Amino acid dipepetide frequency for Halonotius sp. J07HN4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.155AlaAla: 15.155 ± 0.191	0.699AlaCys: 0.699 ± 0.032	11.459AlaAsp: 11.459 ± 0.132	8.853AlaGlu: 8.853 ± 0.133	3.506AlaPhe: 3.506 ± 0.073	9.735AlaGly: 9.735 ± 0.123	1.731AlaHis: 1.731 ± 0.043	5.641AlaIle: 5.641 ± 0.102	1.99AlaLys: 1.99 ± 0.063	8.781AlaLeu: 8.781 ± 0.126	2.053AlaMet: 2.053 ± 0.052	2.549AlaAsn: 2.549 ± 0.056	3.549AlaPro: 3.549 ± 0.065	2.194AlaGln: 2.194 ± 0.06	5.228AlaArg: 5.228 ± 0.074	5.116AlaSer: 5.116 ± 0.085	8.262AlaThr: 8.262 ± 0.121	10.982AlaVal: 10.982 ± 0.139	0.989AlaTrp: 0.989 ± 0.035	2.609AlaTyr: 2.609 ± 0.061	0.03AlaXaa: 0.03 ± 0.006
Cys	0.617CysAla: 0.617 ± 0.03	0.117CysCys: 0.117 ± 0.012	0.576CysAsp: 0.576 ± 0.028	0.624CysGlu: 0.624 ± 0.027	0.245CysPhe: 0.245 ± 0.02	0.883CysGly: 0.883 ± 0.036	0.192CysHis: 0.192 ± 0.019	0.264CysIle: 0.264 ± 0.02	0.135CysLys: 0.135 ± 0.014	0.634CysLeu: 0.634 ± 0.026	0.151CysMet: 0.151 ± 0.015	0.228CysAsn: 0.228 ± 0.016	0.528CysPro: 0.528 ± 0.022	0.209CysGln: 0.209 ± 0.016	0.566CysArg: 0.566 ± 0.031	0.52CysSer: 0.52 ± 0.027	0.409CysThr: 0.409 ± 0.022	0.483CysVal: 0.483 ± 0.024	0.118CysTrp: 0.118 ± 0.013	0.201CysTyr: 0.201 ± 0.018	0.001CysXaa: 0.001 ± 0.001
Asp	10.006AspAla: 10.006 ± 0.138	0.767AspCys: 0.767 ± 0.034	8.89AspAsp: 8.89 ± 0.142	7.51AspGlu: 7.51 ± 0.12	1.776AspPhe: 1.776 ± 0.047	7.912AspGly: 7.912 ± 0.104	2.08AspHis: 2.08 ± 0.057	3.907AspIle: 3.907 ± 0.072	1.242AspLys: 1.242 ± 0.043	6.423AspLeu: 6.423 ± 0.107	1.249AspMet: 1.249 ± 0.04	2.156AspAsn: 2.156 ± 0.048	4.36AspPro: 4.36 ± 0.079	2.044AspGln: 2.044 ± 0.05	6.845AspArg: 6.845 ± 0.109	5.039AspSer: 5.039 ± 0.089	5.598AspThr: 5.598 ± 0.086	5.823AspVal: 5.823 ± 0.097	0.852AspTrp: 0.852 ± 0.03	1.787AspTyr: 1.787 ± 0.05	0.015AspXaa: 0.015 ± 0.004
Glu	8.564GluAla: 8.564 ± 0.14	0.541GluCys: 0.541 ± 0.029	4.286GluAsp: 4.286 ± 0.1	5.957GluGlu: 5.957 ± 0.122	2.806GluPhe: 2.806 ± 0.061	4.56GluGly: 4.56 ± 0.083	1.767GluHis: 1.767 ± 0.054	4.638GluIle: 4.638 ± 0.102	1.708GluLys: 1.708 ± 0.049	8.05GluLeu: 8.05 ± 0.131	1.857GluMet: 1.857 ± 0.049	2.088GluAsn: 2.088 ± 0.055	4.281GluPro: 4.281 ± 0.081	3.204GluGln: 3.204 ± 0.066	5.495GluArg: 5.495 ± 0.08	5.258GluSer: 5.258 ± 0.097	7.254GluThr: 7.254 ± 0.104	4.78GluVal: 4.78 ± 0.09	0.969GluTrp: 0.969 ± 0.036	2.388GluTyr: 2.388 ± 0.066	0.015GluXaa: 0.015 ± 0.004
Phe	3.27PheAla: 3.27 ± 0.071	0.256PheCys: 0.256 ± 0.017	3.226PheAsp: 3.226 ± 0.068	2.947PheGlu: 2.947 ± 0.067	0.953PhePhe: 0.953 ± 0.038	3.081PheGly: 3.081 ± 0.076	0.549PheHis: 0.549 ± 0.023	1.33PheIle: 1.33 ± 0.04	0.509PheLys: 0.509 ± 0.022	2.514PheLeu: 2.514 ± 0.068	0.478PheMet: 0.478 ± 0.027	0.739PheAsn: 0.739 ± 0.027	1.188PhePro: 1.188 ± 0.041	0.802PheGln: 0.802 ± 0.032	1.611PheArg: 1.611 ± 0.043	1.729PheSer: 1.729 ± 0.053	1.863PheThr: 1.863 ± 0.045	2.825PheVal: 2.825 ± 0.061	0.329PheTrp: 0.329 ± 0.022	0.813PheTyr: 0.813 ± 0.032	0.004PheXaa: 0.004 ± 0.002
Gly	7.219GlyAla: 7.219 ± 0.124	0.745GlyCys: 0.745 ± 0.03	6.991GlyAsp: 6.991 ± 0.107	6.639GlyGlu: 6.639 ± 0.098	3.007GlyPhe: 3.007 ± 0.069	7.47GlyGly: 7.47 ± 0.135	1.628GlyHis: 1.628 ± 0.047	4.571GlyIle: 4.571 ± 0.076	1.782GlyLys: 1.782 ± 0.048	7.349GlyLeu: 7.349 ± 0.128	1.7GlyMet: 1.7 ± 0.053	1.858GlyAsn: 1.858 ± 0.05	2.839GlyPro: 2.839 ± 0.063	2.248GlyGln: 2.248 ± 0.053	4.689GlyArg: 4.689 ± 0.077	5.127GlySer: 5.127 ± 0.091	5.252GlyThr: 5.252 ± 0.076	7.445GlyVal: 7.445 ± 0.114	1.017GlyTrp: 1.017 ± 0.037	2.645GlyTyr: 2.645 ± 0.062	0.009GlyXaa: 0.009 ± 0.003
His	2.007HisAla: 2.007 ± 0.051	0.193HisCys: 0.193 ± 0.014	1.811HisAsp: 1.811 ± 0.043	1.543HisGlu: 1.543 ± 0.039	0.464HisPhe: 0.464 ± 0.024	1.83HisGly: 1.83 ± 0.052	0.549HisHis: 0.549 ± 0.027	0.941HisIle: 0.941 ± 0.035	0.347HisLys: 0.347 ± 0.018	1.624HisLeu: 1.624 ± 0.047	0.264HisMet: 0.264 ± 0.018	0.569HisAsn: 0.569 ± 0.028	1.13HisPro: 1.13 ± 0.038	0.506HisGln: 0.506 ± 0.024	1.228HisArg: 1.228 ± 0.035	0.941HisSer: 0.941 ± 0.039	1.231HisThr: 1.231 ± 0.038	1.458HisVal: 1.458 ± 0.045	0.227HisTrp: 0.227 ± 0.016	0.5HisTyr: 0.5 ± 0.027	0.002HisXaa: 0.002 ± 0.002
Ile	5.694IleAla: 5.694 ± 0.083	0.339IleCys: 0.339 ± 0.017	5.286IleAsp: 5.286 ± 0.084	4.952IleGlu: 4.952 ± 0.092	1.102IlePhe: 1.102 ± 0.043	4.391IleGly: 4.391 ± 0.087	0.919IleHis: 0.919 ± 0.032	1.947IleIle: 1.947 ± 0.063	0.999IleLys: 0.999 ± 0.038	3.232IleLeu: 3.232 ± 0.077	0.552IleMet: 0.552 ± 0.021	1.383IleAsn: 1.383 ± 0.046	2.267IlePro: 2.267 ± 0.053	1.321IleGln: 1.321 ± 0.04	2.681IleArg: 2.681 ± 0.062	2.565IleSer: 2.565 ± 0.066	3.189IleThr: 3.189 ± 0.071	4.109IleVal: 4.109 ± 0.084	0.342IleTrp: 0.342 ± 0.02	0.979IleTyr: 0.979 ± 0.038	0.009IleXaa: 0.009 ± 0.003
Lys	1.867LysAla: 1.867 ± 0.053	0.116LysCys: 0.116 ± 0.013	0.99LysAsp: 0.99 ± 0.042	1.362LysGlu: 1.362 ± 0.047	0.501LysPhe: 0.501 ± 0.025	1.345LysGly: 1.345 ± 0.047	0.477LysHis: 0.477 ± 0.021	0.951LysIle: 0.951 ± 0.037	0.527LysLys: 0.527 ± 0.037	1.877LysLeu: 1.877 ± 0.047	0.376LysMet: 0.376 ± 0.021	0.555LysAsn: 0.555 ± 0.023	1.074LysPro: 1.074 ± 0.04	1.086LysGln: 1.086 ± 0.048	1.667LysArg: 1.667 ± 0.046	1.194LysSer: 1.194 ± 0.037	1.511LysThr: 1.511 ± 0.05	1.033LysVal: 1.033 ± 0.029	0.219LysTrp: 0.219 ± 0.015	0.497LysTyr: 0.497 ± 0.023	0.002LysXaa: 0.002 ± 0.001
Leu	10.438LeuAla: 10.438 ± 0.143	0.629LeuCys: 0.629 ± 0.027	7.384LeuAsp: 7.384 ± 0.117	5.455LeuGlu: 5.455 ± 0.108	2.996LeuPhe: 2.996 ± 0.073	7.219LeuGly: 7.219 ± 0.104	1.35LeuHis: 1.35 ± 0.037	3.794LeuIle: 3.794 ± 0.07	1.636LeuLys: 1.636 ± 0.052	8.692LeuLeu: 8.692 ± 0.16	1.409LeuMet: 1.409 ± 0.048	2.034LeuAsn: 2.034 ± 0.056	4.196LeuPro: 4.196 ± 0.078	2.399LeuGln: 2.399 ± 0.058	5.367LeuArg: 5.367 ± 0.079	5.893LeuSer: 5.893 ± 0.092	6.282LeuThr: 6.282 ± 0.092	7.136LeuVal: 7.136 ± 0.114	0.895LeuTrp: 0.895 ± 0.039	2.077LeuTyr: 2.077 ± 0.05	0.016LeuXaa: 0.016 ± 0.004
Met	1.863MetAla: 1.863 ± 0.043	0.123MetCys: 0.123 ± 0.013	1.177MetAsp: 1.177 ± 0.042	1.103MetGlu: 1.103 ± 0.039	0.49MetPhe: 0.49 ± 0.026	1.336MetGly: 1.336 ± 0.047	0.313MetHis: 0.313 ± 0.02	0.848MetIle: 0.848 ± 0.035	0.44MetLys: 0.44 ± 0.023	1.587MetLeu: 1.587 ± 0.05	0.336MetMet: 0.336 ± 0.024	0.571MetAsn: 0.571 ± 0.028	0.829MetPro: 0.829 ± 0.03	0.533MetGln: 0.533 ± 0.029	0.968MetArg: 0.968 ± 0.029	1.705MetSer: 1.705 ± 0.046	1.583MetThr: 1.583 ± 0.042	1.303MetVal: 1.303 ± 0.045	0.159MetTrp: 0.159 ± 0.013	0.353MetTyr: 0.353 ± 0.019	0.004MetXaa: 0.004 ± 0.002
Asn	2.822AsnAla: 2.822 ± 0.063	0.24AsnCys: 0.24 ± 0.017	2.027AsnAsp: 2.027 ± 0.053	1.984AsnGlu: 1.984 ± 0.05	0.677AsnPhe: 0.677 ± 0.028	2.126AsnGly: 2.126 ± 0.055	0.534AsnHis: 0.534 ± 0.023	1.113AsnIle: 1.113 ± 0.041	0.548AsnLys: 0.548 ± 0.028	1.927AsnLeu: 1.927 ± 0.047	0.475AsnMet: 0.475 ± 0.026	0.793AsnAsn: 0.793 ± 0.033	1.645AsnPro: 1.645 ± 0.049	0.697AsnGln: 0.697 ± 0.029	1.78AsnArg: 1.78 ± 0.043	1.304AsnSer: 1.304 ± 0.043	1.703AsnThr: 1.703 ± 0.05	1.965AsnVal: 1.965 ± 0.052	0.272AsnTrp: 0.272 ± 0.022	0.693AsnTyr: 0.693 ± 0.034	0.004AsnXaa: 0.004 ± 0.002
Pro	5.125ProAla: 5.125 ± 0.098	0.281ProCys: 0.281 ± 0.018	4.572ProAsp: 4.572 ± 0.076	4.223ProGlu: 4.223 ± 0.072	1.471ProPhe: 1.471 ± 0.047	3.401ProGly: 3.401 ± 0.065	0.787ProHis: 0.787 ± 0.032	2.173ProIle: 2.173 ± 0.052	0.858ProLys: 0.858 ± 0.037	3.699ProLeu: 3.699 ± 0.069	0.808ProMet: 0.808 ± 0.032	1.201ProAsn: 1.201 ± 0.04	2.271ProPro: 2.271 ± 0.057	1.175ProGln: 1.175 ± 0.04	2.186ProArg: 2.186 ± 0.051	2.493ProSer: 2.493 ± 0.058	3.749ProThr: 3.749 ± 0.074	3.86ProVal: 3.86 ± 0.066	0.48ProTrp: 0.48 ± 0.025	1.02ProTyr: 1.02 ± 0.036	0.01ProXaa: 0.01 ± 0.003
Gln	2.916GlnAla: 2.916 ± 0.061	0.229GlnCys: 0.229 ± 0.018	1.358GlnAsp: 1.358 ± 0.045	1.851GlnGlu: 1.851 ± 0.053	1.066GlnPhe: 1.066 ± 0.033	1.632GlnGly: 1.632 ± 0.045	0.67GlnHis: 0.67 ± 0.029	1.433GlnIle: 1.433 ± 0.047	0.629GlnLys: 0.629 ± 0.026	3.059GlnLeu: 3.059 ± 0.064	0.575GlnMet: 0.575 ± 0.029	0.719GlnAsn: 0.719 ± 0.025	1.641GlnPro: 1.641 ± 0.048	1.661GlnGln: 1.661 ± 0.06	2.325GlnArg: 2.325 ± 0.054	1.798GlnSer: 1.798 ± 0.052	2.141GlnThr: 2.141 ± 0.062	1.824GlnVal: 1.824 ± 0.052	0.345GlnTrp: 0.345 ± 0.02	0.883GlnTyr: 0.883 ± 0.035	0.004GlnXaa: 0.004 ± 0.002
Arg	5.856ArgAla: 5.856 ± 0.087	0.568ArgCys: 0.568 ± 0.029	4.026ArgAsp: 4.026 ± 0.076	5.768ArgGlu: 5.768 ± 0.101	2.214ArgPhe: 2.214 ± 0.048	4.125ArgGly: 4.125 ± 0.071	1.283ArgHis: 1.283 ± 0.038	2.966ArgIle: 2.966 ± 0.067	1.411ArgLys: 1.411 ± 0.042	5.995ArgLeu: 5.995 ± 0.093	1.202ArgMet: 1.202 ± 0.037	1.686ArgAsn: 1.686 ± 0.048	2.401ArgPro: 2.401 ± 0.056	2.192ArgGln: 2.192 ± 0.056	4.45ArgArg: 4.45 ± 0.087	3.451ArgSer: 3.451 ± 0.064	3.454ArgThr: 3.454 ± 0.065	4.796ArgVal: 4.796 ± 0.089	0.765ArgTrp: 0.765 ± 0.027	1.809ArgTyr: 1.809 ± 0.047	0.014ArgXaa: 0.014 ± 0.004
Ser	5.367SerAla: 5.367 ± 0.078	0.362SerCys: 0.362 ± 0.024	4.658SerAsp: 4.658 ± 0.072	4.524SerGlu: 4.524 ± 0.084	1.865SerPhe: 1.865 ± 0.05	5.031SerGly: 5.031 ± 0.074	1.15SerHis: 1.15 ± 0.038	2.802SerIle: 2.802 ± 0.062	1.228SerLys: 1.228 ± 0.043	5.072SerLeu: 5.072 ± 0.074	1.122SerMet: 1.122 ± 0.037	1.404SerAsn: 1.404 ± 0.043	2.719SerPro: 2.719 ± 0.055	1.662SerGln: 1.662 ± 0.05	3.2SerArg: 3.2 ± 0.068	3.077SerSer: 3.077 ± 0.082	4.104SerThr: 4.104 ± 0.085	5.286SerVal: 5.286 ± 0.085	0.561SerTrp: 0.561 ± 0.026	1.538SerTyr: 1.538 ± 0.04	0.022SerXaa: 0.022 ± 0.005
Thr	9.069ThrAla: 9.069 ± 0.13	0.398ThrCys: 0.398 ± 0.024	6.689ThrAsp: 6.689 ± 0.104	5.332ThrGlu: 5.332 ± 0.097	1.978ThrPhe: 1.978 ± 0.044	5.889ThrGly: 5.889 ± 0.093	1.295ThrHis: 1.295 ± 0.04	3.552ThrIle: 3.552 ± 0.076	1.183ThrLys: 1.183 ± 0.037	6.298ThrLeu: 6.298 ± 0.097	1.123ThrMet: 1.123 ± 0.037	1.783ThrAsn: 1.783 ± 0.053	3.693ThrPro: 3.693 ± 0.074	1.916ThrGln: 1.916 ± 0.048	3.247ThrArg: 3.247 ± 0.067	2.82ThrSer: 2.82 ± 0.06	5.615ThrThr: 5.615 ± 0.093	7.53ThrVal: 7.53 ± 0.104	0.566ThrTrp: 0.566 ± 0.031	1.544ThrTyr: 1.544 ± 0.043	0.007ThrXaa: 0.007 ± 0.003
Val	9.79ValAla: 9.79 ± 0.125	0.719ValCys: 0.719 ± 0.028	7.884ValAsp: 7.884 ± 0.111	6.74ValGlu: 6.74 ± 0.105	2.736ValPhe: 2.736 ± 0.065	7.451ValGly: 7.451 ± 0.107	1.316ValHis: 1.316 ± 0.043	3.896ValIle: 3.896 ± 0.073	1.364ValLys: 1.364 ± 0.04	6.862ValLeu: 6.862 ± 0.106	1.286ValMet: 1.286 ± 0.038	1.976ValAsn: 1.976 ± 0.058	3.587ValPro: 3.587 ± 0.064	1.709ValGln: 1.709 ± 0.043	4.35ValArg: 4.35 ± 0.076	4.965ValSer: 4.965 ± 0.085	6.031ValThr: 6.031 ± 0.092	8.467ValVal: 8.467 ± 0.128	0.719ValTrp: 0.719 ± 0.03	1.927ValTyr: 1.927 ± 0.05	0.014ValXaa: 0.014 ± 0.004
Trp	0.938TrpAla: 0.938 ± 0.033	0.103TrpCys: 0.103 ± 0.012	0.616TrpAsp: 0.616 ± 0.026	0.802TrpGlu: 0.802 ± 0.031	0.4TrpPhe: 0.4 ± 0.024	0.709TrpGly: 0.709 ± 0.03	0.207TrpHis: 0.207 ± 0.014	0.51TrpIle: 0.51 ± 0.026	0.231TrpLys: 0.231 ± 0.018	1.23TrpLeu: 1.23 ± 0.04	0.211TrpMet: 0.211 ± 0.017	0.321TrpAsn: 0.321 ± 0.019	0.396TrpPro: 0.396 ± 0.023	0.409TrpGln: 0.409 ± 0.019	0.767TrpArg: 0.767 ± 0.033	0.47TrpSer: 0.47 ± 0.027	0.672TrpThr: 0.672 ± 0.028	0.744TrpVal: 0.744 ± 0.032	0.161TrpTrp: 0.161 ± 0.015	0.372TrpTyr: 0.372 ± 0.021	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.509TyrAla: 2.509 ± 0.054	0.275TyrCys: 0.275 ± 0.02	2.365TyrAsp: 2.365 ± 0.055	2.294TyrGlu: 2.294 ± 0.055	0.755TyrPhe: 0.755 ± 0.029	2.09TyrGly: 2.09 ± 0.052	0.56TyrHis: 0.56 ± 0.025	0.97TyrIle: 0.97 ± 0.037	0.456TyrLys: 0.456 ± 0.024	2.381TyrLeu: 2.381 ± 0.067	0.394TyrMet: 0.394 ± 0.02	0.712TyrAsn: 0.712 ± 0.033	1.237TyrPro: 1.237 ± 0.038	0.787TyrGln: 0.787 ± 0.036	1.753TyrArg: 1.753 ± 0.053	1.278TyrSer: 1.278 ± 0.039	1.612TyrThr: 1.612 ± 0.046	1.927TyrVal: 1.927 ± 0.05	0.283TyrTrp: 0.283 ± 0.02	0.766TyrTyr: 0.766 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.017XaaAla: 0.017 ± 0.005	0.002XaaCys: 0.002 ± 0.002	0.006XaaAsp: 0.006 ± 0.003	0.004XaaGlu: 0.004 ± 0.002	0.005XaaPhe: 0.005 ± 0.002	0.016XaaGly: 0.016 ± 0.005	0.009XaaHis: 0.009 ± 0.003	0.009XaaIle: 0.009 ± 0.004	0.002XaaLys: 0.002 ± 0.002	0.011XaaLeu: 0.011 ± 0.004	0.006XaaMet: 0.006 ± 0.003	0.006XaaAsn: 0.006 ± 0.003	0.01XaaPro: 0.01 ± 0.003	0.004XaaGln: 0.004 ± 0.002	0.027XaaArg: 0.027 ± 0.007	0.012XaaSer: 0.012 ± 0.004	0.011XaaThr: 0.011 ± 0.004	0.016XaaVal: 0.016 ± 0.004	0.002XaaTrp: 0.002 ± 0.002	0.005XaaTyr: 0.005 ± 0.002	1.141XaaXaa: 1.141 ± 0.096

Statistics based on 3224 proteins (812135 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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