Proteome-pI 2.0 - Proteome Isoelectric Point Database

Paenibacillaceae bacterium GAS479

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; unclassified Paenibacillaceae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 4781 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1H2B275|A0A1H2B275_9BACL Predicted dehydrogenase OS=Paenibacillaceae bacterium GAS479 OX=1882832 GN=SAMN05444162_4729 PE=4 SV=1

MM1 pKa = 7.6SYY3 pKa = 11.09KK4 pKa = 10.29NAEE7 pKa = 4.44IDD9 pKa = 4.43CPWCGEE15 pKa = 3.89AVVLDD20 pKa = 3.79GTICPDD26 pKa = 3.29CRR28 pKa = 11.84HH29 pKa = 5.81EE30 pKa = 4.49VLPEE34 pKa = 3.69HH35 pKa = 7.18LKK37 pKa = 9.56QQEE40 pKa = 4.34SGDD43 pKa = 3.9VEE45 pKa = 5.65DD46 pKa = 6.29DD47 pKa = 3.63VDD49 pKa = 5.79DD50 pKa = 4.37VDD52 pKa = 5.11ALAAEE57 pKa = 4.72SAEE60 pKa = 4.61PPDD63 pKa = 3.43PWTEE67 pKa = 4.01VEE69 pKa = 4.44NPEE72 pKa = 4.96AYY74 pKa = 10.52LLSKK78 pKa = 8.23FICRR82 pKa = 11.84GCGGSEE88 pKa = 3.56ASAEE92 pKa = 4.09EE93 pKa = 4.15VAMTGTGLSKK103 pKa = 10.79IMDD106 pKa = 4.24LQHH109 pKa = 5.26NHH111 pKa = 5.68YY112 pKa = 10.87LFVSCLNCGLVDD124 pKa = 4.13VYY126 pKa = 11.54DD127 pKa = 4.5PDD129 pKa = 3.66VLLRR133 pKa = 11.84RR134 pKa = 11.84ASGTTSNILDD144 pKa = 3.79LFFF147 pKa = 6.36

Molecular weight: 16.08 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1H1VF34|A0A1H1VF34_9BACL Uncharacterized protein OS=Paenibacillaceae bacterium GAS479 OX=1882832 GN=SAMN05444162_2388 PE=4 SV=1

MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSSKK25 pKa = 10.47NGRR28 pKa = 11.84KK29 pKa = 9.01VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.72GRR39 pKa = 11.84SKK41 pKa = 11.12LSAA44 pKa = 3.74

Molecular weight: 5.14 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
4781	0	4781	1546274	27	4762	323.4	35.73

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.955 ± 0.054	0.699 ± 0.013	4.96 ± 0.028	6.702 ± 0.041	3.917 ± 0.028	7.958 ± 0.037	1.822 ± 0.018	6.151 ± 0.034	4.89 ± 0.038	10.389 ± 0.05

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.71 ± 0.022	3.665 ± 0.031	4.355 ± 0.046	3.752 ± 0.024	5.506 ± 0.048	6.828 ± 0.041	5.355 ± 0.04	6.886 ± 0.047	1.264 ± 0.016	3.233 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski