Streptococcus sp. DD12

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; unclassified Streptococcus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1598 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139NL21|A0A139NL21_9STRE NAD kinase OS=Streptococcus sp. DD12 OX=1777880 GN=nadK PE=3 SV=1
MM1 pKa = 7.62ALAKK5 pKa = 9.78IVYY8 pKa = 10.62ASMTGNNEE16 pKa = 3.77EE17 pKa = 4.17IADD20 pKa = 3.64IVAQKK25 pKa = 10.8LEE27 pKa = 4.37EE28 pKa = 4.63LGHH31 pKa = 5.57TVDD34 pKa = 4.3VDD36 pKa = 3.35EE37 pKa = 4.98CTTVEE42 pKa = 4.05PEE44 pKa = 3.95EE45 pKa = 5.17FEE47 pKa = 4.97DD48 pKa = 5.34ADD50 pKa = 3.53ICVVATYY57 pKa = 9.26TYY59 pKa = 11.39GDD61 pKa = 3.61GDD63 pKa = 4.31LPDD66 pKa = 5.1EE67 pKa = 4.45IVDD70 pKa = 4.62FYY72 pKa = 11.83EE73 pKa = 5.1DD74 pKa = 4.37LQDD77 pKa = 5.51LDD79 pKa = 4.9LSDD82 pKa = 5.32HH83 pKa = 6.79IYY85 pKa = 10.57GVCGSGDD92 pKa = 3.1TFYY95 pKa = 11.12DD96 pKa = 3.99YY97 pKa = 11.04FCKK100 pKa = 10.62SVDD103 pKa = 3.58EE104 pKa = 4.3FDD106 pKa = 3.43EE107 pKa = 4.8AFARR111 pKa = 11.84TGATRR116 pKa = 11.84GAEE119 pKa = 4.06KK120 pKa = 10.91VKK122 pKa = 10.86VDD124 pKa = 5.16LSCDD128 pKa = 3.59DD129 pKa = 5.36DD130 pKa = 6.88DD131 pKa = 4.89IANLEE136 pKa = 4.14AFAQALSDD144 pKa = 3.72QVGG147 pKa = 2.98

Molecular weight: 16.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139NJ96|A0A139NJ96_9STRE Putative secretion system component EssB/YukC OS=Streptococcus sp. DD12 OX=1777880 GN=STRDD12_01019 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.73IRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84KK15 pKa = 7.52HH16 pKa = 5.18GFRR19 pKa = 11.84HH20 pKa = 6.46RR21 pKa = 11.84MATKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1598

0

1598

497198

37

4608

311.1

34.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.844 ± 0.075

0.487 ± 0.015

5.863 ± 0.069

6.166 ± 0.064

4.276 ± 0.055

6.834 ± 0.054

1.914 ± 0.034

6.275 ± 0.062

5.708 ± 0.065

10.363 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.027

3.788 ± 0.043

3.497 ± 0.034

4.702 ± 0.056

4.299 ± 0.059

6.607 ± 0.264

6.157 ± 0.103

7.232 ± 0.055

0.959 ± 0.024

3.679 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski