Beet virus Q

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Pomovirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9WKC1|Q9WKC1_9VIRU Capsid protein OS=Beet virus Q OX=71972 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.48PPVIEE7 pKa = 5.08HH8 pKa = 6.73SQDD11 pKa = 3.43CCCQHH16 pKa = 6.94CSWQPPCTPTCAGLNRR32 pKa = 11.84NAMSHH37 pKa = 4.96VEE39 pKa = 4.17EE40 pKa = 3.89IVRR43 pKa = 11.84RR44 pKa = 11.84EE45 pKa = 3.73EE46 pKa = 4.16SSFSLSYY53 pKa = 10.89VALCCVVCLLLGVTFSLYY71 pKa = 10.37LKK73 pKa = 10.58SGAEE77 pKa = 4.02VDD79 pKa = 3.89SSAFSYY85 pKa = 10.69YY86 pKa = 10.4YY87 pKa = 10.3QDD89 pKa = 3.83LNSVEE94 pKa = 4.43VKK96 pKa = 10.18IGSYY100 pKa = 10.4PIDD103 pKa = 3.75PEE105 pKa = 4.12IIKK108 pKa = 10.35AIHH111 pKa = 6.47HH112 pKa = 5.52FQEE115 pKa = 4.49APFGVSLSQSDD126 pKa = 4.2DD127 pKa = 3.65SDD129 pKa = 4.01VDD131 pKa = 3.85DD132 pKa = 4.89VPDD135 pKa = 3.57VAEE138 pKa = 4.81LALQIDD144 pKa = 5.02RR145 pKa = 11.84LTLSCVVFEE154 pKa = 5.48FIEE157 pKa = 4.2KK158 pKa = 9.12LCYY161 pKa = 10.07RR162 pKa = 11.84FFCVCLVVFCFYY174 pKa = 10.78CYY176 pKa = 10.41FHH178 pKa = 7.17FCC180 pKa = 4.65

Molecular weight: 20.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O92518|O92518_9VIRU Third triple gene block protein OS=Beet virus Q OX=71972 PE=4 SV=1
MM1 pKa = 6.96VRR3 pKa = 11.84SNVVGARR10 pKa = 11.84PNIYY14 pKa = 8.63WPIVVGVVAIALFGFLTITNQKK36 pKa = 10.31HH37 pKa = 4.31STQSGDD43 pKa = 3.87NIHH46 pKa = 6.78KK47 pKa = 9.44FANGGSYY54 pKa = 11.29ADD56 pKa = 3.41GSKK59 pKa = 10.5RR60 pKa = 11.84INYY63 pKa = 7.88NKK65 pKa = 10.16NNCRR69 pKa = 11.84AYY71 pKa = 10.64NGSSSNRR78 pKa = 11.84TFTGLLLPALFLAAALYY95 pKa = 10.29AYY97 pKa = 9.41VCWSKK102 pKa = 10.52PKK104 pKa = 10.51CHH106 pKa = 4.95VTCRR110 pKa = 11.84GDD112 pKa = 3.38CAAGGEE118 pKa = 4.28

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4809

81

1818

534.3

60.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.529 ± 0.864

2.475 ± 0.512

6.717 ± 0.63

7.278 ± 0.562

4.367 ± 0.335

6.114 ± 0.454

2.412 ± 0.159

4.866 ± 0.256

6.821 ± 0.696

8.921 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.539

3.847 ± 0.334

3.098 ± 0.213

2.932 ± 0.17

5.906 ± 0.411

7.632 ± 1.104

5.407 ± 0.231

7.673 ± 0.216

0.998 ± 0.154

3.327 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski