Beihai tombus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG87|A0A1L3KG87_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 1 OX=1922712 PE=4 SV=1
MM1 pKa = 7.68SRR3 pKa = 11.84GTGFACLLATSEE15 pKa = 4.43CIKK18 pKa = 10.7LDD20 pKa = 3.4VLHH23 pKa = 6.68GVGHH27 pKa = 7.11ISPSLLLDD35 pKa = 3.89LPVEE39 pKa = 4.12SSTIIEE45 pKa = 4.47SVHH48 pKa = 6.79RR49 pKa = 11.84EE50 pKa = 3.85PNYY53 pKa = 11.2AKK55 pKa = 10.6YY56 pKa = 10.62GWFAVGGLAAYY67 pKa = 9.81AGYY70 pKa = 10.23RR71 pKa = 11.84YY72 pKa = 10.0LRR74 pKa = 11.84NRR76 pKa = 11.84YY77 pKa = 7.27SQRR80 pKa = 11.84LNHH83 pKa = 5.27TQYY86 pKa = 11.61DD87 pKa = 3.88KK88 pKa = 11.41LHH90 pKa = 6.62EE91 pKa = 4.36EE92 pKa = 4.21VATLLEE98 pKa = 4.61VNDD101 pKa = 4.27EE102 pKa = 4.27PVDD105 pKa = 3.83PEE107 pKa = 4.99DD108 pKa = 3.99PVEE111 pKa = 4.22TTNEE115 pKa = 4.13DD116 pKa = 3.19GSVEE120 pKa = 4.31VVVRR124 pKa = 11.84RR125 pKa = 11.84PRR127 pKa = 11.84KK128 pKa = 8.48RR129 pKa = 11.84SHH131 pKa = 6.36RR132 pKa = 11.84KK133 pKa = 8.57HH134 pKa = 6.68RR135 pKa = 11.84FITDD139 pKa = 4.52CIHH142 pKa = 4.76MTKK145 pKa = 10.35AHH147 pKa = 6.79FGGCPKK153 pKa = 9.6FTEE156 pKa = 4.49SNQIAVSRR164 pKa = 11.84HH165 pKa = 4.06VYY167 pKa = 9.04EE168 pKa = 3.93LCKK171 pKa = 10.11EE172 pKa = 4.5RR173 pKa = 11.84KK174 pKa = 9.12CLPHH178 pKa = 4.33QTRR181 pKa = 11.84TIMAVAVPAVFSPDD195 pKa = 3.69DD196 pKa = 4.39LDD198 pKa = 3.79VSSAQAYY205 pKa = 9.98NIGLRR210 pKa = 11.84RR211 pKa = 11.84EE212 pKa = 3.98RR213 pKa = 11.84RR214 pKa = 11.84SMVQKK219 pKa = 10.96AMEE222 pKa = 4.47GPRR225 pKa = 11.84WYY227 pKa = 10.06EE228 pKa = 3.71GLLDD232 pKa = 3.94NPLNAVAWRR241 pKa = 11.84EE242 pKa = 3.87AMDD245 pKa = 3.67WLFGLPSWKK254 pKa = 10.28AIRR257 pKa = 11.84AVKK260 pKa = 10.26

Molecular weight: 29.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG87|A0A1L3KG87_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 1 OX=1922712 PE=4 SV=1
MM1 pKa = 7.86AIVPYY6 pKa = 10.35NAAGQLASLITHH18 pKa = 7.16PGIQQKK24 pKa = 7.78VAKK27 pKa = 9.29FASQGLPWASKK38 pKa = 10.23LLSAGWQKK46 pKa = 9.24LTKK49 pKa = 9.82GQRR52 pKa = 11.84KK53 pKa = 6.0QARR56 pKa = 11.84NALNLQPKK64 pKa = 8.51IQANMVPVEE73 pKa = 4.04VGISKK78 pKa = 10.33SAIVKK83 pKa = 9.89GRR85 pKa = 11.84IPRR88 pKa = 11.84YY89 pKa = 8.33KK90 pKa = 10.01QSAGYY95 pKa = 7.96VTISHH100 pKa = 6.7RR101 pKa = 11.84EE102 pKa = 3.84YY103 pKa = 10.44LAEE106 pKa = 4.6AEE108 pKa = 4.69LVNGTDD114 pKa = 2.7IRR116 pKa = 11.84GYY118 pKa = 10.13VLNPSNPRR126 pKa = 11.84AFTWLCTFARR136 pKa = 11.84NFEE139 pKa = 4.23KK140 pKa = 11.0YY141 pKa = 8.89RR142 pKa = 11.84FRR144 pKa = 11.84NIRR147 pKa = 11.84LTYY150 pKa = 9.91VPQAPTTAAGRR161 pKa = 11.84VILAYY166 pKa = 10.47DD167 pKa = 3.91KK168 pKa = 11.18DD169 pKa = 4.01STDD172 pKa = 3.39ILPSGKK178 pKa = 10.19AEE180 pKa = 4.11LYY182 pKa = 10.23SYY184 pKa = 10.63QGTVDD189 pKa = 3.51TPVWQGTSIAFPCDD203 pKa = 2.9NTLRR207 pKa = 11.84FVDD210 pKa = 3.86SSNTAEE216 pKa = 4.12TRR218 pKa = 11.84LVDD221 pKa = 4.15LGQFITCTSAVSAFNGGEE239 pKa = 3.79FFIDD243 pKa = 3.63YY244 pKa = 9.09TVEE247 pKa = 3.71LHH249 pKa = 6.44TPQQPQPMSEE259 pKa = 4.14RR260 pKa = 11.84IYY262 pKa = 10.01TVVGIPAEE270 pKa = 4.08YY271 pKa = 10.19HH272 pKa = 5.74GDD274 pKa = 3.59LMVVEE279 pKa = 5.26SNNSTTEE286 pKa = 4.71FGIEE290 pKa = 3.8WQSVGFYY297 pKa = 10.19HH298 pKa = 7.54VSITINGDD306 pKa = 3.57CSSMATNTHH315 pKa = 6.84DD316 pKa = 3.82DD317 pKa = 3.21AHH319 pKa = 6.49IVRR322 pKa = 11.84TTSSQNGTTSMTIAMFVRR340 pKa = 11.84VVSFDD345 pKa = 3.23PTAPPGILFTLLSGRR360 pKa = 11.84GSVHH364 pKa = 6.14TYY366 pKa = 5.29VTRR369 pKa = 11.84VSSSVAFSS377 pKa = 3.24

Molecular weight: 41.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1142

260

505

380.7

42.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.968 ± 0.254

1.839 ± 0.326

4.553 ± 0.35

5.342 ± 0.797

4.203 ± 0.509

7.093 ± 0.522

2.715 ± 0.507

4.904 ± 0.526

4.729 ± 0.6

7.618 ± 0.566

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.187

4.378 ± 0.314

5.517 ± 0.123

3.678 ± 0.621

6.305 ± 0.764

7.618 ± 0.6

5.342 ± 1.705

8.406 ± 0.078

1.751 ± 0.178

3.765 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski