Pseudohaliea rubra DSM 19751

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Pseudohaliea; Pseudohaliea rubra

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095VN58|A0A095VN58_9GAMM Dipeptide transport ATP-binding protein DppD OS=Pseudohaliea rubra DSM 19751 OX=1265313 GN=HRUBRA_02556 PE=4 SV=1
MM1 pKa = 6.75SHH3 pKa = 5.89WVATLGRR10 pKa = 11.84RR11 pKa = 11.84LAALGSMLVLLTACGGGGGGGGFLSDD37 pKa = 5.35DD38 pKa = 3.64GTGNNPLTITTGTLPDD54 pKa = 3.73VASTPYY60 pKa = 9.64STVLEE65 pKa = 4.47ASGGTPPYY73 pKa = 10.1SWEE76 pKa = 4.7LIDD79 pKa = 5.98DD80 pKa = 4.56GGTGFTINSEE90 pKa = 4.37GIFRR94 pKa = 11.84GDD96 pKa = 3.61ALPPAGTYY104 pKa = 10.46GVTISVSDD112 pKa = 3.6ANGRR116 pKa = 11.84DD117 pKa = 3.41TRR119 pKa = 11.84QSFTLTVTTEE129 pKa = 3.87NALSIATTALPQAIEE144 pKa = 4.11GLDD147 pKa = 3.48YY148 pKa = 10.58TALLDD153 pKa = 3.87ASGGAEE159 pKa = 4.02PYY161 pKa = 9.73RR162 pKa = 11.84WSVVNDD168 pKa = 3.48GGTGFGINADD178 pKa = 3.63GVLTGTAPDD187 pKa = 3.56SGDD190 pKa = 3.3YY191 pKa = 11.17GITLEE196 pKa = 4.44VTDD199 pKa = 4.61EE200 pKa = 4.09ADD202 pKa = 3.36SRR204 pKa = 11.84DD205 pKa = 3.76RR206 pKa = 11.84QSFILTVTGDD216 pKa = 3.6TPQPLTIATEE226 pKa = 4.12TLPTAEE232 pKa = 4.46EE233 pKa = 4.47GQTYY237 pKa = 10.47SAILQAAGGQGDD249 pKa = 4.42YY250 pKa = 11.28LWTLVDD256 pKa = 4.11AGGTGLDD263 pKa = 3.8LRR265 pKa = 11.84DD266 pKa = 4.47DD267 pKa = 4.3GVLSGTVPAEE277 pKa = 3.65GSYY280 pKa = 10.98GITVAVADD288 pKa = 4.03NVRR291 pKa = 11.84EE292 pKa = 4.06VSKK295 pKa = 11.21SLVLTVSAEE304 pKa = 4.01GDD306 pKa = 3.78PLTLGTTSLPGATVGEE322 pKa = 4.62RR323 pKa = 11.84YY324 pKa = 9.64AAVLEE329 pKa = 4.29ATGGSRR335 pKa = 11.84DD336 pKa = 3.8YY337 pKa = 11.26SWQLVSSGDD346 pKa = 3.57SGLSLSSAGVLSGTPSAVGTYY367 pKa = 10.18GIVFRR372 pKa = 11.84VSDD375 pKa = 3.99GQDD378 pKa = 3.0TVQGALTLVVDD389 pKa = 4.01TFEE392 pKa = 4.64PFVPLAVVTEE402 pKa = 4.14TLPAANGQIYY412 pKa = 10.15AATLEE417 pKa = 4.58AEE419 pKa = 4.87GGTPPYY425 pKa = 10.9SWTLVNDD432 pKa = 4.33GGSGLSLSTSGSLSGTSPAVGTYY455 pKa = 10.44GLTVSVADD463 pKa = 3.81SAGGSDD469 pKa = 3.42TAALTLEE476 pKa = 4.34VDD478 pKa = 3.85GSDD481 pKa = 3.05ATTLEE486 pKa = 4.73IISTDD491 pKa = 3.72LGTATQGEE499 pKa = 5.15TYY501 pKa = 10.9SFILRR506 pKa = 11.84ASGGPTGDD514 pKa = 3.77YY515 pKa = 8.37TWSKK519 pKa = 10.73VEE521 pKa = 4.23VVPSLPTIEE530 pKa = 4.63IGSDD534 pKa = 3.12SFEE537 pKa = 4.34DD538 pKa = 4.13ARR540 pKa = 11.84LTLDD544 pKa = 3.98PDD546 pKa = 3.85TGVLTWFSGDD556 pKa = 3.13IEE558 pKa = 4.7NGVLTGEE565 pKa = 4.39CTTDD569 pKa = 2.94YY570 pKa = 10.9SYY572 pKa = 10.82TVQVTDD578 pKa = 4.36AGPPASSDD586 pKa = 3.36VRR588 pKa = 11.84TLPLTAALDD597 pKa = 4.29PNDD600 pKa = 5.12PDD602 pKa = 3.94CQQ604 pKa = 3.59

Molecular weight: 61.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095VSJ5|A0A095VSJ5_9GAMM Permease of the drug/metabolite transporter (DMT) superfamily OS=Pseudohaliea rubra DSM 19751 OX=1265313 GN=HRUBRA_01389 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.35NGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2860

0

2860

961170

37

3013

336.1

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.862 ± 0.062

0.978 ± 0.016

6.007 ± 0.048

6.336 ± 0.039

3.554 ± 0.03

8.794 ± 0.043

2.078 ± 0.024

4.102 ± 0.032

2.189 ± 0.028

11.471 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.024 ± 0.02

2.43 ± 0.026

5.155 ± 0.027

3.146 ± 0.026

7.679 ± 0.054

5.037 ± 0.032

4.942 ± 0.045

7.333 ± 0.038

1.356 ± 0.019

2.528 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski