Salicola phage CGphi29

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4R243|M4R243_9CAUD Uncharacterized protein OS=Salicola phage CGphi29 OX=754067 GN=SLPG_00053 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 6.15LLLILILLFSVLVFVVLPLWAICTPSDD29 pKa = 4.48PISEE33 pKa = 4.51YY34 pKa = 10.92EE35 pKa = 5.12LDD37 pKa = 6.3DD38 pKa = 3.69NTCWQMRR45 pKa = 11.84RR46 pKa = 11.84QGYY49 pKa = 9.66NEE51 pKa = 3.89TEE53 pKa = 3.74IQKK56 pKa = 10.25FMEE59 pKa = 4.78DD60 pKa = 2.94VGGHH64 pKa = 5.21YY65 pKa = 8.8EE66 pKa = 4.08PPEE69 pKa = 4.09DD70 pKa = 3.47TT71 pKa = 4.69

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QSE0|M4QSE0_9CAUD Uncharacterized protein OS=Salicola phage CGphi29 OX=754067 GN=SLPG_00029 PE=4 SV=1
MM1 pKa = 7.5LLSTLRR7 pKa = 11.84DD8 pKa = 3.12NSARR12 pKa = 11.84LRR14 pKa = 11.84ALAMAEE20 pKa = 4.18SFAEE24 pKa = 4.59TVARR28 pKa = 11.84WVSEE32 pKa = 3.98AEE34 pKa = 3.99SRR36 pKa = 11.84QLSVVRR42 pKa = 11.84EE43 pKa = 4.23AAQDD47 pKa = 3.51MAVEE51 pKa = 4.41ANTPRR56 pKa = 11.84EE57 pKa = 3.94KK58 pKa = 10.69GGRR61 pKa = 11.84LPVDD65 pKa = 3.36TSFLRR70 pKa = 11.84STLRR74 pKa = 11.84AKK76 pKa = 10.56RR77 pKa = 11.84NNKK80 pKa = 8.78PRR82 pKa = 11.84GKK84 pKa = 10.74GEE86 pKa = 3.84NTGRR90 pKa = 11.84APRR93 pKa = 11.84EE94 pKa = 3.32WDD96 pKa = 3.12AGVIALTLQRR106 pKa = 11.84LQLGDD111 pKa = 3.88RR112 pKa = 11.84LVMGWTANYY121 pKa = 9.96AEE123 pKa = 4.35YY124 pKa = 10.48MEE126 pKa = 4.32ARR128 pKa = 11.84YY129 pKa = 8.86MFMRR133 pKa = 11.84SAAQQWPQFVEE144 pKa = 4.85GAAKK148 pKa = 9.84RR149 pKa = 11.84VYY151 pKa = 10.3RR152 pKa = 11.84RR153 pKa = 11.84ASGG156 pKa = 3.09

Molecular weight: 17.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12490

53

1418

195.2

21.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.816 ± 0.462

1.041 ± 0.182

7.07 ± 0.212

8.038 ± 0.526

2.706 ± 0.179

8.319 ± 0.356

1.761 ± 0.212

4.82 ± 0.228

4.804 ± 0.407

6.926 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.179

3.803 ± 0.167

4.484 ± 0.411

4.444 ± 0.287

6.517 ± 0.322

6.181 ± 0.356

5.821 ± 0.293

6.277 ± 0.284

1.753 ± 0.238

2.938 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski