Streptomyces actuosus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7068 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9NWC9|A0A2U9NWC9_STRAS Uncharacterized protein OS=Streptomyces actuosus OX=1885 GN=DMT42_04210 PE=4 SV=1
MM1 pKa = 7.71ADD3 pKa = 3.23MNAFEE8 pKa = 4.32CLGRR12 pKa = 11.84RR13 pKa = 11.84THH15 pKa = 6.35ARR17 pKa = 11.84NAALVAVGGALAASVWCWPLTAAYY41 pKa = 10.27ADD43 pKa = 3.5ATSAYY48 pKa = 10.22GEE50 pKa = 4.07WTMNGSTGQVSIPLAGFPAASVTTDD75 pKa = 2.68SSTSSVQSGTSAFLGPGTPVGARR98 pKa = 11.84YY99 pKa = 7.57GTSQGRR105 pKa = 11.84TYY107 pKa = 11.56VNLRR111 pKa = 11.84TAAGRR116 pKa = 11.84TPSTTTLTFEE126 pKa = 4.5SPTNAGTFAFTLGDD140 pKa = 3.25IDD142 pKa = 4.43ADD144 pKa = 4.02SVRR147 pKa = 11.84VSATGADD154 pKa = 4.08GQPLTAAEE162 pKa = 4.96LGWQGAFNYY171 pKa = 8.6CQSSPKK177 pKa = 9.75PSSCGPTGTDD187 pKa = 2.55TDD189 pKa = 3.87QPVWDD194 pKa = 4.83PATSTLIGNGADD206 pKa = 3.43TTGAAGWFQPTVPVTSITLTFSMQSGFPIYY236 pKa = 9.57QLWTSSLSSQIAGRR250 pKa = 11.84LASDD254 pKa = 4.35CGTPSGQEE262 pKa = 3.55VRR264 pKa = 11.84LLDD267 pKa = 3.85EE268 pKa = 4.68NGAPVLNPDD277 pKa = 3.91GTPVTTTTDD286 pKa = 3.01AQGNYY291 pKa = 9.52AFADD295 pKa = 3.62IAPGQYY301 pKa = 9.34QVSTTSADD309 pKa = 3.39YY310 pKa = 10.14TPQTNSLPADD320 pKa = 3.63TSDD323 pKa = 3.7GNSVTNADD331 pKa = 4.1LTLTCQTSPSPTPTDD346 pKa = 3.37TDD348 pKa = 3.82SPTPTSTDD356 pKa = 3.05TDD358 pKa = 4.16SPTPTPTDD366 pKa = 3.37TASPSPTDD374 pKa = 3.52TASPTPTEE382 pKa = 4.35TGSPTPTDD390 pKa = 3.56TASPTPTDD398 pKa = 3.64TASPTPTDD406 pKa = 4.28PGSPSPTDD414 pKa = 3.32TASPSPTDD422 pKa = 3.44SASPSPTGTSSPTPTDD438 pKa = 3.35TASPTPTGTGSPTPTGTGSPSPTGPGSPSPTGTGNPSPTGPGAPTPTGTGSATPTPTPTGTCSAEE503 pKa = 3.82APPAYY508 pKa = 9.85GDD510 pKa = 3.34PWEE513 pKa = 4.48PTACPGVTTTQPRR526 pKa = 11.84PPVSDD531 pKa = 3.7DD532 pKa = 3.5TPADD536 pKa = 3.71GQPPQLADD544 pKa = 3.35TGVDD548 pKa = 5.0GYY550 pKa = 11.29AAWAAGLAAVSMAATGGFVVLRR572 pKa = 11.84RR573 pKa = 11.84KK574 pKa = 7.47TAGGEE579 pKa = 3.99HH580 pKa = 6.67RR581 pKa = 4.72

Molecular weight: 57.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9P571|A0A2U9P571_STRAS 50S ribosomal protein L4 OS=Streptomyces actuosus OX=1885 GN=rplD PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7068

0

7068

2320790

26

6931

328.4

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.936 ± 0.045

0.783 ± 0.008

6.011 ± 0.025

5.645 ± 0.032

2.689 ± 0.017

9.497 ± 0.028

2.306 ± 0.015

2.925 ± 0.016

2.091 ± 0.026

10.432 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.642 ± 0.012

1.676 ± 0.018

6.218 ± 0.031

2.746 ± 0.018

8.248 ± 0.036

4.755 ± 0.024

6.177 ± 0.028

8.522 ± 0.03

1.554 ± 0.013

2.148 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski