Buchnera aphidicola (Stegophylla sp.)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6Y992|A0A4D6Y992_9GAMM HPr family phosphocarrier protein OS=Buchnera aphidicola (Stegophylla sp.) OX=2315800 GN=D9V79_00245 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 6.14SINTDD7 pKa = 2.66ILYY10 pKa = 9.88FAASIMIGLAAIGASIGIGILGGKK34 pKa = 8.69FLEE37 pKa = 5.36GAARR41 pKa = 11.84QPDD44 pKa = 5.18LIPILRR50 pKa = 11.84TQFFIVMGLVDD61 pKa = 7.0AIPMIAVGLSLYY73 pKa = 10.33IIFSFSGG80 pKa = 3.05

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6Y8P8|A0A4D6Y8P8_9GAMM Dihydrolipoyl dehydrogenase OS=Buchnera aphidicola (Stegophylla sp.) OX=2315800 GN=lpdA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.51RR12 pKa = 11.84NRR14 pKa = 11.84VHH16 pKa = 7.35GFRR19 pKa = 11.84IRR21 pKa = 11.84MKK23 pKa = 9.28TKK25 pKa = 9.53NGRR28 pKa = 11.84HH29 pKa = 4.84ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.06KK37 pKa = 10.53LRR39 pKa = 11.84TRR41 pKa = 11.84LTVSSEE47 pKa = 3.72

Molecular weight: 5.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

354

0

354

112165

38

1394

316.9

36.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.525 ± 0.102

1.422 ± 0.047

3.938 ± 0.08

4.09 ± 0.116

4.863 ± 0.122

5.245 ± 0.096

2.614 ± 0.058

13.15 ± 0.151

9.912 ± 0.15

9.512 ± 0.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.055

7.733 ± 0.115

2.932 ± 0.055

3.97 ± 0.076

3.482 ± 0.093

6.61 ± 0.095

4.61 ± 0.09

4.557 ± 0.112

0.908 ± 0.054

4.527 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski