Azospirillum lipoferum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Azospirillum

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7NY64|A0A1X7NY64_AZOLI Plasmid segregation centromere-binding protein ParG OS=Azospirillum lipoferum OX=193 GN=SAMN02982994_1873 PE=4 SV=1
MM1 pKa = 7.04STDD4 pKa = 3.3ATEE7 pKa = 4.51LAPNAAPTPSADD19 pKa = 3.19LAYY22 pKa = 10.59YY23 pKa = 9.86IAALLPVGTPKK34 pKa = 10.16WGVATPGNAASVTFSFMEE52 pKa = 4.35SVPSYY57 pKa = 11.51ADD59 pKa = 3.37AGDD62 pKa = 3.77AAGFAPMNATQRR74 pKa = 11.84AAVRR78 pKa = 11.84QALAAWTEE86 pKa = 4.04VANITFTEE94 pKa = 4.42VADD97 pKa = 4.45GGAGGQLRR105 pKa = 11.84FGTNSQGGVSSGYY118 pKa = 10.59GYY120 pKa = 10.92YY121 pKa = 10.22PNASLAAGGDD131 pKa = 3.6VYY133 pKa = 10.99LAKK136 pKa = 10.4DD137 pKa = 3.43ASSNTSPTAGTYY149 pKa = 9.5GYY151 pKa = 7.94EE152 pKa = 4.37TIVHH156 pKa = 6.9EE157 pKa = 4.54IGHH160 pKa = 6.9AIGLKK165 pKa = 10.61HH166 pKa = 6.53PGNYY170 pKa = 9.14NAGGGGTEE178 pKa = 4.36APYY181 pKa = 10.94LPTAEE186 pKa = 5.88DD187 pKa = 3.51STQYY191 pKa = 11.12TVMSYY196 pKa = 11.19NDD198 pKa = 4.04HH199 pKa = 7.3PSLGLNGLLTGPVLYY214 pKa = 10.49DD215 pKa = 3.52IAAIQYY221 pKa = 10.32LYY223 pKa = 10.85GANTTTRR230 pKa = 11.84SGNTTYY236 pKa = 10.72SYY238 pKa = 11.53NSAATVVSGSIWDD251 pKa = 3.73AGGTDD256 pKa = 3.48TLDD259 pKa = 3.97ASGQTASVVINLAPGSFSSIGPNGSGGRR287 pKa = 11.84AVANLSIAYY296 pKa = 7.38GTTIEE301 pKa = 4.25NANGGSGDD309 pKa = 4.2DD310 pKa = 4.34SISGNAIDD318 pKa = 4.86NVLNGGGGNDD328 pKa = 4.0TISGGGGADD337 pKa = 3.43SIIVNGGIVTVDD349 pKa = 3.17GGSGIDD355 pKa = 3.51TLTIGAIGTVVRR367 pKa = 11.84ATGLEE372 pKa = 3.97NFIGGAGQDD381 pKa = 3.23RR382 pKa = 11.84VILIDD387 pKa = 3.48VGNTIVMSAIDD398 pKa = 3.81TLVGSGGNDD407 pKa = 2.85VVTLGTVANYY417 pKa = 10.57SIIAGIEE424 pKa = 4.02TLIGGAATDD433 pKa = 4.53IIVLANGGGTLIATGIEE450 pKa = 4.17YY451 pKa = 10.67LIGGAGQDD459 pKa = 2.99IAMLAGTGSTLITRR473 pKa = 11.84GIEE476 pKa = 4.04TLVGSTGQDD485 pKa = 2.46IVTLGDD491 pKa = 3.62GGNTMLAFAGLEE503 pKa = 3.93YY504 pKa = 10.59LIGGAGVDD512 pKa = 4.04AVTLGPGGNTIIVRR526 pKa = 11.84GLEE529 pKa = 4.14TLTGGAGNDD538 pKa = 3.03VVTFGNSGNLTTVEE552 pKa = 4.12DD553 pKa = 4.34LEE555 pKa = 4.8TIVGGTGTDD564 pKa = 3.39IILTGARR571 pKa = 11.84GSTLITAGIEE581 pKa = 4.01YY582 pKa = 10.25LIGGAGQDD590 pKa = 2.99IAMLAGTGNTLITRR604 pKa = 11.84GVEE607 pKa = 4.14TLVGSAGQDD616 pKa = 3.31VIMLGDD622 pKa = 4.16GGNTLTAFAAIEE634 pKa = 4.05YY635 pKa = 10.09LVGGAANDD643 pKa = 3.91LVLLGAGGSTVTVRR657 pKa = 11.84GLEE660 pKa = 4.07TLIGGAGIDD669 pKa = 3.88AVTLGNSYY677 pKa = 11.31NFITVGATEE686 pKa = 4.31TLTGGTSTDD695 pKa = 3.02IVVLAAGGSTLIATGLEE712 pKa = 4.12YY713 pKa = 10.8LIGGAGIDD721 pKa = 3.56IVALANGGNTVIVRR735 pKa = 11.84GLEE738 pKa = 3.99TLAGGIGTDD747 pKa = 3.95SLILGNSANFMTIGGIEE764 pKa = 4.36TLTGGTSTDD773 pKa = 2.69IVVLNDD779 pKa = 3.37AGSTMVASGIEE790 pKa = 3.98YY791 pKa = 10.38LIGGGAMDD799 pKa = 4.42VVTLGAAGTLVTRR812 pKa = 11.84GVEE815 pKa = 4.45TIAGSAGMDD824 pKa = 3.44TLILGDD830 pKa = 3.83STNTLTVTGVEE841 pKa = 4.21TLVGGVSADD850 pKa = 3.65TVTVAAGAIRR860 pKa = 11.84FEE862 pKa = 4.61GGGGADD868 pKa = 3.69AVTLAAGQAGDD879 pKa = 3.15RR880 pKa = 11.84VVFRR884 pKa = 11.84SVGDD888 pKa = 3.4GGGPGASVGYY898 pKa = 10.52DD899 pKa = 3.25RR900 pKa = 11.84ITNFGTGDD908 pKa = 3.49AVVVAGQMAGFVDD921 pKa = 5.0RR922 pKa = 11.84NANGALQTAVRR933 pKa = 11.84NTNQINLVTDD943 pKa = 3.7EE944 pKa = 4.71AVLLSVALASLTDD957 pKa = 3.42EE958 pKa = 4.66GFVNLRR964 pKa = 11.84AGIGKK969 pKa = 8.67MAGGVAGISALAVASDD985 pKa = 3.97GVDD988 pKa = 2.81TGVYY992 pKa = 10.37LITDD996 pKa = 3.81GDD998 pKa = 4.07GDD1000 pKa = 3.58GWLAAGEE1007 pKa = 4.12VRR1009 pKa = 11.84LLGLFGAAGIGASQLLAGG1027 pKa = 4.64

Molecular weight: 100.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7Q0R6|A0A1X7Q0R6_AZOLI 2-hydroxychromene-2-carboxylate isomerase OS=Azospirillum lipoferum OX=193 GN=SAMN02982994_4328 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIVRR12 pKa = 11.84KK13 pKa = 9.1HH14 pKa = 4.0RR15 pKa = 11.84HH16 pKa = 4.06GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.3VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91VLSAA44 pKa = 4.11

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6618

0

6618

2258444

39

6408

341.3

36.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.647 ± 0.05

0.805 ± 0.01

5.787 ± 0.031

5.377 ± 0.03

3.29 ± 0.019

9.247 ± 0.048

2.029 ± 0.015

4.258 ± 0.026

2.567 ± 0.024

10.79 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.017

2.281 ± 0.017

5.686 ± 0.036

2.823 ± 0.019

7.881 ± 0.042

4.959 ± 0.024

5.45 ± 0.043

7.673 ± 0.025

1.287 ± 0.013

1.919 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski