Escherichia phage vB_EcoS_HdSG1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Nonagvirus; unclassified Nonagvirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482N582|A0A482N582_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_HdSG1 OX=2508191 GN=HdSG1_00013 PE=4 SV=1
MM1 pKa = 6.89EE2 pKa = 5.56TIVLNCWVDD11 pKa = 3.71LDD13 pKa = 3.72EE14 pKa = 6.87HH15 pKa = 6.58EE16 pKa = 4.49YY17 pKa = 10.79CVNINNGPVPDD28 pKa = 4.08ATFASYY34 pKa = 11.5DD35 pKa = 3.51EE36 pKa = 3.86MDD38 pKa = 3.95AYY40 pKa = 11.45VKK42 pKa = 10.46GFRR45 pKa = 11.84EE46 pKa = 4.39CARR49 pKa = 11.84LANINVTVILPEE61 pKa = 3.82

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482N806|A0A482N806_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_HdSG1 OX=2508191 GN=HdSG1_00086 PE=4 SV=1
MM1 pKa = 7.32QKK3 pKa = 9.51HH4 pKa = 4.17QPLYY8 pKa = 9.88KK9 pKa = 9.88VRR11 pKa = 11.84RR12 pKa = 11.84LGGGLYY18 pKa = 10.3AVEE21 pKa = 4.05RR22 pKa = 11.84NKK24 pKa = 10.74RR25 pKa = 11.84SITIVSVKK33 pKa = 9.8VGSRR37 pKa = 11.84KK38 pKa = 9.39FYY40 pKa = 10.85SVPNCSPLLPTLRR53 pKa = 11.84DD54 pKa = 3.11AVLYY58 pKa = 10.52VLTGTFFF65 pKa = 4.14

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

18612

47

1086

216.4

24.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.387 ± 0.615

1.397 ± 0.148

5.668 ± 0.207

6.576 ± 0.31

3.476 ± 0.181

7.162 ± 0.302

1.907 ± 0.172

6.034 ± 0.161

6.055 ± 0.264

7.345 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.891 ± 0.171

4.959 ± 0.178

4.051 ± 0.238

4.696 ± 0.376

5.061 ± 0.217

6.163 ± 0.282

6.179 ± 0.278

7.028 ± 0.24

1.424 ± 0.106

3.541 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski