Acetobacter nitrogenifigens DSM 23921 = LMG 23498

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acetobacter; Acetobacter nitrogenifigens

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A511XDQ0|A0A511XDQ0_9PROT Cysteine synthase OS=Acetobacter nitrogenifigens DSM 23921 = LMG 23498 OX=1120919 GN=cysK1 PE=4 SV=1
MM1 pKa = 7.7SIFNSLTTAVAGINAQSTAFTNLSNNIANSQTTGYY36 pKa = 9.63KK37 pKa = 10.43ASTTSFQDD45 pKa = 3.73FVSSSLRR52 pKa = 11.84SSSSDD57 pKa = 3.06AVSDD61 pKa = 3.78GVGAVTHH68 pKa = 7.0TSTQQQGTVSASTNSLAVAISGNGFFDD95 pKa = 3.44VSKK98 pKa = 10.74EE99 pKa = 4.07SGQATSGTEE108 pKa = 3.69RR109 pKa = 11.84FSDD112 pKa = 3.35QQYY115 pKa = 7.59YY116 pKa = 8.48TRR118 pKa = 11.84NGDD121 pKa = 3.34FYY123 pKa = 11.12EE124 pKa = 4.65DD125 pKa = 3.41KK126 pKa = 11.01NGYY129 pKa = 8.78LVNTSGYY136 pKa = 10.45YY137 pKa = 10.1LDD139 pKa = 5.91GYY141 pKa = 9.95QADD144 pKa = 4.09ATTGTLGTSLTQINVSNVVFRR165 pKa = 11.84PTEE168 pKa = 4.13TTKK171 pKa = 10.36LTMTGDD177 pKa = 3.58VGSTTLPSSGAATTTTSSTIYY198 pKa = 10.18DD199 pKa = 3.84SQSASHH205 pKa = 6.27TLDD208 pKa = 3.38VTWTQDD214 pKa = 2.95SANPLKK220 pKa = 9.33WTVSASDD227 pKa = 4.34PSDD230 pKa = 3.28SSAISATPYY239 pKa = 9.99TVTFDD244 pKa = 3.31SSGAIASVTDD254 pKa = 3.43SSGDD258 pKa = 3.52AVGSSLSGATASIPITANYY277 pKa = 10.0NGTSQSIALNIGTIGSSSATTLSTTSSTSTTPTLTSDD314 pKa = 3.32SVTSGTYY321 pKa = 9.8EE322 pKa = 4.07GVEE325 pKa = 4.14MEE327 pKa = 4.28SDD329 pKa = 3.49GSVMAEE335 pKa = 4.11FSNGDD340 pKa = 3.36TQLVGKK346 pKa = 9.98IALSSFANADD356 pKa = 3.32GLSAVDD362 pKa = 3.56GQAYY366 pKa = 10.44VGTAQSGSATTGVVGANGTGTLEE389 pKa = 4.17TSSVEE394 pKa = 4.4SSTTDD399 pKa = 3.23LTSDD403 pKa = 3.71LSGLIVAQEE412 pKa = 4.2AYY414 pKa = 8.18TANTKK419 pKa = 9.92VVTTANQLLQSTIAMIQQ436 pKa = 2.9

Molecular weight: 44.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A511X773|A0A511X773_9PROT Flavin prenyltransferase UbiX OS=Acetobacter nitrogenifigens DSM 23921 = LMG 23498 OX=1120919 GN=ubiX PE=3 SV=1
MM1 pKa = 7.26TLAPAQPSSVMPGRR15 pKa = 11.84DD16 pKa = 3.01WRR18 pKa = 11.84DD19 pKa = 3.01LRR21 pKa = 11.84PRR23 pKa = 11.84LLSALALIIVAVLAISLGGYY43 pKa = 10.34AYY45 pKa = 10.52GLLIIATMAGMAAEE59 pKa = 4.79GAALFALSPRR69 pKa = 11.84SWRR72 pKa = 11.84GALFVAWSFCAGLSAFCGRR91 pKa = 11.84WEE93 pKa = 4.05ATPAFAMTAFVFGPPLWLMNAVIAAGGLSLMWLRR127 pKa = 11.84LGADD131 pKa = 3.46CGVWAVVFVIAVVVSSDD148 pKa = 2.85SAAYY152 pKa = 8.25VTGRR156 pKa = 11.84MIGGPKK162 pKa = 9.63LAPRR166 pKa = 11.84ISPGKK171 pKa = 7.59TRR173 pKa = 11.84SGAVGGLLGAVMAGLVVATISGAGFAARR201 pKa = 11.84GAGWALLLGIAAQAGDD217 pKa = 3.87LAEE220 pKa = 4.42SAVKK224 pKa = 10.33RR225 pKa = 11.84ARR227 pKa = 11.84GVKK230 pKa = 10.19DD231 pKa = 3.08SGRR234 pKa = 11.84LLPGHH239 pKa = 6.75GGLLDD244 pKa = 4.13RR245 pKa = 11.84FDD247 pKa = 5.04ALLAAAPLAALLSLLAKK264 pKa = 10.08PGLPFWSAGFDD275 pKa = 3.52DD276 pKa = 4.95LARR279 pKa = 11.84GLGRR283 pKa = 11.84FLGGG287 pKa = 3.36

Molecular weight: 29.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3660

0

3660

1164225

41

2112

318.1

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.561 ± 0.051

0.919 ± 0.012

5.661 ± 0.03

5.22 ± 0.04

3.556 ± 0.026

8.665 ± 0.036

2.168 ± 0.019

4.777 ± 0.027

2.716 ± 0.031

10.048 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.017

2.632 ± 0.028

5.423 ± 0.032

3.075 ± 0.025

7.371 ± 0.044

6.119 ± 0.035

5.629 ± 0.031

7.452 ± 0.031

1.396 ± 0.016

2.226 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski