Aureibacillus halotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Aureibacillus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6TTI9|A0A4R6TTI9_9BACI ArsR family transcriptional regulator OS=Aureibacillus halotolerans OX=1508390 GN=EV213_11775 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.13QRR4 pKa = 11.84GWIMVIVLAIVVVGFIAIVVLNNQSNGSNANGGSGMYY41 pKa = 9.09TGQGDD46 pKa = 3.93PEE48 pKa = 4.46AFAEE52 pKa = 4.93LYY54 pKa = 10.62SEE56 pKa = 4.26QPVLGDD62 pKa = 3.28EE63 pKa = 4.26NAPVQIVQFGDD74 pKa = 3.91YY75 pKa = 10.31KK76 pKa = 11.25CPACKK81 pKa = 10.21NFEE84 pKa = 4.15EE85 pKa = 4.27QLVPIIKK92 pKa = 9.77QEE94 pKa = 3.95YY95 pKa = 8.36VDD97 pKa = 3.39TGKK100 pKa = 10.68AQFYY104 pKa = 9.59FMNYY108 pKa = 9.19PFLGDD113 pKa = 3.77DD114 pKa = 3.65AAILASYY121 pKa = 10.75AEE123 pKa = 4.32AVYY126 pKa = 8.67ATLGNDD132 pKa = 4.12AFWTYY137 pKa = 11.16NSAVYY142 pKa = 9.61EE143 pKa = 4.18RR144 pKa = 11.84QGDD147 pKa = 3.68EE148 pKa = 3.73RR149 pKa = 11.84LAWGNDD155 pKa = 3.1EE156 pKa = 5.02FNQTLIQEE164 pKa = 4.63LFSEE168 pKa = 4.78EE169 pKa = 4.24EE170 pKa = 3.83ATQVLEE176 pKa = 4.6AYY178 pKa = 9.66EE179 pKa = 4.59SGDD182 pKa = 3.37FADD185 pKa = 4.47IVADD189 pKa = 4.18EE190 pKa = 4.35KK191 pKa = 11.6AFAEE195 pKa = 4.43SQGVTGTPSLFINGKK210 pKa = 9.02KK211 pKa = 10.01VEE213 pKa = 4.21MDD215 pKa = 3.31NLEE218 pKa = 4.12QVADD222 pKa = 4.04LNKK225 pKa = 10.3LVEE228 pKa = 4.3EE229 pKa = 4.55AATNGG234 pKa = 3.44

Molecular weight: 25.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6U5G4|A0A4R6U5G4_9BACI L-rhamnose isomerase/sugar isomerase OS=Aureibacillus halotolerans OX=1508390 GN=EV213_11012 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.14RR3 pKa = 11.84RR4 pKa = 11.84AEE6 pKa = 4.07HH7 pKa = 6.01PQTGKK12 pKa = 9.8WFFHH16 pKa = 6.33FSRR19 pKa = 11.84LEE21 pKa = 3.49ISVPQFQRR29 pKa = 11.84AAAGQRR35 pKa = 11.84KK36 pKa = 9.27AEE38 pKa = 4.11RR39 pKa = 11.84RR40 pKa = 11.84CTEE43 pKa = 3.64AQVRR47 pKa = 11.84TSSGRR52 pKa = 11.84EE53 pKa = 3.73MVLHH57 pKa = 6.67ISLARR62 pKa = 11.84GFLRR66 pKa = 11.84RR67 pKa = 11.84SFYY70 pKa = 10.8GAQLAKK76 pKa = 10.62KK77 pKa = 9.86SGAPCASIRR86 pKa = 11.84IIPKK90 pKa = 9.04PRR92 pKa = 11.84NGSPISRR99 pKa = 11.84FGDD102 pKa = 3.3VFCIKK107 pKa = 9.69EE108 pKa = 4.15PCHH111 pKa = 6.31IPCSLDD117 pKa = 3.25NASPQPYY124 pKa = 9.85LHH126 pKa = 7.2LILNHH131 pKa = 5.73FRR133 pKa = 11.84TFEE136 pKa = 3.96WSSATQEE143 pKa = 4.24SQALMTLYY151 pKa = 10.81SKK153 pKa = 10.93VEE155 pKa = 3.77PEE157 pKa = 3.68RR158 pKa = 11.84RR159 pKa = 11.84PFF161 pKa = 3.67

Molecular weight: 18.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4165

0

4165

1211138

25

2337

290.8

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.956 ± 0.038

0.754 ± 0.011

5.342 ± 0.036

7.244 ± 0.047

4.401 ± 0.027

6.829 ± 0.032

2.387 ± 0.022

6.53 ± 0.035

5.48 ± 0.029

9.969 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.019

3.746 ± 0.025

3.938 ± 0.04

4.207 ± 0.027

4.483 ± 0.028

6.254 ± 0.029

5.865 ± 0.032

7.292 ± 0.047

1.233 ± 0.017

3.265 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski