Spiribacter curvatus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Spiribacter

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1861 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5T2F3|U5T2F3_9GAMM Uncharacterized protein OS=Spiribacter curvatus OX=1335757 GN=SPICUR_02910 PE=4 SV=1
MM1 pKa = 7.06MKK3 pKa = 9.02KK4 pKa = 6.52TTSALAIAALLGTGAATAATFQINDD29 pKa = 3.57TTTVGVGGAFYY40 pKa = 10.4PFYY43 pKa = 11.3GDD45 pKa = 3.87VNDD48 pKa = 4.73ANGDD52 pKa = 3.7SSSFNGDD59 pKa = 2.76ASRR62 pKa = 11.84LIFTAEE68 pKa = 3.71KK69 pKa = 10.32AAVNGMTASLYY80 pKa = 9.75YY81 pKa = 9.62QLRR84 pKa = 11.84PQDD87 pKa = 3.83GLGDD91 pKa = 4.01GADD94 pKa = 3.98GSDD97 pKa = 3.92DD98 pKa = 3.79NNGVSTHH105 pKa = 5.37VAHH108 pKa = 6.67ATLSGDD114 pKa = 3.8FGSVTYY120 pKa = 10.42GKK122 pKa = 10.14NDD124 pKa = 3.05NLMYY128 pKa = 10.4RR129 pKa = 11.84YY130 pKa = 9.61IDD132 pKa = 3.41VLRR135 pKa = 11.84DD136 pKa = 3.6YY137 pKa = 11.62NDD139 pKa = 3.42TLVFTFSDD147 pKa = 4.68AIQRR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 11.84VATYY157 pKa = 9.75ASPDD161 pKa = 3.24FGGFGFEE168 pKa = 4.52VEE170 pKa = 4.9AEE172 pKa = 4.16LEE174 pKa = 4.22ADD176 pKa = 3.78DD177 pKa = 5.29SKK179 pKa = 10.41TPVSDD184 pKa = 4.02GSSSSFNAAAYY195 pKa = 10.4ADD197 pKa = 4.41LDD199 pKa = 3.91PVRR202 pKa = 11.84VHH204 pKa = 6.18ATYY207 pKa = 9.1TAGDD211 pKa = 3.96LQDD214 pKa = 4.37ADD216 pKa = 3.72SDD218 pKa = 4.19GAYY221 pKa = 10.49GLAAVTSINVVDD233 pKa = 5.24LSAVYY238 pKa = 10.01TNSQINADD246 pKa = 3.93DD247 pKa = 3.67QVITGLYY254 pKa = 10.3ASTDD258 pKa = 3.48YY259 pKa = 11.67GFGSLHH265 pKa = 6.88LGAQEE270 pKa = 3.84VDD272 pKa = 2.94VDD274 pKa = 3.81NAEE277 pKa = 4.1SRR279 pKa = 11.84TEE281 pKa = 3.85VIGRR285 pKa = 11.84VTYY288 pKa = 10.52DD289 pKa = 2.95IADD292 pKa = 3.54SVYY295 pKa = 10.64TSLEE299 pKa = 4.16YY300 pKa = 10.57ASKK303 pKa = 11.0DD304 pKa = 3.33ADD306 pKa = 3.54NDD308 pKa = 3.87AGDD311 pKa = 4.08GFGVALWYY319 pKa = 10.73GFF321 pKa = 3.68

Molecular weight: 33.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5T906|U5T906_9GAMM Bac_transf domain-containing protein OS=Spiribacter curvatus OX=1335757 GN=SPICUR_09155 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.5RR12 pKa = 11.84KK13 pKa = 9.44RR14 pKa = 11.84NHH16 pKa = 5.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.32GRR39 pKa = 11.84ARR41 pKa = 11.84LTPP44 pKa = 3.93

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1861

0

1861

600998

31

1839

322.9

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.065 ± 0.068

0.839 ± 0.014

6.425 ± 0.046

6.074 ± 0.054

3.215 ± 0.033

8.648 ± 0.051

2.309 ± 0.024

5.043 ± 0.041

1.824 ± 0.035

10.604 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.025

2.379 ± 0.027

5.04 ± 0.04

3.608 ± 0.029

8.17 ± 0.065

5.11 ± 0.033

5.226 ± 0.036

7.404 ± 0.053

1.345 ± 0.023

2.254 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski