Culex nigripalpus nucleopolyhedrovirus (isolate Florida/1997) (CuniNPV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Deltabaculovirus; unclassified Deltabaculovirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q919K2|Q919K2_NPVCO CUN074 putative p74 envelope protein similar to AcMNPV ORF138 OS=Culex nigripalpus nucleopolyhedrovirus (isolate Florida/1997) OX=645993 GN=CUN074 PE=4 SV=1
MM1 pKa = 7.3IVTVFLHH8 pKa = 7.25DD9 pKa = 3.67EE10 pKa = 4.16SKK12 pKa = 11.13YY13 pKa = 8.18EE14 pKa = 3.97HH15 pKa = 6.31EE16 pKa = 4.6TFDD19 pKa = 3.82EE20 pKa = 4.35VRR22 pKa = 11.84VDD24 pKa = 4.02LPANVVKK31 pKa = 10.55IVVEE35 pKa = 4.19SHH37 pKa = 6.1TDD39 pKa = 3.23AQITVVDD46 pKa = 3.87SSGKK50 pKa = 9.39IVEE53 pKa = 4.23DD54 pKa = 4.16VYY56 pKa = 11.65VEE58 pKa = 4.09EE59 pKa = 5.96SSAQSYY65 pKa = 9.78KK66 pKa = 10.08PWRR69 pKa = 11.84DD70 pKa = 2.84LSEE73 pKa = 4.6FEE75 pKa = 4.08RR76 pKa = 11.84QLAYY80 pKa = 9.85FVYY83 pKa = 10.15KK84 pKa = 10.55GVVCIDD90 pKa = 3.45YY91 pKa = 9.63EE92 pKa = 4.83GPGILHH98 pKa = 7.3CIPEE102 pKa = 4.5PGYY105 pKa = 10.52KK106 pKa = 9.9RR107 pKa = 11.84PDD109 pKa = 3.17VEE111 pKa = 4.31TRR113 pKa = 11.84ALKK116 pKa = 10.76YY117 pKa = 9.82HH118 pKa = 7.15DD119 pKa = 4.74EE120 pKa = 4.37YY121 pKa = 11.55KK122 pKa = 10.61SAAGACTPEE131 pKa = 4.59LDD133 pKa = 3.89EE134 pKa = 4.8MDD136 pKa = 3.99IEE138 pKa = 4.84EE139 pKa = 4.48IDD141 pKa = 3.7YY142 pKa = 10.43MNLEE146 pKa = 4.26FVEE149 pKa = 4.5AWFEE153 pKa = 4.09GVEE156 pKa = 4.34EE157 pKa = 4.14IPEE160 pKa = 3.97FVPEE164 pKa = 4.02YY165 pKa = 10.5DD166 pKa = 3.45SSLFTTKK173 pKa = 10.41YY174 pKa = 11.1ANLQALIEE182 pKa = 4.35DD183 pKa = 4.57LL184 pKa = 4.34

Molecular weight: 21.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q919P7|Q919P7_NPVCO Uncharacterized protein CUN022 OS=Culex nigripalpus nucleopolyhedrovirus (isolate Florida/1997) OX=645993 GN=CUN022 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84TVVVLPNRR11 pKa = 11.84QAVSSGGRR19 pKa = 11.84KK20 pKa = 9.13RR21 pKa = 11.84SRR23 pKa = 11.84SRR25 pKa = 11.84SGCRR29 pKa = 11.84TRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84STSRR37 pKa = 11.84TRR39 pKa = 11.84TTKK42 pKa = 10.34RR43 pKa = 11.84KK44 pKa = 9.97APAKK48 pKa = 9.08RR49 pKa = 11.84ARR51 pKa = 11.84SRR53 pKa = 11.84SRR55 pKa = 11.84SRR57 pKa = 11.84SRR59 pKa = 11.84GRR61 pKa = 11.84GRR63 pKa = 11.84KK64 pKa = 8.54YY65 pKa = 10.91

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

32795

65

1769

300.9

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.083 ± 0.153

2.049 ± 0.14

5.376 ± 0.122

6.986 ± 0.284

3.909 ± 0.158

5.354 ± 0.157

2.305 ± 0.131

5.153 ± 0.165

4.815 ± 0.27

9.462 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.109

5.175 ± 0.16

4.992 ± 0.251

3.635 ± 0.178

7.175 ± 0.189

6.699 ± 0.159

5.617 ± 0.161

7.818 ± 0.181

0.97 ± 0.078

3.638 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski