Tardiphaga sp. vice352

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Tardiphaga; unclassified Tardiphaga

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5026 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A515KMV7|A0A515KMV7_9BRAD Peptidase OS=Tardiphaga sp. vice352 OX=2592816 GN=FNL55_18430 PE=4 SV=1
MM1 pKa = 6.49TTADD5 pKa = 3.63TSQSKK10 pKa = 9.66YY11 pKa = 10.73AGPVWRR17 pKa = 11.84GVDD20 pKa = 4.13YY21 pKa = 11.33SPTWTAWVVGAGATQTGDD39 pKa = 3.05SDD41 pKa = 3.85FANDD45 pKa = 3.79AFQSLWAANFMAAPGGDD62 pKa = 3.14TSLPTDD68 pKa = 3.31NGNNYY73 pKa = 10.09RR74 pKa = 11.84NDD76 pKa = 3.51LQIIADD82 pKa = 3.68AGLNLVRR89 pKa = 11.84LYY91 pKa = 10.65NWDD94 pKa = 3.41MARR97 pKa = 11.84GTTATSNVGLDD108 pKa = 3.87HH109 pKa = 7.75INFLDD114 pKa = 3.67AAAGFGLKK122 pKa = 10.08VVVPVSDD129 pKa = 3.6WFLSDD134 pKa = 6.19DD135 pKa = 3.72PNAWNFTPAPLPSNYY150 pKa = 10.29DD151 pKa = 3.34FANAPAAIQTDD162 pKa = 4.2FSQFVASVTDD172 pKa = 3.56PTTGKK177 pKa = 9.14IHH179 pKa = 6.3SAVHH183 pKa = 6.32SIDD186 pKa = 3.32VGNEE190 pKa = 3.23GDD192 pKa = 4.14IGQGLNGQTTPSSFLYY208 pKa = 8.76RR209 pKa = 11.84TIWWIVNLHH218 pKa = 4.56QQINGGSPGPDD229 pKa = 2.76GSPAVNGATPVVPSTATFSNGDD251 pKa = 3.17QGLGIGSWFNCLIAGVAANQQTPTEE276 pKa = 4.21MDD278 pKa = 3.13GHH280 pKa = 6.98SSFDD284 pKa = 3.5VAVTGLKK291 pKa = 10.45AADD294 pKa = 4.04PAWEE298 pKa = 3.82QYY300 pKa = 10.64YY301 pKa = 10.93YY302 pKa = 11.34NSTNISQVSTATPFGNTLAATLALYY327 pKa = 10.56DD328 pKa = 4.29SGASPWPGADD338 pKa = 3.11CTVPLLLMEE347 pKa = 5.55LFTPNRR353 pKa = 11.84TAFPEE358 pKa = 4.29PADD361 pKa = 3.55QAVAAVGQVTDD372 pKa = 4.27LEE374 pKa = 4.83SYY376 pKa = 10.25LAQNSAGTASSSTYY390 pKa = 11.02LMGYY394 pKa = 10.38NYY396 pKa = 10.46FEE398 pKa = 5.28FNDD401 pKa = 3.73EE402 pKa = 4.18QQVKK406 pKa = 8.36LTGLYY411 pKa = 10.17QYY413 pKa = 11.26GATFNPAATGTTSVFYY429 pKa = 10.74SPYY432 pKa = 9.63QFPIMAFPVYY442 pKa = 10.57SLVATPGPNGSGTLIDD458 pKa = 5.54ALTQCVPGNIVAVFGEE474 pKa = 4.38TGNWQATFYY483 pKa = 9.68TGAPIPSWVTTGMSVWGANIPASTTVNVPTPGPGAVDD520 pKa = 3.23MTVVLVCAGSQASNPFTVEE539 pKa = 3.83TVTLSFYY546 pKa = 11.47

Molecular weight: 57.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A515KQU1|A0A515KQU1_9BRAD 5'-methylthioadenosine nucleosidase OS=Tardiphaga sp. vice352 OX=2592816 GN=FNL55_24465 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5026

0

5026

1561869

26

3211

310.8

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.515 ± 0.045

0.827 ± 0.009

5.67 ± 0.027

5.155 ± 0.03

3.751 ± 0.022

8.321 ± 0.033

1.996 ± 0.017

5.495 ± 0.023

3.737 ± 0.035

9.856 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.018

2.754 ± 0.019

5.194 ± 0.031

3.207 ± 0.017

6.766 ± 0.036

5.645 ± 0.024

5.479 ± 0.024

7.468 ± 0.025

1.311 ± 0.015

2.247 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski