Pseudomonas kirkiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3666 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9RGK9|A0A4Q9RGK9_9PSED 50S ribosomal protein L35 OS=Pseudomonas kirkiae OX=2211392 GN=rpmI PE=3 SV=1
MM1 pKa = 7.51SFNTALSGLKK11 pKa = 10.05AASTYY16 pKa = 11.37LNVTGNNIANTGTTGFKK33 pKa = 10.26YY34 pKa = 10.64SRR36 pKa = 11.84AEE38 pKa = 3.81FADD41 pKa = 4.99LYY43 pKa = 10.02ASSMFGTGSNSVGSGVLTSDD63 pKa = 3.48IAQQFTQGNITYY75 pKa = 9.19TDD77 pKa = 3.27NSLDD81 pKa = 3.66LAINGSGYY89 pKa = 10.6FIVNDD94 pKa = 4.64GGSQLYY100 pKa = 8.44TRR102 pKa = 11.84SGAFKK107 pKa = 10.66VDD109 pKa = 3.31NQGNVVDD116 pKa = 4.28SSGNNLMGYY125 pKa = 10.2GVDD128 pKa = 3.7SDD130 pKa = 4.32GNLVNGVLTNLVVDD144 pKa = 4.34TTNQEE149 pKa = 4.17PNATTSVTQSLALNSAASLPTTTPFDD175 pKa = 3.96SSISTSYY182 pKa = 10.66NWSTSATLYY191 pKa = 10.59DD192 pKa = 3.99SQGNAHH198 pKa = 6.07TMSQYY203 pKa = 10.17FVKK206 pKa = 10.01TDD208 pKa = 3.31SNEE211 pKa = 2.98WSMYY215 pKa = 9.69VLVDD219 pKa = 3.23GRR221 pKa = 11.84NPQDD225 pKa = 3.49PTSTDD230 pKa = 3.25PYY232 pKa = 9.87EE233 pKa = 4.51ASLPFTTAGKK243 pKa = 10.55LDD245 pKa = 3.68TSNLASGDD253 pKa = 3.5FTINADD259 pKa = 3.63GTLSLSDD266 pKa = 3.63WVPAAISDD274 pKa = 3.81ATTDD278 pKa = 3.66PVTWASNGAAASTSGITLDD297 pKa = 4.58LRR299 pKa = 11.84TMTQTNTSFSVNSLYY314 pKa = 10.45QDD316 pKa = 3.97GYY318 pKa = 8.16TTGQLSGLSVDD329 pKa = 3.5EE330 pKa = 4.39TGQLFATYY338 pKa = 10.14TNGQSKK344 pKa = 10.98VIGQTALATFANMQGLTPVGGTAWRR369 pKa = 11.84QSLASGEE376 pKa = 4.24PVIGTPGSGTLGAIAAGALEE396 pKa = 4.81DD397 pKa = 4.35SNVDD401 pKa = 3.56LTSQLVDD408 pKa = 4.98LIVAQRR414 pKa = 11.84NYY416 pKa = 9.59QANAKK421 pKa = 9.2TIEE424 pKa = 4.16TEE426 pKa = 3.93NTISQTIINIRR437 pKa = 3.35

Molecular weight: 45.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9REN9|A0A4Q9REN9_9PSED Methyl-accepting chemotaxis protein OS=Pseudomonas kirkiae OX=2211392 GN=DNJ96_03035 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3666

0

3666

1248603

23

6256

340.6

37.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.109 ± 0.056

0.925 ± 0.012

5.432 ± 0.035

6.056 ± 0.046

3.45 ± 0.026

8.291 ± 0.053

2.281 ± 0.02

4.691 ± 0.028

2.813 ± 0.04

12.302 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.015 ± 0.018

2.848 ± 0.032

4.735 ± 0.031

4.646 ± 0.035

7.281 ± 0.049

5.976 ± 0.049

4.51 ± 0.043

6.706 ± 0.04

1.402 ± 0.018

2.533 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski