Lutzomyia reovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; unclassified Reoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4M970|A0A0H4M970_9REOV RNA-dependent RNA polymerase OS=Lutzomyia reovirus 1 OX=1670669 PE=4 SV=1
MM1 pKa = 7.41MNFDD5 pKa = 4.18STIHH9 pKa = 6.51TIPIFFNLLTVLTTATIDD27 pKa = 3.4RR28 pKa = 11.84DD29 pKa = 3.86GLYY32 pKa = 10.32KK33 pKa = 10.54SYY35 pKa = 10.91ASPVSPSTIPAEE47 pKa = 4.57FIVPLAPPMLQLLNYY62 pKa = 7.26KK63 pKa = 9.25TSPQRR68 pKa = 11.84PLMVLQSFTAALWNSSNYY86 pKa = 9.28HH87 pKa = 5.75RR88 pKa = 11.84LKK90 pKa = 10.3IPRR93 pKa = 11.84SIVDD97 pKa = 3.2ITRR100 pKa = 11.84LASDD104 pKa = 3.5MTQWPWLQLLLDD116 pKa = 4.51CTNNWNYY123 pKa = 9.78TPEE126 pKa = 4.11FKK128 pKa = 10.85ALLVSVCHH136 pKa = 6.66AIEE139 pKa = 3.92IVVIGSVASNIANTPSTYY157 pKa = 10.31EE158 pKa = 4.02FEE160 pKa = 4.61SPSYY164 pKa = 9.01TLPSTYY170 pKa = 10.75DD171 pKa = 3.49PDD173 pKa = 3.93KK174 pKa = 11.04LTITLPRR181 pKa = 11.84MSMICKK187 pKa = 10.04LLQNILIPALEE198 pKa = 3.92RR199 pKa = 11.84VYY201 pKa = 11.18NIIDD205 pKa = 3.64VVDD208 pKa = 4.13DD209 pKa = 3.73VTPQFPTPPTSPILQEE225 pKa = 4.11SLSDD229 pKa = 3.41EE230 pKa = 4.98DD231 pKa = 4.03EE232 pKa = 4.28ATNTDD237 pKa = 2.82VRR239 pKa = 11.84YY240 pKa = 8.81QAYY243 pKa = 8.64YY244 pKa = 9.62AQRR247 pKa = 11.84ALSRR251 pKa = 11.84ICDD254 pKa = 3.89QITDD258 pKa = 5.46AIPCGSHH265 pKa = 7.13LLLSDD270 pKa = 4.4AAPDD274 pKa = 3.49VVIFYY279 pKa = 7.42FTNGNDD285 pKa = 3.32VFIGKK290 pKa = 7.68VEE292 pKa = 4.14RR293 pKa = 11.84SKK295 pKa = 9.9QTTLEE300 pKa = 3.87FEE302 pKa = 5.0YY303 pKa = 10.66IRR305 pKa = 11.84IAHH308 pKa = 6.97IDD310 pKa = 3.6QEE312 pKa = 4.44QAALKK317 pKa = 10.3AIQHH321 pKa = 5.46NVNLFRR327 pKa = 11.84NQLTQPEE334 pKa = 4.74LRR336 pKa = 11.84TLFSDD341 pKa = 4.11EE342 pKa = 4.37SVSMILSHH350 pKa = 5.51THH352 pKa = 6.69FIYY355 pKa = 10.38IPIHH359 pKa = 5.25EE360 pKa = 4.71RR361 pKa = 11.84PLRR364 pKa = 11.84EE365 pKa = 4.22IINGVIASVALIPSQALVTSKK386 pKa = 11.13GSS388 pKa = 3.15

Molecular weight: 43.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4LY56|A0A0H4LY56_9REOV VP1 OS=Lutzomyia reovirus 1 OX=1670669 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.98LLDD5 pKa = 4.55TEE7 pKa = 5.75DD8 pKa = 3.54STTQHH13 pKa = 5.6IRR15 pKa = 11.84KK16 pKa = 9.0QKK18 pKa = 10.71GLFKK22 pKa = 10.35KK23 pKa = 10.58SKK25 pKa = 9.13ISEE28 pKa = 4.57TITIQQRR35 pKa = 11.84YY36 pKa = 8.4SNIHH40 pKa = 4.88TQHH43 pKa = 5.92KK44 pKa = 8.28TVNLPTNHH52 pKa = 6.42VITLYY57 pKa = 8.68PHH59 pKa = 7.4RR60 pKa = 11.84ITTHH64 pKa = 3.96IHH66 pKa = 4.09QVLRR70 pKa = 11.84KK71 pKa = 9.4IYY73 pKa = 9.97GRR75 pKa = 11.84LDD77 pKa = 3.23AYY79 pKa = 10.02IDD81 pKa = 3.42PRR83 pKa = 11.84QIARR87 pKa = 11.84TLQSGEE93 pKa = 4.22RR94 pKa = 11.84YY95 pKa = 8.81RR96 pKa = 11.84QAVHH100 pKa = 6.44KK101 pKa = 10.05LKK103 pKa = 10.71SKK105 pKa = 10.53RR106 pKa = 11.84IRR108 pKa = 11.84NTSSAKK114 pKa = 6.99QTYY117 pKa = 9.49IFSVDD122 pKa = 3.66DD123 pKa = 4.16KK124 pKa = 11.08LSNDD128 pKa = 3.28ATQEE132 pKa = 3.99EE133 pKa = 5.1LDD135 pKa = 3.8QFQKK139 pKa = 11.05DD140 pKa = 3.97VNHH143 pKa = 6.74LADD146 pKa = 3.8IRR148 pKa = 11.84TQITTGLIFIHH159 pKa = 6.61LGYY162 pKa = 10.35HH163 pKa = 6.79EE164 pKa = 4.7YY165 pKa = 10.62HH166 pKa = 6.9CAIQPYY172 pKa = 8.7MRR174 pKa = 11.84ALMPYY179 pKa = 9.89LPIYY183 pKa = 9.31TCSLVNGFPNYY194 pKa = 10.26ADD196 pKa = 3.91PYY198 pKa = 9.56IANFYY203 pKa = 9.11LAPYY207 pKa = 8.34YY208 pKa = 10.65ASHH211 pKa = 6.54LTDD214 pKa = 4.6AYY216 pKa = 9.49TPKK219 pKa = 9.17PTTRR223 pKa = 11.84FIYY226 pKa = 10.1IDD228 pKa = 3.57TNLTARR234 pKa = 11.84QSIQVKK240 pKa = 9.25RR241 pKa = 11.84HH242 pKa = 5.18IKK244 pKa = 8.34YY245 pKa = 9.85QPSGTLITLSRR256 pKa = 11.84DD257 pKa = 2.97NSLPILFVSTKK268 pKa = 9.56HH269 pKa = 5.8LHH271 pKa = 6.26DD272 pKa = 4.3AKK274 pKa = 10.81EE275 pKa = 4.05YY276 pKa = 10.23IRR278 pKa = 11.84GKK280 pKa = 10.47RR281 pKa = 11.84LIEE284 pKa = 3.75LLYY287 pKa = 10.32DD288 pKa = 3.57YY289 pKa = 10.78SYY291 pKa = 10.0TAPHH295 pKa = 6.56QDD297 pKa = 2.58TTQRR301 pKa = 11.84IDD303 pKa = 3.71IISSAPLLTTITAYY317 pKa = 10.74SPTKK321 pKa = 9.35LQPYY325 pKa = 8.38PPPQCRR331 pKa = 11.84DD332 pKa = 3.02SALTVITKK340 pKa = 9.83FSHH343 pKa = 7.14HH344 pKa = 6.45IQNNHH349 pKa = 5.28QLTAILGVIGTKK361 pKa = 10.68GSGKK365 pKa = 8.07TAGLQRR371 pKa = 11.84AITFMQTTIPWTICHH386 pKa = 6.84IDD388 pKa = 3.15SDD390 pKa = 4.88AYY392 pKa = 10.54GRR394 pKa = 11.84WLAYY398 pKa = 10.35AITSTGTITEE408 pKa = 4.19ITQSFIDD415 pKa = 3.38DD416 pKa = 4.74HH417 pKa = 8.37IYY419 pKa = 11.12NEE421 pKa = 4.85YY422 pKa = 9.83KK423 pKa = 10.23QQDD426 pKa = 3.55NNSPIFHH433 pKa = 6.83EE434 pKa = 5.02LVMQKK439 pKa = 9.74ILNDD443 pKa = 3.92AKK445 pKa = 10.08ITTYY449 pKa = 11.06DD450 pKa = 3.1QFVNLSITQRR460 pKa = 11.84SNKK463 pKa = 9.46IYY465 pKa = 10.45EE466 pKa = 4.52PFTKK470 pKa = 10.23FYY472 pKa = 9.6ITTLSDD478 pKa = 3.3EE479 pKa = 4.83VYY481 pKa = 11.15GLANYY486 pKa = 7.29MTTLLAKK493 pKa = 9.08MDD495 pKa = 3.37HH496 pKa = 6.08RR497 pKa = 11.84VYY499 pKa = 10.38IIEE502 pKa = 4.21CHH504 pKa = 5.96TSVEE508 pKa = 3.91LAKK511 pKa = 10.43AVRR514 pKa = 11.84SDD516 pKa = 3.47TSCILDD522 pKa = 3.55TPFDD526 pKa = 3.65EE527 pKa = 5.17RR528 pKa = 11.84VAITHH533 pKa = 5.88RR534 pKa = 11.84TRR536 pKa = 11.84GDD538 pKa = 3.16KK539 pKa = 10.01TNKK542 pKa = 9.69LSQLVLHH549 pKa = 6.95EE550 pKa = 4.23YY551 pKa = 9.91YY552 pKa = 10.33RR553 pKa = 11.84ARR555 pKa = 11.84IAATIPYY562 pKa = 8.6VRR564 pKa = 11.84LYY566 pKa = 9.91EE567 pKa = 5.98LYY569 pKa = 9.42MCWTEE574 pKa = 4.54LSTSLLHH581 pKa = 6.96KK582 pKa = 10.04GVPQEE587 pKa = 4.15VMTPAPSNN595 pKa = 3.46

Molecular weight: 68.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

6911

246

1235

767.9

88.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.73 ± 0.352

1.129 ± 0.191

5.643 ± 0.191

4.269 ± 0.201

3.921 ± 0.341

3.82 ± 0.296

3.241 ± 0.389

9.072 ± 0.169

3.617 ± 0.28

9.333 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.176

5.687 ± 0.394

5.296 ± 0.264

5.31 ± 0.15

5.498 ± 0.25

7.061 ± 0.262

8.494 ± 0.435

4.341 ± 0.183

0.868 ± 0.135

5.426 ± 0.381
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski