Lactococcus phage CHPC959

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus CHPC959

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650ET65|A0A650ET65_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC959 OX=2675255 GN=CHPC959_000911 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.11KK3 pKa = 8.64YY4 pKa = 9.83WVVEE8 pKa = 3.9DD9 pKa = 3.74HH10 pKa = 6.88LGGGFYY16 pKa = 10.68LMSEE20 pKa = 4.22DD21 pKa = 3.83TPEE24 pKa = 3.89EE25 pKa = 3.86EE26 pKa = 4.0LRR28 pKa = 11.84EE29 pKa = 4.04VEE31 pKa = 5.18VYY33 pKa = 10.89CDD35 pKa = 3.26TCGDD39 pKa = 3.59SDD41 pKa = 5.05SIIGQFSNWNQLKK54 pKa = 10.45KK55 pKa = 11.08EE56 pKa = 4.06MTDD59 pKa = 3.5DD60 pKa = 4.56EE61 pKa = 5.2GWCPYY66 pKa = 10.27SDD68 pKa = 4.78EE69 pKa = 4.14YY70 pKa = 11.18LKK72 pKa = 11.09SVFEE76 pKa = 4.32

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650ETA2|A0A650ETA2_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC959 OX=2675255 GN=CHPC959_000951 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.54SIANAQHH21 pKa = 5.85NKK23 pKa = 9.27KK24 pKa = 10.0KK25 pKa = 10.46AGKK28 pKa = 8.52IPVRR32 pKa = 11.84AYY34 pKa = 9.69HH35 pKa = 6.61CKK37 pKa = 8.93WCNLYY42 pKa = 10.43HH43 pKa = 7.32LSSQQRR49 pKa = 11.84LNIKK53 pKa = 9.21TGVIGG58 pKa = 3.97

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

8627

31

919

146.2

16.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.796 ± 0.534

0.788 ± 0.187

5.645 ± 0.37

7.616 ± 0.465

4.509 ± 0.294

6.283 ± 0.695

1.345 ± 0.209

6.711 ± 0.345

9.285 ± 0.457

8.821 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.185

6.596 ± 0.313

2.226 ± 0.333

3.477 ± 0.257

3.454 ± 0.264

6.248 ± 0.482

6.468 ± 0.3

6.283 ± 0.273

1.368 ± 0.14

4.382 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski