Vulpes vulpes (Red fox)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q7R749|A0A3Q7R749_VULVU serine/threonine-protein kinase Chk2 isoform X1 OS=Vulpes vulpes OX=9627 GN=CHEK2 PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 6.69ADD4 pKa = 4.76PEE6 pKa = 4.56TQSSCEE12 pKa = 3.95PEE14 pKa = 3.74AARR17 pKa = 11.84SVVEE21 pKa = 3.92AEE23 pKa = 3.9EE24 pKa = 5.6DD25 pKa = 3.71EE26 pKa = 4.8GCLEE30 pKa = 4.2SSHH33 pKa = 5.18TRR35 pKa = 11.84KK36 pKa = 10.0RR37 pKa = 11.84SCIPSRR43 pKa = 11.84GSWHH47 pKa = 6.91LLLPQALLQLLTDD60 pKa = 4.87PAPAPDD66 pKa = 4.35PALAPDD72 pKa = 5.17PDD74 pKa = 4.65PAPDD78 pKa = 4.2PAPAPDD84 pKa = 4.43SVPAPDD90 pKa = 4.41SAPAPDD96 pKa = 4.18PAAALASATTSVPCPHH112 pKa = 6.58TTPPPAPAPAPAPAAASDD130 pKa = 3.79PAPARR135 pKa = 11.84EE136 pKa = 4.09TAPDD140 pKa = 3.63PAAAPVPAPTPATASCPEE158 pKa = 4.08STPASNTDD166 pKa = 3.85PVPSPAPDD174 pKa = 3.98PSPDD178 pKa = 4.08CDD180 pKa = 4.22HH181 pKa = 7.52DD182 pKa = 4.16PAPASAPDD190 pKa = 3.87PALTSAPDD198 pKa = 3.92DD199 pKa = 4.58DD200 pKa = 4.84LASAAVPAPALNTEE214 pKa = 4.96FGPDD218 pKa = 3.72PVPAPDD224 pKa = 4.9LEE226 pKa = 5.63NDD228 pKa = 4.46LSPAPAPAAAPAGAPAPDD246 pKa = 3.46TDD248 pKa = 4.6FPFDD252 pKa = 3.79SAPDD256 pKa = 3.75TEE258 pKa = 4.95LAPACTPDD266 pKa = 3.68PNLAHH271 pKa = 6.52VPAPVPVPDD280 pKa = 3.82TASANTPAPDD290 pKa = 3.55NDD292 pKa = 4.26LAPSPSPDD300 pKa = 3.65PAPVCDD306 pKa = 4.43TDD308 pKa = 4.41CSSASAPDD316 pKa = 3.84NEE318 pKa = 4.7PASTPALDD326 pKa = 4.61LAPHH330 pKa = 6.87TDD332 pKa = 3.86PAPTPAPAPTCAPGTDD348 pKa = 4.06TDD350 pKa = 4.6PVPAPDD356 pKa = 4.17NDD358 pKa = 4.36LAPGPAPDD366 pKa = 3.9TAPGPVIAPAPGLALVPTPGLAPPPDD392 pKa = 3.83NDD394 pKa = 3.43FASAPGPDD402 pKa = 4.75MYY404 pKa = 10.65PAPAPGLDD412 pKa = 4.07RR413 pKa = 11.84DD414 pKa = 4.24PTPAPAPPPVPAGLPEE430 pKa = 4.13THH432 pKa = 7.16LEE434 pKa = 3.99PTALRR439 pKa = 11.84GDD441 pKa = 4.61LLVQSPFSPSPDD453 pKa = 3.77PDD455 pKa = 4.09PLPTCHH461 pKa = 7.43PLPLWSFFLVSVHH474 pKa = 6.21CLSIFTHH481 pKa = 6.6SDD483 pKa = 3.12LFQKK487 pKa = 9.22VVPYY491 pKa = 10.71SCLGYY496 pKa = 9.61MRR498 pKa = 11.84SQQVKK503 pKa = 9.78KK504 pKa = 10.7SNEE507 pKa = 3.87HH508 pKa = 6.32LPPTVHH514 pKa = 7.67AIITT518 pKa = 3.75

Molecular weight: 51.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q7UJM5|A0A3Q7UJM5_VULVU GTPase IMAP family member GIMD1 OS=Vulpes vulpes OX=9627 GN=GIMD1 PE=3 SV=1
PP1 pKa = 7.05HH2 pKa = 7.7RR3 pKa = 11.84APGAWSLLLPLPMSMSSHH21 pKa = 4.44KK22 pKa = 8.94TFRR25 pKa = 11.84IKK27 pKa = 10.64RR28 pKa = 11.84FLAKK32 pKa = 9.71KK33 pKa = 9.58QKK35 pKa = 8.69QNRR38 pKa = 11.84PIPQWIQMKK47 pKa = 8.88TGNKK51 pKa = 8.6IRR53 pKa = 11.84YY54 pKa = 7.09NSKK57 pKa = 8.3RR58 pKa = 11.84RR59 pKa = 11.84HH60 pKa = 3.95WRR62 pKa = 11.84RR63 pKa = 11.84TKK65 pKa = 10.83LGLL68 pKa = 3.67

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19316

12868

32184

21246518

15

34362

660.2

73.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.955 ± 0.015

2.115 ± 0.01

4.857 ± 0.009

7.257 ± 0.019

3.497 ± 0.008

6.482 ± 0.019

2.572 ± 0.007

4.317 ± 0.013

5.8 ± 0.019

9.806 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.006

3.583 ± 0.009

6.47 ± 0.025

4.875 ± 0.014

5.736 ± 0.013

8.516 ± 0.02

5.316 ± 0.013

5.952 ± 0.014

1.139 ± 0.004

2.567 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski