Flavobacterium columnare (strain ATCC 49512 / CIP 103533 / TG 44/87)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium columnare

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8X528|G8X528_FLACA Thioredoxin OS=Flavobacterium columnare (strain ATCC 49512 / CIP 103533 / TG 44/87) OX=1041826 GN=FCOL_10175 PE=3 SV=1
MM1 pKa = 7.39SLDD4 pKa = 3.86IIIKK8 pKa = 8.05ITDD11 pKa = 3.49RR12 pKa = 11.84EE13 pKa = 4.57GVVHH17 pKa = 6.67EE18 pKa = 4.55VQAPTDD24 pKa = 3.44MNMNIMEE31 pKa = 4.67LVRR34 pKa = 11.84VYY36 pKa = 10.78EE37 pKa = 4.17LAPEE41 pKa = 4.22GTIGICGGMAMCASCQCYY59 pKa = 10.14VLNDD63 pKa = 3.38VEE65 pKa = 5.33LPEE68 pKa = 4.82KK69 pKa = 11.09NPDD72 pKa = 3.17EE73 pKa = 5.69DD74 pKa = 4.22AMLWEE79 pKa = 4.39ALNVKK84 pKa = 9.6EE85 pKa = 4.69NSRR88 pKa = 11.84LGCQILITPEE98 pKa = 3.55IDD100 pKa = 3.1GLAIEE105 pKa = 4.98LAPTEE110 pKa = 4.16

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8X9H8|G8X9H8_FLACA Argininosuccinate lyase OS=Flavobacterium columnare (strain ATCC 49512 / CIP 103533 / TG 44/87) OX=1041826 GN=FCOL_08640 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.53HH17 pKa = 4.24GFMEE21 pKa = 4.85RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2617

0

2617

888968

32

4739

339.7

38.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.793 ± 0.052

0.83 ± 0.017

5.042 ± 0.033

6.698 ± 0.064

5.356 ± 0.041

6.044 ± 0.051

1.769 ± 0.026

8.515 ± 0.051

8.646 ± 0.071

9.331 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.027

6.549 ± 0.069

3.313 ± 0.034

3.622 ± 0.028

3.187 ± 0.028

6.237 ± 0.041

5.871 ± 0.075

5.857 ± 0.05

0.968 ± 0.016

4.253 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski