Lentzea sp. FXJ1.1311

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Lentzea; unclassified Lentzea

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8306 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A563EGI6|A0A563EGI6_9PSEU Gmad1 domain-containing protein OS=Lentzea sp. FXJ1.1311 OX=2591470 GN=FKR81_42100 PE=4 SV=1
MM1 pKa = 7.51FKK3 pKa = 9.74TAAAVCAAAALSLAASPLAHH23 pKa = 7.02ASDD26 pKa = 4.18EE27 pKa = 4.16PSPVSIDD34 pKa = 3.54LDD36 pKa = 3.5RR37 pKa = 11.84GMGMGVDD44 pKa = 5.28RR45 pKa = 11.84EE46 pKa = 4.72GTWTANTFGYY56 pKa = 8.1FTVDD60 pKa = 3.14LSDD63 pKa = 4.25AADD66 pKa = 3.67EE67 pKa = 4.52NLDD70 pKa = 3.72EE71 pKa = 4.61VSVFVSVPDD80 pKa = 3.62AVEE83 pKa = 3.8VADD86 pKa = 4.7YY87 pKa = 11.43SGDD90 pKa = 4.92DD91 pKa = 3.5WDD93 pKa = 4.42CWDD96 pKa = 3.23VDD98 pKa = 4.81GGIEE102 pKa = 4.16CHH104 pKa = 6.23NPQLMVPGEE113 pKa = 4.28AWPTLTVHH121 pKa = 6.93LYY123 pKa = 10.24VHH125 pKa = 6.99DD126 pKa = 4.47HH127 pKa = 6.32MDD129 pKa = 3.86DD130 pKa = 3.37VGSIDD135 pKa = 3.81VYY137 pKa = 11.09ATTGDD142 pKa = 3.88FGPAHH147 pKa = 6.78EE148 pKa = 4.97GVPFQTNTRR157 pKa = 11.84VV158 pKa = 2.97

Molecular weight: 16.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A563EZ03|A0A563EZ03_9PSEU DUF2029 domain-containing protein OS=Lentzea sp. FXJ1.1311 OX=2591470 GN=FKR81_07235 PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.37GRR42 pKa = 11.84ARR44 pKa = 11.84LSAA47 pKa = 3.91

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8306

0

8306

2773018

26

7696

333.9

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.574 ± 0.037

0.857 ± 0.008

6.105 ± 0.023

5.555 ± 0.024

2.99 ± 0.014

8.849 ± 0.027

2.334 ± 0.014

3.456 ± 0.019

2.334 ± 0.024

10.491 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.771 ± 0.011

2.149 ± 0.018

5.645 ± 0.028

2.948 ± 0.014

7.659 ± 0.032

5.446 ± 0.02

5.992 ± 0.026

9.27 ± 0.025

1.593 ± 0.01

1.983 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski