Naumovozyma castellii (strain ATCC 76901 / BCRC 22586 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Naumovozyma; Naumovozyma castellii

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0VB11|G0VB11_NAUCC tRNA dimethylallyltransferase OS=Naumovozyma castellii (strain ATCC 76901 / BCRC 22586 / CBS 4309 / NBRC 1992 / NRRL Y-12630) OX=1064592 GN=NCAS0B00500 PE=3 SV=1
MM1 pKa = 7.69EE2 pKa = 4.81SFGFIVKK9 pKa = 8.03TQNDD13 pKa = 3.8LAAVDD18 pKa = 4.09DD19 pKa = 5.48AFVAWRR25 pKa = 11.84DD26 pKa = 3.63NKK28 pKa = 10.25PFSSVAQSVLVDD40 pKa = 3.52TEE42 pKa = 4.46DD43 pKa = 3.28FCGIVVDD50 pKa = 4.19SPKK53 pKa = 10.48VVNQTILSLTTCDD66 pKa = 5.63IIDD69 pKa = 4.26SDD71 pKa = 4.7NEE73 pKa = 4.25TLNEE77 pKa = 4.0FQLSISLLGEE87 pKa = 3.99KK88 pKa = 10.57EE89 pKa = 4.39EE90 pKa = 5.11IPASLNDD97 pKa = 4.51DD98 pKa = 3.95FQDD101 pKa = 3.96LLNNYY106 pKa = 9.1YY107 pKa = 10.52EE108 pKa = 4.45FDD110 pKa = 4.05GVHH113 pKa = 6.44HH114 pKa = 6.78SSANDD119 pKa = 3.77SIVFGAWYY127 pKa = 9.76GSDD130 pKa = 3.25VDD132 pKa = 4.21KK133 pKa = 11.47VILIRR138 pKa = 11.84LSTYY142 pKa = 7.7MWSFTISNGVYY153 pKa = 8.09ANNYY157 pKa = 8.27IEE159 pKa = 5.61HH160 pKa = 6.98IGDD163 pKa = 3.56SAADD167 pKa = 3.34SFGFILNVNNDD178 pKa = 3.69TQLVDD183 pKa = 3.4YY184 pKa = 10.72AISKK188 pKa = 8.88WEE190 pKa = 3.75NHH192 pKa = 4.84EE193 pKa = 4.24TFNDD197 pKa = 3.51NANVIAIDD205 pKa = 3.77DD206 pKa = 4.1EE207 pKa = 5.22YY208 pKa = 11.54YY209 pKa = 10.91CYY211 pKa = 10.56II212 pKa = 4.45

Molecular weight: 23.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0VG32|G0VG32_NAUCC Mitochondrial pyruvate carrier OS=Naumovozyma castellii (strain ATCC 76901 / BCRC 22586 / CBS 4309 / NBRC 1992 / NRRL Y-12630) OX=1064592 GN=NCAS0E03810 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.11WRR6 pKa = 11.84KK7 pKa = 9.07KK8 pKa = 6.93RR9 pKa = 11.84TRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.48RR15 pKa = 11.84KK16 pKa = 8.41RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5565

0

5565

2764192

25

4934

496.7

56.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.197 ± 0.027

1.17 ± 0.011

5.955 ± 0.025

6.736 ± 0.035

4.393 ± 0.023

4.852 ± 0.027

2.089 ± 0.012

6.758 ± 0.026

7.302 ± 0.031

9.635 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.011

6.245 ± 0.026

4.437 ± 0.026

4.047 ± 0.027

4.308 ± 0.019

8.633 ± 0.045

6.234 ± 0.029

5.479 ± 0.024

1.027 ± 0.009

3.306 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski