Cellulosimicrobium sp. MM

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Cellulosimicrobium; unclassified Cellulosimicrobium

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2081 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085LF06|A0A085LF06_9MICO Sugar ABC transporter permease OS=Cellulosimicrobium sp. MM OX=1523621 GN=IU11_10360 PE=3 SV=1
MM1 pKa = 7.67LPRR4 pKa = 11.84STRR7 pKa = 11.84RR8 pKa = 11.84PLVLVVSAVALALTASGCGAILDD31 pKa = 4.91EE32 pKa = 4.78ISGPADD38 pKa = 3.38AQRR41 pKa = 11.84DD42 pKa = 3.97EE43 pKa = 4.86PGGEE47 pKa = 3.94VTEE50 pKa = 5.12ASDD53 pKa = 3.9ADD55 pKa = 3.97VFSVQVGDD63 pKa = 4.87CIVSSKK69 pKa = 11.03LDD71 pKa = 3.8DD72 pKa = 4.64ADD74 pKa = 3.97LVEE77 pKa = 4.73SVPVVPCSEE86 pKa = 3.89PHH88 pKa = 6.18DD89 pKa = 4.07AEE91 pKa = 4.84VYY93 pKa = 11.07AEE95 pKa = 4.23TEE97 pKa = 4.26LPAGNYY103 pKa = 9.71PGDD106 pKa = 3.7EE107 pKa = 4.23AVTTSAEE114 pKa = 4.16EE115 pKa = 3.76FCYY118 pKa = 10.36GAFEE122 pKa = 4.53PFVGLPYY129 pKa = 10.49EE130 pKa = 4.57EE131 pKa = 4.95SAHH134 pKa = 5.85EE135 pKa = 3.92YY136 pKa = 10.0WYY138 pKa = 7.59FTPRR142 pKa = 11.84ADD144 pKa = 2.66GWTRR148 pKa = 11.84QGDD151 pKa = 3.72RR152 pKa = 11.84VVQCVLDD159 pKa = 3.86TNGTDD164 pKa = 3.38VTGSLQGSATT174 pKa = 3.48

Molecular weight: 18.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085LH42|A0A085LH42_9MICO Uncharacterized protein OS=Cellulosimicrobium sp. MM OX=1523621 GN=IU11_03715 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.97GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2081

0

2081

538045

36

1656

258.6

27.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.274 ± 0.088

0.589 ± 0.015

6.561 ± 0.048

5.649 ± 0.055

2.51 ± 0.032

9.356 ± 0.055

1.974 ± 0.031

2.766 ± 0.037

1.65 ± 0.037

9.838 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.544 ± 0.022

1.499 ± 0.024

5.991 ± 0.049

2.488 ± 0.03

8.245 ± 0.069

5.105 ± 0.049

6.341 ± 0.048

10.37 ± 0.057

1.463 ± 0.025

1.786 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski