Candidatus Riesia sp. GBBU

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Candidatus Riesia; unclassified Candidatus Riesia

Average proteome isoelectric point is 8.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 402 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0HQ28|A0A1V0HQ28_9ENTR 50S ribosomal protein L10 OS=Candidatus Riesia sp. GBBU OX=1719125 GN=AOQ88_01475 PE=3 SV=1
MM1 pKa = 6.92ITGAAQMDD9 pKa = 4.35GAILVADD16 pKa = 4.09ATDD19 pKa = 3.82GPMPQTRR26 pKa = 11.84EE27 pKa = 4.11HH28 pKa = 6.65ILLARR33 pKa = 11.84QVGVPYY39 pKa = 10.35IVVFLNKK46 pKa = 9.71CDD48 pKa = 3.69MVDD51 pKa = 3.75DD52 pKa = 4.61KK53 pKa = 11.58EE54 pKa = 4.27LLEE57 pKa = 4.11LVEE60 pKa = 4.04MEE62 pKa = 4.36IRR64 pKa = 11.84EE65 pKa = 4.23LL66 pKa = 3.78

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0HPJ9|A0A1V0HPJ9_9ENTR tRNA modification GTPase MnmE OS=Candidatus Riesia sp. GBBU OX=1719125 GN=mnmE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.29RR14 pKa = 11.84SHH16 pKa = 6.08GFRR19 pKa = 11.84LRR21 pKa = 11.84MSRR24 pKa = 11.84KK25 pKa = 8.56SGRR28 pKa = 11.84KK29 pKa = 7.91IISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.89GRR39 pKa = 11.84LSLTVSLRR47 pKa = 11.84RR48 pKa = 11.84GG49 pKa = 3.17

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

402

0

402

129329

38

1402

321.7

37.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.159 ± 0.099

1.28 ± 0.041

4.04 ± 0.07

5.648 ± 0.114

5.809 ± 0.124

5.326 ± 0.108

1.696 ± 0.038

12.222 ± 0.131

11.86 ± 0.165

9.03 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.048

7.008 ± 0.09

2.617 ± 0.059

2.218 ± 0.057

4.223 ± 0.086

8.005 ± 0.102

4.041 ± 0.061

5.205 ± 0.07

0.803 ± 0.036

3.724 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski