 pKa = 11.84ASLLQQTGKK71 pKa = 10.42KK72 pKa = 8.43GNKK75 pKa = 7.98MKK77 pKa = 10.35QAPRR81 pKa = 11.84QFTDD85 pKa = 3.65GSLRR89 pKa = 11.84KK90 pKa = 9.37ILLWYY95 pKa = 10.36FGLNLLFITGSTVRR109 pKa = 11.84IALGQMEE116 pKa = 4.82SMAFIRR122 pKa = 11.84LFIFLPLFPLPQLLDD137 pKa = 3.12IAAPPFLIHH146 pKa = 6.86LSYY149 pKa = 11.08RR150 pKa = 11.84LYY152 pKa = 11.77SMMLSSTILGVAFALIWRR170 pKa = 11.84NRR172 pKa = 11.84LWCAVCPVGNLSNKK186 pKa = 9.65ILGIKK191 pKa = 8.99NN192 pKa = 3.24

Molecular weight: 21.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3150

0

3150

1068925

29

2489

339.3

37.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.632 ± 0.047

1.388 ± 0.019

5.124 ± 0.032

6.162 ± 0.041

4.817 ± 0.03

7.195 ± 0.041

1.787 ± 0.018

7.125 ± 0.037

6.043 ± 0.03

10.465 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.02

3.972 ± 0.025

3.83 ± 0.023

3.467 ± 0.024

4.184 ± 0.029

7.074 ± 0.037

5.566 ± 0.034

6.871 ± 0.036

1.021 ± 0.015

3.508 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski