Marmota monax (Woodchuck)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires;

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E4B012|A0A5E4B012_MARMO Prospero domain-containing protein OS=Marmota monax OX=9995 GN=MONAX_5E008480 PE=4 SV=1
MM1 pKa = 8.05EE2 pKa = 4.5DD3 pKa = 2.93TFYY6 pKa = 11.2HH7 pKa = 6.38SNCTDD12 pKa = 3.47KK13 pKa = 11.24DD14 pKa = 3.85DD15 pKa = 5.94CISNCKK21 pKa = 9.62PNLSLSGGYY30 pKa = 9.54CPYY33 pKa = 10.7KK34 pKa = 10.86DD35 pKa = 3.18RR36 pKa = 11.84TDD38 pKa = 3.32WKK40 pKa = 10.47DD41 pKa = 3.22SSSSEE46 pKa = 4.08DD47 pKa = 3.57STSEE51 pKa = 4.7AYY53 pKa = 9.69FCYY56 pKa = 10.22SLPPVQEE63 pKa = 4.23GQLTEE68 pKa = 4.3SEE70 pKa = 4.5SMTIGIRR77 pKa = 11.84EE78 pKa = 4.1QIHH81 pKa = 6.37EE82 pKa = 4.21SEE84 pKa = 4.42QLCGLPIVHH93 pKa = 6.22VVDD96 pKa = 4.92VYY98 pKa = 11.28LGSHH102 pKa = 6.11YY103 pKa = 10.9EE104 pKa = 4.08DD105 pKa = 4.4ALATLDD111 pKa = 4.48LHH113 pKa = 7.7RR114 pKa = 11.84DD115 pKa = 3.81YY116 pKa = 11.62QWMDD120 pKa = 3.31KK121 pKa = 10.37YY122 pKa = 11.09PKK124 pKa = 10.56GRR126 pKa = 11.84MRR128 pKa = 11.84KK129 pKa = 8.8ILWKK133 pKa = 9.83MEE135 pKa = 3.47VLMRR139 pKa = 11.84YY140 pKa = 9.79LKK142 pKa = 10.53VFPDD146 pKa = 4.07DD147 pKa = 5.83LKK149 pKa = 11.56DD150 pKa = 5.58DD151 pKa = 5.62DD152 pKa = 6.5DD153 pKa = 7.75DD154 pKa = 7.7DD155 pKa = 7.68DD156 pKa = 7.68DD157 pKa = 7.63DD158 pKa = 7.63DD159 pKa = 7.51DD160 pKa = 6.89DD161 pKa = 6.56DD162 pKa = 6.42NEE164 pKa = 6.21DD165 pKa = 3.57EE166 pKa = 5.93DD167 pKa = 6.02DD168 pKa = 4.22YY169 pKa = 12.05NDD171 pKa = 6.21DD172 pKa = 4.01EE173 pKa = 5.24DD174 pKa = 5.54HH175 pKa = 8.08DD176 pKa = 4.54NGDD179 pKa = 3.61YY180 pKa = 11.46DD181 pKa = 4.73EE182 pKa = 6.35DD183 pKa = 5.56KK184 pKa = 11.42DD185 pKa = 5.72DD186 pKa = 6.75DD187 pKa = 5.15DD188 pKa = 7.47DD189 pKa = 5.38EE190 pKa = 7.4DD191 pKa = 6.53DD192 pKa = 4.14YY193 pKa = 12.17DD194 pKa = 4.01EE195 pKa = 6.88DD196 pKa = 3.9EE197 pKa = 4.95DD198 pKa = 4.3NRR200 pKa = 11.84VYY202 pKa = 11.16DD203 pKa = 3.75EE204 pKa = 6.53DD205 pKa = 4.2EE206 pKa = 5.95DD207 pKa = 4.32EE208 pKa = 6.08DD209 pKa = 4.46EE210 pKa = 4.88DD211 pKa = 6.07DD212 pKa = 4.0EE213 pKa = 6.01VSMEE217 pKa = 4.66LLDD220 pKa = 4.52FSS222 pKa = 4.71

Molecular weight: 26.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E4CG38|A0A5E4CG38_MARMO DNA-directed RNA polymerase II subunit F OS=Marmota monax OX=9995 GN=MONAX_5E047116 PE=4 SV=1
AA1 pKa = 7.53RR2 pKa = 11.84RR3 pKa = 11.84SAGRR7 pKa = 11.84RR8 pKa = 11.84GSGSEE13 pKa = 3.87GAPRR17 pKa = 11.84SPALGASPPAPAPPRR32 pKa = 11.84PRR34 pKa = 11.84AAGPRR39 pKa = 11.84FRR41 pKa = 11.84QLFHH45 pKa = 6.3SRR47 pKa = 11.84SRR49 pKa = 11.84KK50 pKa = 9.08LSFRR54 pKa = 11.84FLAGARR60 pKa = 11.84GRR62 pKa = 11.84RR63 pKa = 11.84QQ64 pKa = 2.89

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30583

10698

41281

18551965

36

36136

449.4

49.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.019 ± 0.014

2.208 ± 0.011

4.731 ± 0.01

7.075 ± 0.017

3.533 ± 0.01

6.653 ± 0.018

2.608 ± 0.007

4.236 ± 0.012

5.604 ± 0.019

9.985 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.007

3.495 ± 0.009

6.56 ± 0.021

4.854 ± 0.016

5.725 ± 0.013

8.466 ± 0.018

5.335 ± 0.015

6.009 ± 0.014

1.211 ± 0.004

2.539 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski