Emilia yellow vein virus-[Fz1]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Emilia yellow vein virus

Average proteome isoelectric point is 8.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0LV70|B0LV70_9GEMI Capsid protein OS=Emilia yellow vein virus-[Fz1] OX=498805 GN=AV1 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.84FPDD11 pKa = 4.03TVHH14 pKa = 7.15GFRR17 pKa = 11.84CMLANKK23 pKa = 9.78YY24 pKa = 9.2LLCIADD30 pKa = 4.06TYY32 pKa = 11.79SPDD35 pKa = 3.23TLGYY39 pKa = 10.84DD40 pKa = 4.83LIRR43 pKa = 11.84DD44 pKa = 4.71LISVIRR50 pKa = 11.84ARR52 pKa = 11.84DD53 pKa = 3.41YY54 pKa = 11.52GEE56 pKa = 3.45ATRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 8.49SHH63 pKa = 6.04FHH65 pKa = 5.64SRR67 pKa = 11.84FEE69 pKa = 4.33GTSTTEE75 pKa = 3.56LRR77 pKa = 11.84QPIYY81 pKa = 10.77SPCCCPHH88 pKa = 6.85CPRR91 pKa = 11.84HH92 pKa = 5.77QKK94 pKa = 9.37TDD96 pKa = 3.11MDD98 pKa = 3.99QQAHH102 pKa = 4.79VSKK105 pKa = 10.71AQNLQNDD112 pKa = 4.18KK113 pKa = 11.19KK114 pKa = 10.56PP115 pKa = 3.37

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0LV71|B0LV71_9GEMI Replication enhancer OS=Emilia yellow vein virus-[Fz1] OX=498805 GN=AC3 PE=3 SV=1
MM1 pKa = 7.9AKK3 pKa = 10.07RR4 pKa = 11.84PGDD7 pKa = 3.65IVISTPVSKK16 pKa = 10.55VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.01SPYY27 pKa = 8.62TRR29 pKa = 11.84RR30 pKa = 11.84AAVPTVRR37 pKa = 11.84VTRR40 pKa = 11.84RR41 pKa = 11.84QIWTNRR47 pKa = 11.84PMYY50 pKa = 9.69RR51 pKa = 11.84KK52 pKa = 9.23PKK54 pKa = 8.48IYY56 pKa = 10.57RR57 pKa = 11.84MTRR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.21VQSFEE78 pKa = 3.84QRR80 pKa = 11.84HH81 pKa = 6.27DD82 pKa = 3.18IAHH85 pKa = 6.13TGKK88 pKa = 10.47VICISDD94 pKa = 3.37ITRR97 pKa = 11.84GNGLTHH103 pKa = 6.89RR104 pKa = 11.84VGKK107 pKa = 9.89RR108 pKa = 11.84FCIKK112 pKa = 9.25STYY115 pKa = 10.18ILGKK119 pKa = 9.73VWMDD123 pKa = 3.27EE124 pKa = 4.06NIKK127 pKa = 9.31TKK129 pKa = 10.61NHH131 pKa = 5.92TNTVMFYY138 pKa = 9.52VVRR141 pKa = 11.84DD142 pKa = 3.7RR143 pKa = 11.84RR144 pKa = 11.84PFGTPMDD151 pKa = 4.58FGQVFNMYY159 pKa = 10.66DD160 pKa = 3.86NEE162 pKa = 4.36PSTATIKK169 pKa = 10.89NDD171 pKa = 3.0LRR173 pKa = 11.84DD174 pKa = 3.56RR175 pKa = 11.84YY176 pKa = 9.95QVLRR180 pKa = 11.84KK181 pKa = 9.66FWATVTGGQYY191 pKa = 10.85ASKK194 pKa = 9.08EE195 pKa = 3.85QAMVAKK201 pKa = 10.32FMKK204 pKa = 10.16LNNYY208 pKa = 8.37VCYY211 pKa = 10.34NHH213 pKa = 6.35QEE215 pKa = 3.65AAKK218 pKa = 10.4YY219 pKa = 9.25EE220 pKa = 4.13NHH222 pKa = 6.46TEE224 pKa = 3.99NALLLYY230 pKa = 7.29MACTHH235 pKa = 7.07ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.33IRR247 pKa = 11.84IYY249 pKa = 11.47SMIRR253 pKa = 11.84YY254 pKa = 8.4QINKK258 pKa = 8.72NN259 pKa = 3.31

Molecular weight: 30.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1070

99

328

178.3

20.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.327 ± 0.357

2.336 ± 0.442

4.953 ± 0.683

3.738 ± 0.246

4.112 ± 0.539

4.86 ± 0.532

3.832 ± 0.464

7.196 ± 0.66

7.009 ± 0.468

7.103 ± 0.933

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.804 ± 0.818

5.888 ± 0.79

6.075 ± 0.614

4.393 ± 0.618

6.822 ± 0.951

7.57 ± 1.269

6.075 ± 0.812

4.579 ± 0.974

1.215 ± 0.138

4.112 ± 0.592
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski