Cellulophaga algicola (strain DSM 14237 / IC166 / ACAM 630)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Cellulophaga; Cellulophaga algicola

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6X867|E6X867_CELAD Transcriptional regulator AraC family OS=Cellulophaga algicola (strain DSM 14237 / IC166 / ACAM 630) OX=688270 GN=Celal_2403 PE=4 SV=1
MM1 pKa = 7.39SVTPTTEE8 pKa = 4.58CLDD11 pKa = 3.67SDD13 pKa = 4.08NDD15 pKa = 4.02GVPDD19 pKa = 4.58VFDD22 pKa = 5.52LDD24 pKa = 3.82SDD26 pKa = 3.77NDD28 pKa = 4.58GIYY31 pKa = 10.79DD32 pKa = 3.65AVEE35 pKa = 4.13AGHH38 pKa = 6.05NQAHH42 pKa = 6.35TDD44 pKa = 3.73GVVTGAVGTDD54 pKa = 3.48GVPDD58 pKa = 3.68NVQNDD63 pKa = 3.99PNRR66 pKa = 11.84EE67 pKa = 4.03TVNYY71 pKa = 8.24TLSDD75 pKa = 3.75SDD77 pKa = 5.46LDD79 pKa = 4.2TIPDD83 pKa = 3.6VLEE86 pKa = 4.3FDD88 pKa = 5.13SDD90 pKa = 3.78NDD92 pKa = 3.76GCNDD96 pKa = 3.16SDD98 pKa = 3.67EE99 pKa = 5.36AYY101 pKa = 10.18GAKK104 pKa = 9.48DD105 pKa = 3.22TDD107 pKa = 3.43SDD109 pKa = 4.23ANGFYY114 pKa = 10.91GSGQPNVDD122 pKa = 3.13VNGRR126 pKa = 11.84ITAATYY132 pKa = 9.26PEE134 pKa = 4.93PNDD137 pKa = 3.58GDD139 pKa = 4.38SNTVYY144 pKa = 10.54DD145 pKa = 4.2YY146 pKa = 11.42KK147 pKa = 10.81EE148 pKa = 4.17KK149 pKa = 10.67KK150 pKa = 8.43QAPIIADD157 pKa = 3.63KK158 pKa = 11.55NNTTIQACYY167 pKa = 8.85STDD170 pKa = 3.03VTLINSALYY179 pKa = 10.66ADD181 pKa = 4.42TFQWQLLNGSNWIDD195 pKa = 3.08ISDD198 pKa = 3.69STKK201 pKa = 10.68YY202 pKa = 10.68SGTGTNTLDD211 pKa = 4.0IINVTLTEE219 pKa = 4.18NGNQYY224 pKa = 11.1RR225 pKa = 11.84LIASHH230 pKa = 6.88SSTICDD236 pKa = 3.47EE237 pKa = 4.68DD238 pKa = 4.43SSGVTTLNVNDD249 pKa = 4.32EE250 pKa = 3.86MDD252 pKa = 3.9APVSGGDD259 pKa = 3.28QSYY262 pKa = 11.17CSGDD266 pKa = 3.98SIPQLSANVPSDD278 pKa = 3.62EE279 pKa = 4.35TVDD282 pKa = 3.23WYY284 pKa = 11.25ANLSGGTALLEE295 pKa = 4.41SSLSYY300 pKa = 10.54TPAGAGTYY308 pKa = 8.72YY309 pKa = 11.1AEE311 pKa = 4.42ARR313 pKa = 11.84STTFVGCTSTTRR325 pKa = 11.84TPITLTEE332 pKa = 4.04EE333 pKa = 4.26SPSVVTIGADD343 pKa = 2.98QVVFVGDD350 pKa = 3.54NAIFTATASNSDD362 pKa = 3.52TFHH365 pKa = 6.86WEE367 pKa = 3.45VSTDD371 pKa = 3.16GGITFNSVAEE381 pKa = 4.14SSEE384 pKa = 4.02YY385 pKa = 10.65TGTQTVTLTVVSARR399 pKa = 11.84ALQNGYY405 pKa = 9.09RR406 pKa = 11.84FRR408 pKa = 11.84FVASTAGSSCGTTNSSSAVLTVKK431 pKa = 10.52VKK433 pKa = 9.52TVITNRR439 pKa = 11.84RR440 pKa = 11.84ITYY443 pKa = 9.56RR444 pKa = 11.84VKK446 pKa = 10.88KK447 pKa = 10.21NN448 pKa = 3.02

Molecular weight: 47.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6XCM7|E6XCM7_CELAD Glutamate 5-kinase OS=Cellulophaga algicola (strain DSM 14237 / IC166 / ACAM 630) OX=688270 GN=proB PE=3 SV=1
MM1 pKa = 7.5AKK3 pKa = 10.25EE4 pKa = 3.96SMKK7 pKa = 10.52ARR9 pKa = 11.84EE10 pKa = 4.29VKK12 pKa = 10.32RR13 pKa = 11.84EE14 pKa = 3.88KK15 pKa = 10.1TVAKK19 pKa = 10.55YY20 pKa = 10.61ADD22 pKa = 3.25KK23 pKa = 11.03RR24 pKa = 11.84KK25 pKa = 9.78ALKK28 pKa = 10.38AAGDD32 pKa = 3.87YY33 pKa = 10.79EE34 pKa = 4.59GLQKK38 pKa = 10.72LPRR41 pKa = 11.84NANPIRR47 pKa = 11.84LHH49 pKa = 5.75NRR51 pKa = 11.84CKK53 pKa = 10.01LTGRR57 pKa = 11.84PRR59 pKa = 11.84GYY61 pKa = 9.14MRR63 pKa = 11.84TFGVSRR69 pKa = 11.84VTFRR73 pKa = 11.84EE74 pKa = 3.87MANEE78 pKa = 3.69GLIPGVRR85 pKa = 11.84KK86 pKa = 10.15ASWW89 pKa = 2.78

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4114

0

4114

1411819

30

6081

343.2

38.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.583 ± 0.043

0.736 ± 0.011

5.729 ± 0.059

6.635 ± 0.046

5.184 ± 0.033

6.418 ± 0.05

1.676 ± 0.02

8.053 ± 0.036

7.769 ± 0.067

9.359 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.021

6.085 ± 0.042

3.296 ± 0.027

3.154 ± 0.023

3.14 ± 0.028

6.595 ± 0.035

6.224 ± 0.071

6.127 ± 0.037

1.045 ± 0.015

4.105 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski