Cohnella sp. CC-MHH1044

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6462 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4BN37|A0A4S4BN37_9BACL LLM class flavin-dependent oxidoreductase OS=Cohnella sp. CC-MHH1044 OX=2565925 GN=E6C55_22090 PE=4 SV=1
MM1 pKa = 6.93VAHH4 pKa = 7.4LIGGYY9 pKa = 9.78SVTKK13 pKa = 10.18GKK15 pKa = 9.61WIGFGLLGLLALFVIWLFIPSEE37 pKa = 4.25DD38 pKa = 3.78TDD40 pKa = 3.52NTNVKK45 pKa = 10.27RR46 pKa = 11.84EE47 pKa = 3.88AAEE50 pKa = 3.75NDD52 pKa = 3.1GDD54 pKa = 4.22YY55 pKa = 11.1TILVYY60 pKa = 10.63MNGSDD65 pKa = 4.91LEE67 pKa = 4.23SEE69 pKa = 4.27YY70 pKa = 11.29DD71 pKa = 3.73EE72 pKa = 5.9EE73 pKa = 5.33SDD75 pKa = 3.87SYY77 pKa = 11.69YY78 pKa = 11.19GAASSDD84 pKa = 3.98LEE86 pKa = 4.14EE87 pKa = 4.82MISGLSGDD95 pKa = 3.69RR96 pKa = 11.84VRR98 pKa = 11.84VLVEE102 pKa = 3.84TGGTLAWADD111 pKa = 3.52EE112 pKa = 4.8RR113 pKa = 11.84IDD115 pKa = 4.6AEE117 pKa = 4.41QNQRR121 pKa = 11.84WLIEE125 pKa = 4.71DD126 pKa = 3.7GQFTHH131 pKa = 6.98AADD134 pKa = 3.3VGLRR138 pKa = 11.84NIGEE142 pKa = 4.64ADD144 pKa = 3.41TLADD148 pKa = 4.35FVAWGVEE155 pKa = 3.94QYY157 pKa = 10.64PADD160 pKa = 3.92KK161 pKa = 10.57YY162 pKa = 11.78ALIFWNHH169 pKa = 5.72GGGSVLGFGADD180 pKa = 3.04EE181 pKa = 4.49RR182 pKa = 11.84FDD184 pKa = 4.2YY185 pKa = 11.15DD186 pKa = 4.92SLTLDD191 pKa = 3.67EE192 pKa = 5.12LAAGLKK198 pKa = 7.08TAQMSTDD205 pKa = 2.94VSFEE209 pKa = 4.14VIGFDD214 pKa = 3.36ACLMANVEE222 pKa = 4.49TASLLSPYY230 pKa = 10.17GRR232 pKa = 11.84YY233 pKa = 9.18LVASEE238 pKa = 4.26EE239 pKa = 4.33LEE241 pKa = 4.55PGHH244 pKa = 6.16GWDD247 pKa = 3.45YY248 pKa = 10.36TAALSRR254 pKa = 11.84LSDD257 pKa = 3.6EE258 pKa = 4.74PDD260 pKa = 3.24ADD262 pKa = 3.96GATLGRR268 pKa = 11.84WIADD272 pKa = 4.06GYY274 pKa = 9.46RR275 pKa = 11.84QHH277 pKa = 8.41AEE279 pKa = 4.61DD280 pKa = 4.82NDD282 pKa = 3.63QEE284 pKa = 4.56KK285 pKa = 11.06NITLSVVDD293 pKa = 4.07MSRR296 pKa = 11.84MEE298 pKa = 4.22RR299 pKa = 11.84VVTALEE305 pKa = 4.22AFAQEE310 pKa = 4.09VDD312 pKa = 3.37SGISVDD318 pKa = 3.3RR319 pKa = 11.84SGFFSLANGRR329 pKa = 11.84SRR331 pKa = 11.84ADD333 pKa = 3.43EE334 pKa = 4.43YY335 pKa = 11.5GSSSTPEE342 pKa = 3.95EE343 pKa = 4.05ASDD346 pKa = 4.04MVDD349 pKa = 3.28LLAFVRR355 pKa = 11.84NVAEE359 pKa = 4.86RR360 pKa = 11.84YY361 pKa = 8.45PDD363 pKa = 3.37TSKK366 pKa = 10.96EE367 pKa = 4.04LEE369 pKa = 4.18NALQDD374 pKa = 3.41AVVYY378 pKa = 10.28NIVSVGHH385 pKa = 7.13PDD387 pKa = 3.22ASGLSIYY394 pKa = 10.24FPHH397 pKa = 6.99RR398 pKa = 11.84NKK400 pKa = 10.38INFDD404 pKa = 3.97DD405 pKa = 3.83NLQRR409 pKa = 11.84FAAIGFSDD417 pKa = 3.83KK418 pKa = 10.96YY419 pKa = 11.45VEE421 pKa = 3.95FLHH424 pKa = 6.65WYY426 pKa = 9.96VSGLLGNSEE435 pKa = 4.37TVEE438 pKa = 4.15VSTSGQLNFSYY449 pKa = 10.98NEE451 pKa = 4.11EE452 pKa = 3.89EE453 pKa = 5.15DD454 pKa = 4.01EE455 pKa = 4.24YY456 pKa = 11.59APYY459 pKa = 10.16EE460 pKa = 4.41VYY462 pKa = 10.83VDD464 pKa = 3.92PEE466 pKa = 3.99EE467 pKa = 5.1LEE469 pKa = 4.25QIEE472 pKa = 4.31QIYY475 pKa = 10.39AIVSMYY481 pKa = 10.64SDD483 pKa = 3.52EE484 pKa = 5.13DD485 pKa = 3.71AGEE488 pKa = 4.47DD489 pKa = 3.98SPLIYY494 pKa = 10.28LGYY497 pKa = 10.52DD498 pKa = 3.07HH499 pKa = 6.96YY500 pKa = 12.08VDD502 pKa = 4.12VDD504 pKa = 3.39WEE506 pKa = 4.13EE507 pKa = 4.46GIIRR511 pKa = 11.84DD512 pKa = 4.23DD513 pKa = 4.35FTGEE517 pKa = 3.16WLMWDD522 pKa = 3.83GNFVTLDD529 pKa = 3.84LVSQGDD535 pKa = 3.75GFIRR539 pKa = 11.84YY540 pKa = 8.4AIPAKK545 pKa = 10.84LNGKK549 pKa = 9.45DD550 pKa = 3.26VDD552 pKa = 3.84ILVHH556 pKa = 6.23YY557 pKa = 10.14DD558 pKa = 3.29VEE560 pKa = 4.51EE561 pKa = 4.05EE562 pKa = 4.17TFEE565 pKa = 5.42VLGAWKK571 pKa = 10.48GLGSEE576 pKa = 4.41TGMPDD581 pKa = 3.51KK582 pKa = 11.05NIIPIRR588 pKa = 11.84EE589 pKa = 3.9GDD591 pKa = 3.66RR592 pKa = 11.84IVPQFYY598 pKa = 10.12YY599 pKa = 10.66YY600 pKa = 10.64YY601 pKa = 10.8DD602 pKa = 3.84SGEE605 pKa = 4.21DD606 pKa = 3.57GYY608 pKa = 11.11MDD610 pKa = 3.67GDD612 pKa = 3.89EE613 pKa = 4.61FVVGDD618 pKa = 4.52TIDD621 pKa = 4.26LEE623 pKa = 4.26YY624 pKa = 10.75DD625 pKa = 3.46YY626 pKa = 10.97LPNGSYY632 pKa = 10.85LYY634 pKa = 10.74GFSLVSYY641 pKa = 10.07SGEE644 pKa = 3.85EE645 pKa = 3.89TLTDD649 pKa = 4.68FIEE652 pKa = 5.29FEE654 pKa = 4.73LEE656 pKa = 3.94DD657 pKa = 3.54

Molecular weight: 73.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4BIX2|A0A4S4BIX2_9BACL Carb-bd_dom_fam9 domain-containing protein OS=Cohnella sp. CC-MHH1044 OX=2565925 GN=E6C55_24620 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.17VHH16 pKa = 5.5GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.58NGRR28 pKa = 11.84KK29 pKa = 8.51VLKK32 pKa = 10.34ARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.21GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6462

0

6462

2442047

24

4735

377.9

41.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.687 ± 0.046

0.711 ± 0.009

5.4 ± 0.023

6.736 ± 0.037

3.879 ± 0.021

8.074 ± 0.037

1.795 ± 0.016

5.697 ± 0.026

4.269 ± 0.029

10.165 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.014

3.514 ± 0.027

4.275 ± 0.019

3.341 ± 0.017

5.991 ± 0.039

6.645 ± 0.03

5.454 ± 0.037

6.954 ± 0.026

1.432 ± 0.013

3.539 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski