Cyanophage S-SSM6a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4PNE8|M4PNE8_9CAUD Uncharacterized protein OS=Cyanophage S-SSM6a OX=682650 GN=CYVG_00194 PE=4 SV=1
MM1 pKa = 7.2FKK3 pKa = 10.79SVIAATAAAPLFAGAAIAGPYY24 pKa = 9.93VNVEE28 pKa = 4.34TNSSFNGGDD37 pKa = 3.51YY38 pKa = 9.62TATTAEE44 pKa = 4.08FALGYY49 pKa = 10.14EE50 pKa = 4.26GSNWFVQGGPVVTAPDD66 pKa = 3.85AGTNDD71 pKa = 3.3TDD73 pKa = 4.2FLAKK77 pKa = 10.51AGGSVALSEE86 pKa = 4.61SVVGYY91 pKa = 10.99GEE93 pKa = 6.0LSFQTDD99 pKa = 4.15DD100 pKa = 3.01VDD102 pKa = 3.26NDD104 pKa = 3.8YY105 pKa = 11.38GVKK108 pKa = 10.54AGVKK112 pKa = 9.71YY113 pKa = 10.68VFF115 pKa = 4.25

Molecular weight: 11.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4PZB0|M4PZB0_9CAUD Uncharacterized protein OS=Cyanophage S-SSM6a OX=682650 GN=CYVG_00276 PE=4 SV=1
MM1 pKa = 7.58AANNIKK7 pKa = 9.7QQTTLTTANGRR18 pKa = 11.84RR19 pKa = 11.84IKK21 pKa = 10.03YY22 pKa = 7.77TVLKK26 pKa = 10.1RR27 pKa = 11.84SNAGRR32 pKa = 11.84TMARR36 pKa = 11.84RR37 pKa = 11.84AWNNAAPKK45 pKa = 10.28GSFMHH50 pKa = 6.33QGMAVHH56 pKa = 6.91AASINTGSKK65 pKa = 9.36VSKK68 pKa = 10.7AII70 pKa = 3.65

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

304

0

304

74317

26

3007

244.5

27.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.112 ± 0.16

1.132 ± 0.096

6.779 ± 0.139

6.3 ± 0.269

4.44 ± 0.119

7.811 ± 0.302

1.791 ± 0.105

6.409 ± 0.142

6.549 ± 0.338

7.05 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.133

5.692 ± 0.153

4.077 ± 0.106

3.535 ± 0.083

3.921 ± 0.096

6.806 ± 0.189

7.031 ± 0.354

6.741 ± 0.156

1.264 ± 0.084

4.333 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski