Gordonia phage SketchMex

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Emalynvirus; unclassified Emalynvirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KQ34|A0A345KQ34_9CAUD Holliday junction resolvase OS=Gordonia phage SketchMex OX=2250418 GN=36 PE=4 SV=1
MM1 pKa = 7.62NEE3 pKa = 3.93LTNAIATTHH12 pKa = 6.69HH13 pKa = 6.99DD14 pKa = 4.93FIAALALDD22 pKa = 4.97DD23 pKa = 5.43GDD25 pKa = 4.18VALNCAFSSIEE36 pKa = 3.96DD37 pKa = 4.08LFAEE41 pKa = 4.57TADD44 pKa = 3.67VTPEE48 pKa = 3.88LSIDD52 pKa = 3.53YY53 pKa = 10.86ALLFTTAIVAADD65 pKa = 3.51SVYY68 pKa = 11.4ANYY71 pKa = 10.91

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KQ35|A0A345KQ35_9CAUD Uncharacterized protein OS=Gordonia phage SketchMex OX=2250418 GN=37 PE=4 SV=1
MM1 pKa = 7.06SQPEE5 pKa = 4.08ARR7 pKa = 11.84LGRR10 pKa = 11.84QIRR13 pKa = 11.84KK14 pKa = 9.32ALEE17 pKa = 3.49EE18 pKa = 3.92RR19 pKa = 11.84GAFMFKK25 pKa = 9.65IHH27 pKa = 7.03GGPTMMAGLPDD38 pKa = 5.8LIGVWHH44 pKa = 6.63GRR46 pKa = 11.84FIAIEE51 pKa = 3.93VKK53 pKa = 10.18MPGNSPSKK61 pKa = 8.68IQEE64 pKa = 3.69RR65 pKa = 11.84VMDD68 pKa = 5.46RR69 pKa = 11.84IRR71 pKa = 11.84QAGGRR76 pKa = 11.84VVVAYY81 pKa = 10.16SVADD85 pKa = 3.59ALEE88 pKa = 4.08VLRR91 pKa = 11.84ARR93 pKa = 11.84RR94 pKa = 11.84DD95 pKa = 3.21

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14363

31

1599

214.4

23.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.747 ± 0.475

0.794 ± 0.126

6.44 ± 0.334

6.259 ± 0.391

3.251 ± 0.188

8.014 ± 0.484

1.97 ± 0.18

4.874 ± 0.249

5.145 ± 0.258

8.139 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.785 ± 0.146

3.321 ± 0.176

5.222 ± 0.194

3.579 ± 0.166

6.461 ± 0.302

6.162 ± 0.259

5.876 ± 0.273

6.795 ± 0.234

2.235 ± 0.129

2.931 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski