Sandfly fever Turkey virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Sicilian phlebovirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9YRN1|D9YRN1_9VIRU Polyprotein OS=Sandfly fever Turkey virus OX=688699 PE=4 SV=2
MM1 pKa = 7.61INNNMMNSQYY11 pKa = 10.34MFDD14 pKa = 3.88YY15 pKa = 10.01PAINIDD21 pKa = 3.83VRR23 pKa = 11.84CHH25 pKa = 6.41RR26 pKa = 11.84LLSSVSYY33 pKa = 9.13VAHH36 pKa = 6.45NKK38 pKa = 8.98FHH40 pKa = 5.86THH42 pKa = 6.73DD43 pKa = 3.32VSTYY47 pKa = 6.46EE48 pKa = 3.74HH49 pKa = 7.12CEE51 pKa = 3.64IPLEE55 pKa = 3.94KK56 pKa = 10.45LRR58 pKa = 11.84LGFGRR63 pKa = 11.84RR64 pKa = 11.84SSLSDD69 pKa = 3.92FYY71 pKa = 11.94SLGEE75 pKa = 4.57LPASWGPACYY85 pKa = 9.56ISSVKK90 pKa = 10.28PMVYY94 pKa = 9.27TFQGMASDD102 pKa = 4.59LSRR105 pKa = 11.84FDD107 pKa = 3.27LTSFSRR113 pKa = 11.84RR114 pKa = 11.84GLPNVLKK121 pKa = 10.71ALSWPLGIPDD131 pKa = 4.57CEE133 pKa = 4.11IFSICSDD140 pKa = 3.33RR141 pKa = 11.84FVRR144 pKa = 11.84GLQTRR149 pKa = 11.84DD150 pKa = 3.11QLMSYY155 pKa = 9.35ILRR158 pKa = 11.84MGDD161 pKa = 3.14SHH163 pKa = 8.69SLDD166 pKa = 3.4EE167 pKa = 5.98CIVQAHH173 pKa = 6.17KK174 pKa = 10.77KK175 pKa = 9.21ILQEE179 pKa = 3.78ARR181 pKa = 11.84RR182 pKa = 11.84LGLSDD187 pKa = 3.2EE188 pKa = 4.92HH189 pKa = 8.04YY190 pKa = 10.95NGYY193 pKa = 10.46DD194 pKa = 3.3LFRR197 pKa = 11.84EE198 pKa = 4.24IGSLVCLRR206 pKa = 11.84LINAEE211 pKa = 4.02PFDD214 pKa = 3.85TAFSGEE220 pKa = 4.08ALDD223 pKa = 3.34IRR225 pKa = 11.84TVIRR229 pKa = 11.84SYY231 pKa = 10.64RR232 pKa = 11.84ASDD235 pKa = 3.55PSASLTEE242 pKa = 4.23YY243 pKa = 11.08GNSLWTPIHH252 pKa = 5.65SHH254 pKa = 5.23VDD256 pKa = 3.44EE257 pKa = 4.83NDD259 pKa = 3.34EE260 pKa = 4.33SSSDD264 pKa = 3.37SDD266 pKa = 3.73FF267 pKa = 4.55

Molecular weight: 30.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4Y5R8|F4Y5R8_9VIRU Nucleoprotein OS=Sandfly fever Turkey virus OX=688699 GN=N PE=3 SV=1
MM1 pKa = 8.37DD2 pKa = 5.38EE3 pKa = 4.07YY4 pKa = 11.49QKK6 pKa = 10.77IAVEE10 pKa = 4.37FGEE13 pKa = 4.12QAIDD17 pKa = 3.36EE18 pKa = 4.57AVIQDD23 pKa = 3.65WLQAFAYY30 pKa = 9.94QGFDD34 pKa = 3.03ARR36 pKa = 11.84TIIQNLVQLGGKK48 pKa = 8.02SWEE51 pKa = 3.93EE52 pKa = 3.77DD53 pKa = 3.16AKK55 pKa = 11.55KK56 pKa = 10.12MIILSLTRR64 pKa = 11.84GNKK67 pKa = 7.17PKK69 pKa = 10.89KK70 pKa = 8.29MVEE73 pKa = 3.87RR74 pKa = 11.84MSPEE78 pKa = 3.47GARR81 pKa = 11.84EE82 pKa = 4.1VKK84 pKa = 10.71SLVAKK89 pKa = 9.55YY90 pKa = 10.07KK91 pKa = 10.21IVEE94 pKa = 4.11GRR96 pKa = 11.84PGRR99 pKa = 11.84NGITLSRR106 pKa = 11.84VAAALAGWTVQAVEE120 pKa = 4.28VVEE123 pKa = 4.53NFLPVPGSTMDD134 pKa = 3.67RR135 pKa = 11.84MCGQTYY141 pKa = 8.66PRR143 pKa = 11.84QMMHH147 pKa = 6.99PSFAGLIDD155 pKa = 4.23PSLDD159 pKa = 3.36QEE161 pKa = 4.69DD162 pKa = 4.53FNAVLDD168 pKa = 3.77AHH170 pKa = 7.1KK171 pKa = 10.72LFLLMFSKK179 pKa = 9.88TINVSLRR186 pKa = 11.84GAQKK190 pKa = 10.49RR191 pKa = 11.84DD192 pKa = 3.17IEE194 pKa = 4.4EE195 pKa = 4.49SFSQPMLAAINSSFIDD211 pKa = 3.33NTQRR215 pKa = 11.84RR216 pKa = 11.84AFLTKK221 pKa = 10.63FGILTSGARR230 pKa = 11.84ATAVVKK236 pKa = 10.6KK237 pKa = 10.18IAEE240 pKa = 4.34AYY242 pKa = 8.93RR243 pKa = 11.84KK244 pKa = 10.29LEE246 pKa = 3.91

Molecular weight: 27.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3944

246

2090

986.0

111.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.882 ± 0.796

2.637 ± 0.789

5.806 ± 0.478

6.846 ± 0.231

4.615 ± 0.396

5.958 ± 0.106

2.561 ± 0.231

6.567 ± 0.125

6.237 ± 0.444

8.469 ± 0.379

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.22 ± 0.139

3.829 ± 0.121

3.448 ± 0.118

3.195 ± 0.383

5.958 ± 0.343

8.824 ± 0.49

5.325 ± 0.312

6.694 ± 0.371

1.141 ± 0.114

2.789 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski