Vibrio sp. 10N.286.49.C2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; unclassified Vibrio

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4338 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N7D587|A0A2N7D587_9VIBR Formate transporter OS=Vibrio sp. 10N.286.49.C2 OX=1880856 GN=BCU70_16395 PE=4 SV=1
MM1 pKa = 7.67HH2 pKa = 6.55STKK5 pKa = 9.77LTALAGIIAFTLTGCGGEE23 pKa = 4.46SGSDD27 pKa = 3.39SPTSTTPFTLSVTDD41 pKa = 4.81APVDD45 pKa = 3.63DD46 pKa = 4.74AKK48 pKa = 11.17EE49 pKa = 3.93VVITFGKK56 pKa = 10.24VALLPQDD63 pKa = 3.62GGSPLIYY70 pKa = 10.15DD71 pKa = 4.23VYY73 pKa = 10.81LTDD76 pKa = 5.52DD77 pKa = 3.69EE78 pKa = 5.05GNPVDD83 pKa = 4.66EE84 pKa = 5.1SGEE87 pKa = 4.01LLPDD91 pKa = 3.94GADD94 pKa = 3.68TIPLSVNLLDD104 pKa = 4.23YY105 pKa = 10.84QGSASLPLIQDD116 pKa = 3.36QVVLTGRR123 pKa = 11.84YY124 pKa = 8.39KK125 pKa = 11.0LCVFANDD132 pKa = 4.09GDD134 pKa = 4.58HH135 pKa = 6.43PTHH138 pKa = 6.81RR139 pKa = 11.84SYY141 pKa = 11.84VVVDD145 pKa = 3.6EE146 pKa = 5.81DD147 pKa = 3.96EE148 pKa = 4.44TPEE151 pKa = 3.98NHH153 pKa = 7.2PGLSVKK159 pKa = 10.62GDD161 pKa = 3.66GACPQGVGKK170 pKa = 9.9EE171 pKa = 4.08DD172 pKa = 3.38KK173 pKa = 11.28AGVLYY178 pKa = 10.35FNNTFNVNADD188 pKa = 3.61NNDD191 pKa = 3.29FAVEE195 pKa = 4.04FDD197 pKa = 3.84LRR199 pKa = 11.84RR200 pKa = 11.84GLKK203 pKa = 9.84DD204 pKa = 3.21ASSAPDD210 pKa = 3.27YY211 pKa = 9.89TIQRR215 pKa = 11.84TSVSLINTVTTGNIEE230 pKa = 4.12GTIDD234 pKa = 3.18TATFSTCHH242 pKa = 5.61PQEE245 pKa = 5.01DD246 pKa = 4.25LTSVQSVYY254 pKa = 10.83LYY256 pKa = 10.67NSNVAQEE263 pKa = 4.03NMAPIGGSDD272 pKa = 3.43EE273 pKa = 4.75SKK275 pKa = 10.34PLTSASVNWNEE286 pKa = 3.93SLAAYY291 pKa = 7.25TFSFGFMEE299 pKa = 4.82PGSYY303 pKa = 10.66NLGYY307 pKa = 8.78TCTAQFDD314 pKa = 4.33GGEE317 pKa = 4.2EE318 pKa = 3.94NHH320 pKa = 7.09DD321 pKa = 3.53PVAAGFSIYY330 pKa = 10.43DD331 pKa = 3.59SQSDD335 pKa = 3.73VTVAVGKK342 pKa = 8.19DD343 pKa = 3.31TKK345 pKa = 11.27VLFF348 pKa = 4.4

Molecular weight: 36.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N7D483|A0A2N7D483_9VIBR tRNA modification GTPase MnmE OS=Vibrio sp. 10N.286.49.C2 OX=1880856 GN=mnmE PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.49RR13 pKa = 11.84KK14 pKa = 7.97RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.46NGRR29 pKa = 11.84KK30 pKa = 7.78TINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84LSKK45 pKa = 10.83

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4338

0

4338

1422617

35

4079

327.9

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.502 ± 0.036

1.004 ± 0.011

5.655 ± 0.043

6.067 ± 0.033

4.16 ± 0.027

6.941 ± 0.039

2.301 ± 0.018

6.471 ± 0.031

5.106 ± 0.03

10.152 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.759 ± 0.022

4.273 ± 0.026

3.867 ± 0.025

4.387 ± 0.028

4.401 ± 0.029

6.787 ± 0.03

5.629 ± 0.032

7.221 ± 0.031

1.278 ± 0.018

3.039 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski