Carrot red leaf luteovirus associated RNA

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 8.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O37365|O37365_9VIRU Uncharacterized protein OS=Carrot red leaf luteovirus associated RNA OX=67962 PE=4 SV=2
MM1 pKa = 7.49TNLNIEE7 pKa = 4.62SLVEE11 pKa = 3.87TQPAVSKK18 pKa = 10.9LVGLLPKK25 pKa = 10.47LSSRR29 pKa = 11.84LIDD32 pKa = 3.7AAKK35 pKa = 10.66VPATCGLVVAAGLGLYY51 pKa = 9.93AYY53 pKa = 10.1RR54 pKa = 11.84KK55 pKa = 8.59VCINRR60 pKa = 11.84LEE62 pKa = 4.05FSRR65 pKa = 11.84EE66 pKa = 3.66NTTPRR71 pKa = 11.84PTDD74 pKa = 3.99CIEE77 pKa = 4.52DD78 pKa = 3.6VLEE81 pKa = 4.55AEE83 pKa = 4.55EE84 pKa = 5.37DD85 pKa = 3.84AVTCLALTKK94 pKa = 9.72IAKK97 pKa = 7.77EE98 pKa = 3.9AAMEE102 pKa = 4.17AALNPGEE109 pKa = 3.71ITEE112 pKa = 4.45RR113 pKa = 11.84VRR115 pKa = 11.84EE116 pKa = 3.98AAKK119 pKa = 10.55INAQPSTDD127 pKa = 3.44PLAKK131 pKa = 9.97RR132 pKa = 11.84KK133 pKa = 7.83TRR135 pKa = 11.84LRR137 pKa = 11.84PNKK140 pKa = 8.03TGQFIRR146 pKa = 11.84VLRR149 pKa = 11.84AEE151 pKa = 4.97IKK153 pKa = 10.39SQMGTPTITAANEE166 pKa = 3.42AVIRR170 pKa = 11.84HH171 pKa = 6.13MISKK175 pKa = 9.79FCASHH180 pKa = 6.98NIRR183 pKa = 11.84TTSYY187 pKa = 8.18AHH189 pKa = 5.94LVSRR193 pKa = 11.84VVRR196 pKa = 11.84EE197 pKa = 3.73VMTPYY202 pKa = 10.43PGDD205 pKa = 3.51AEE207 pKa = 4.26EE208 pKa = 4.51VEE210 pKa = 4.53RR211 pKa = 11.84ASSVVNRR218 pKa = 11.84IHH220 pKa = 6.29NWLVQYY226 pKa = 10.53RR227 pKa = 11.84KK228 pKa = 7.98XGGLEE233 pKa = 3.66LARR236 pKa = 11.84GFTHH240 pKa = 6.94EE241 pKa = 4.57AVCTDD246 pKa = 3.52VPGLDD251 pKa = 3.41ATNLGDD257 pKa = 5.06SRR259 pKa = 11.84PLTDD263 pKa = 3.15TNIRR267 pKa = 11.84RR268 pKa = 11.84VVGPVSSDD276 pKa = 3.14YY277 pKa = 11.43DD278 pKa = 3.33IVFYY282 pKa = 10.91TNSRR286 pKa = 11.84NNLMRR291 pKa = 11.84GLVNRR296 pKa = 11.84VLTYY300 pKa = 10.52KK301 pKa = 10.51GGPVLEE307 pKa = 4.79PSPGAWKK314 pKa = 10.36SLRR317 pKa = 11.84GLATSLGNRR326 pKa = 11.84CLTTPLSPDD335 pKa = 3.27EE336 pKa = 5.13FLACYY341 pKa = 9.85VGRR344 pKa = 11.84KK345 pKa = 5.3RR346 pKa = 11.84TIYY349 pKa = 10.63AKK351 pKa = 10.38AIEE354 pKa = 4.35SFKK357 pKa = 10.18TKK359 pKa = 9.89PWDD362 pKa = 3.32ARR364 pKa = 11.84KK365 pKa = 10.8DD366 pKa = 3.69MIVKK370 pKa = 10.39AFIKK374 pKa = 10.37KK375 pKa = 10.08EE376 pKa = 3.61KK377 pKa = 10.38DD378 pKa = 3.15KK379 pKa = 11.5LVDD382 pKa = 3.74EE383 pKa = 4.92SCDD386 pKa = 3.43PRR388 pKa = 11.84IIQPRR393 pKa = 11.84TPRR396 pKa = 11.84FVTRR400 pKa = 11.84FGRR403 pKa = 11.84YY404 pKa = 6.91VRR406 pKa = 11.84AIEE409 pKa = 3.86KK410 pKa = 10.1RR411 pKa = 11.84LYY413 pKa = 10.85SEE415 pKa = 3.79WTKK418 pKa = 10.96SFSSFTVTNTPVCLKK433 pKa = 10.25GMNYY437 pKa = 7.53RR438 pKa = 11.84TRR440 pKa = 11.84ARR442 pKa = 11.84ALLEE446 pKa = 3.78KK447 pKa = 9.17WQSFDD452 pKa = 4.04SPVAVCLDD460 pKa = 3.62ASRR463 pKa = 11.84FDD465 pKa = 3.53LHH467 pKa = 8.5VSVDD471 pKa = 3.41ALKK474 pKa = 9.34FTDD477 pKa = 4.33QIYY480 pKa = 10.76LSAFTGIDD488 pKa = 3.01RR489 pKa = 11.84SEE491 pKa = 4.08LKK493 pKa = 10.36EE494 pKa = 3.77ILRR497 pKa = 11.84NRR499 pKa = 11.84HH500 pKa = 4.92KK501 pKa = 8.66TTGFATFKK509 pKa = 10.46EE510 pKa = 5.06GTFHH514 pKa = 6.14YY515 pKa = 9.25EE516 pKa = 3.81KK517 pKa = 11.08VGGRR521 pKa = 11.84CSGDD525 pKa = 3.03SDD527 pKa = 3.71TSLGNVSIMLAITRR541 pKa = 11.84VICEE545 pKa = 4.1GLKK548 pKa = 10.56GIHH551 pKa = 6.47IEE553 pKa = 4.21VANDD557 pKa = 3.44GDD559 pKa = 4.19DD560 pKa = 3.46QVLMVEE566 pKa = 4.86TNQLDD571 pKa = 3.97NLVKK575 pKa = 10.34EE576 pKa = 4.66LSPTFARR583 pKa = 11.84FGFRR587 pKa = 11.84VKK589 pKa = 10.84VEE591 pKa = 3.99DD592 pKa = 4.01PVWEE596 pKa = 4.24FEE598 pKa = 5.68RR599 pKa = 11.84IDD601 pKa = 3.62FCQTRR606 pKa = 11.84PIFLSPGEE614 pKa = 4.71PIMCRR619 pKa = 11.84YY620 pKa = 9.11PMQSMSKK627 pKa = 10.41DD628 pKa = 3.31VASFLNIEE636 pKa = 4.4RR637 pKa = 11.84SEE639 pKa = 3.9GWKK642 pKa = 10.7YY643 pKa = 8.61MLRR646 pKa = 11.84AIGACGSSSFSQVPVLGEE664 pKa = 4.1FYY666 pKa = 10.72SALSSTSNKK675 pKa = 10.49DD676 pKa = 2.94EE677 pKa = 4.45TKK679 pKa = 7.56WWRR682 pKa = 11.84RR683 pKa = 11.84TGVDD687 pKa = 3.89LGFKK691 pKa = 10.29QLTNNVGTIPYY702 pKa = 10.17DD703 pKa = 3.43EE704 pKa = 4.25VTARR708 pKa = 11.84SSFYY712 pKa = 10.37KK713 pKa = 10.73AFGILPDD720 pKa = 3.65TQAALEE726 pKa = 4.26SKK728 pKa = 9.69IRR730 pKa = 11.84NWNMSSVPHH739 pKa = 6.81IEE741 pKa = 4.01QVHH744 pKa = 5.58KK745 pKa = 10.53FSSEE749 pKa = 4.0FPVVDD754 pKa = 4.35GWAA757 pKa = 3.26

Molecular weight: 84.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O37366|O37366_9VIRU Uncharacterized protein OS=Carrot red leaf luteovirus associated RNA OX=67962 PE=4 SV=1
MM1 pKa = 7.49TNLNIEE7 pKa = 4.62SLVEE11 pKa = 3.87TQPAVSKK18 pKa = 10.9LVGLLPKK25 pKa = 10.47LSSRR29 pKa = 11.84LIDD32 pKa = 3.7AAKK35 pKa = 10.66VPATCGLVVAAGLGLYY51 pKa = 9.93AYY53 pKa = 10.1RR54 pKa = 11.84KK55 pKa = 8.59VCINRR60 pKa = 11.84LEE62 pKa = 4.05FSRR65 pKa = 11.84EE66 pKa = 3.66NTTPRR71 pKa = 11.84PTDD74 pKa = 3.99CIEE77 pKa = 4.52DD78 pKa = 3.6VLEE81 pKa = 4.55AEE83 pKa = 4.55EE84 pKa = 5.37DD85 pKa = 3.84AVTCLALTKK94 pKa = 9.72IAKK97 pKa = 7.77EE98 pKa = 3.9AAMEE102 pKa = 4.17AALNPGEE109 pKa = 3.71ITEE112 pKa = 4.45RR113 pKa = 11.84VRR115 pKa = 11.84EE116 pKa = 3.98AAKK119 pKa = 10.55INAQPSTDD127 pKa = 3.44PLAKK131 pKa = 9.97RR132 pKa = 11.84KK133 pKa = 7.83TRR135 pKa = 11.84LRR137 pKa = 11.84PNKK140 pKa = 8.03TGQFIRR146 pKa = 11.84VLRR149 pKa = 11.84AEE151 pKa = 4.97IKK153 pKa = 10.39SQMGTPTITAANEE166 pKa = 3.42AVIRR170 pKa = 11.84HH171 pKa = 6.13MISKK175 pKa = 9.79FCASHH180 pKa = 6.98NIRR183 pKa = 11.84TTSYY187 pKa = 8.18AHH189 pKa = 5.94LVSRR193 pKa = 11.84VVRR196 pKa = 11.84EE197 pKa = 3.73VMTPYY202 pKa = 10.43PGDD205 pKa = 3.51AEE207 pKa = 4.26EE208 pKa = 4.51VEE210 pKa = 4.53RR211 pKa = 11.84ASSVVNRR218 pKa = 11.84IHH220 pKa = 6.29NWLVQYY226 pKa = 10.79RR227 pKa = 11.84KK228 pKa = 10.18

Molecular weight: 25.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1024

39

757

341.3

38.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.301 ± 1.861

2.051 ± 0.461

4.492 ± 0.806

6.738 ± 1.085

3.516 ± 1.351

5.762 ± 2.789

1.563 ± 0.256

5.371 ± 0.672

5.859 ± 0.19

8.203 ± 0.704

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.051 ± 0.141

4.199 ± 0.425

5.176 ± 1.163

2.051 ± 0.461

7.715 ± 0.607

7.227 ± 0.66

7.324 ± 0.521

8.105 ± 0.292

1.27 ± 0.43

2.93 ± 2.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski