Cybaeus spider associated circular virus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus cybusi1

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BP79|A0A346BP79_9VIRU Putative capsid protein OS=Cybaeus spider associated circular virus 2 OX=2293278 PE=4 SV=1
MM1 pKa = 8.01PFRR4 pKa = 11.84FQAKK8 pKa = 9.96YY9 pKa = 11.08GLLTYY14 pKa = 9.02AQCGDD19 pKa = 3.76LDD21 pKa = 3.69AFAIVDD27 pKa = 3.78HH28 pKa = 6.67LSEE31 pKa = 5.05LGAEE35 pKa = 4.44CIVGEE40 pKa = 4.46EE41 pKa = 3.98LHH43 pKa = 7.03ADD45 pKa = 4.18GGRR48 pKa = 11.84HH49 pKa = 4.36LHH51 pKa = 6.16AFFMFQHH58 pKa = 7.09KK59 pKa = 9.71FRR61 pKa = 11.84TSNARR66 pKa = 11.84VFDD69 pKa = 3.52VGGNHH74 pKa = 7.03ANISPGYY81 pKa = 9.2SNPSDD86 pKa = 3.89GYY88 pKa = 11.29DD89 pKa = 3.44YY90 pKa = 10.99AIKK93 pKa = 10.53DD94 pKa = 3.79GNILAGGLEE103 pKa = 4.2RR104 pKa = 11.84PGNEE108 pKa = 4.36SPKK111 pKa = 10.71DD112 pKa = 3.44DD113 pKa = 4.64SKK115 pKa = 11.12WHH117 pKa = 6.66TITAAEE123 pKa = 4.32TEE125 pKa = 4.51SEE127 pKa = 4.36FWALVAQLDD136 pKa = 3.99PRR138 pKa = 11.84SMVVSYY144 pKa = 9.43PALRR148 pKa = 11.84AYY150 pKa = 10.2AGNRR154 pKa = 11.84YY155 pKa = 9.13RR156 pKa = 11.84VDD158 pKa = 4.62RR159 pKa = 11.84IPYY162 pKa = 9.32RR163 pKa = 11.84NPDD166 pKa = 3.31GLRR169 pKa = 11.84YY170 pKa = 8.73CTDD173 pKa = 3.85EE174 pKa = 4.22YY175 pKa = 10.83PHH177 pKa = 8.16LDD179 pKa = 3.07EE180 pKa = 4.49WVEE183 pKa = 3.9GSLRR187 pKa = 11.84NNRR190 pKa = 11.84EE191 pKa = 3.47NRR193 pKa = 11.84PRR195 pKa = 11.84SLVLYY200 pKa = 10.49GGTRR204 pKa = 11.84LGKK207 pKa = 7.42TLWSRR212 pKa = 11.84SLGNHH217 pKa = 7.02AYY219 pKa = 10.42FGGLFAMDD227 pKa = 4.37EE228 pKa = 4.81PIDD231 pKa = 4.2DD232 pKa = 2.98VDD234 pKa = 3.84YY235 pKa = 11.56AVFDD239 pKa = 4.27DD240 pKa = 4.21MQGGLDD246 pKa = 3.81FFHH249 pKa = 7.45SYY251 pKa = 10.7KK252 pKa = 10.46FWLGAQKK259 pKa = 10.36QFYY262 pKa = 8.76VTDD265 pKa = 3.67KK266 pKa = 11.21YY267 pKa = 10.97KK268 pKa = 10.96GKK270 pKa = 10.72KK271 pKa = 8.43LVSWGKK277 pKa = 8.75PCIYY281 pKa = 10.26LSNNDD286 pKa = 3.16PRR288 pKa = 11.84GDD290 pKa = 3.57KK291 pKa = 10.95SCDD294 pKa = 3.41IEE296 pKa = 4.22WLEE299 pKa = 4.29GNCTIVYY306 pKa = 9.08IDD308 pKa = 3.88RR309 pKa = 11.84PIFRR313 pKa = 11.84ANTEE317 pKa = 3.84

Molecular weight: 36.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BP79|A0A346BP79_9VIRU Putative capsid protein OS=Cybaeus spider associated circular virus 2 OX=2293278 PE=4 SV=1
MM1 pKa = 7.03VFRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84STLRR10 pKa = 11.84SRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84PARR17 pKa = 11.84RR18 pKa = 11.84TRR20 pKa = 11.84RR21 pKa = 11.84SNPRR25 pKa = 11.84RR26 pKa = 11.84SYY28 pKa = 10.44AKK30 pKa = 9.36KK31 pKa = 9.88RR32 pKa = 11.84SFKK35 pKa = 10.66RR36 pKa = 11.84RR37 pKa = 11.84TTTRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84FPSTKK48 pKa = 9.6MILNKK53 pKa = 10.08TSTKK57 pKa = 10.29KK58 pKa = 9.55RR59 pKa = 11.84DD60 pKa = 3.01KK61 pKa = 10.13MLPHH65 pKa = 6.95GSANTDD71 pKa = 2.75ADD73 pKa = 3.26IAGGVTISSGDD84 pKa = 3.53GYY86 pKa = 9.9FTSGWIATARR96 pKa = 11.84DD97 pKa = 3.4KK98 pKa = 11.26FKK100 pKa = 11.35NDD102 pKa = 2.69GSLGTKK108 pKa = 10.34AEE110 pKa = 4.17MAVRR114 pKa = 11.84TAQTCFMVGLRR125 pKa = 11.84EE126 pKa = 3.96NVYY129 pKa = 10.84LRR131 pKa = 11.84TDD133 pKa = 5.09DD134 pKa = 3.89GASWQWRR141 pKa = 11.84RR142 pKa = 11.84ICFTFKK148 pKa = 10.75GSGIINNDD156 pKa = 3.36SQDD159 pKa = 3.52SSDD162 pKa = 3.71SGWFRR167 pKa = 11.84EE168 pKa = 4.1TSNGFVRR175 pKa = 11.84YY176 pKa = 9.16IHH178 pKa = 6.11QVPLGNNLLQVLFKK192 pKa = 11.04GALNVDD198 pKa = 2.98WTDD201 pKa = 3.13QFTAPVDD208 pKa = 3.68TTRR211 pKa = 11.84VSLKK215 pKa = 10.04YY216 pKa = 10.31DD217 pKa = 2.97KK218 pKa = 10.05QTIIRR223 pKa = 11.84SGNEE227 pKa = 3.45EE228 pKa = 4.06GVGRR232 pKa = 11.84MHH234 pKa = 6.92KK235 pKa = 9.39RR236 pKa = 11.84WHH238 pKa = 5.58GMYY241 pKa = 10.29HH242 pKa = 5.9NLVYY246 pKa = 10.89DD247 pKa = 3.7EE248 pKa = 5.82DD249 pKa = 4.79EE250 pKa = 5.48IGDD253 pKa = 4.1SMTDD257 pKa = 3.04NNLSVEE263 pKa = 4.56AKK265 pKa = 10.12PGMGDD270 pKa = 3.56YY271 pKa = 10.97YY272 pKa = 10.87VIDD275 pKa = 3.66MFRR278 pKa = 11.84CQSSGTDD285 pKa = 3.05HH286 pKa = 7.1EE287 pKa = 5.96LFFQPEE293 pKa = 4.22STLYY297 pKa = 9.05WHH299 pKa = 7.11EE300 pKa = 4.13KK301 pKa = 9.32

Molecular weight: 34.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

618

301

317

309.0

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.987 ± 0.974

1.456 ± 0.335

7.929 ± 0.452

4.531 ± 0.639

5.178 ± 0.142

8.738 ± 0.557

2.751 ± 0.31

4.045 ± 0.043

5.178 ± 0.585

6.796 ± 1.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.653

5.016 ± 0.024

3.722 ± 0.776

2.589 ± 0.292

8.738 ± 1.623

7.12 ± 1.107

5.987 ± 1.933

4.854 ± 0.094

2.265 ± 0.044

4.693 ± 0.999
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski