Synechococcus sp. CB0101

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7ZWJ8|A0A4P7ZWJ8_9SYNE CPBP family intramembrane metalloprotease OS=Synechococcus sp. CB0101 OX=232348 GN=CB0101_10125 PE=4 SV=1
MM1 pKa = 7.36SLAPINLSEE10 pKa = 4.5AAQDD14 pKa = 3.82MASGDD19 pKa = 4.04YY20 pKa = 11.18GDD22 pKa = 5.97LDD24 pKa = 3.54AQEE27 pKa = 4.68ILLEE31 pKa = 4.1FAPFLQPPYY40 pKa = 10.01VVAGVGLAMGVLCGLTFARR59 pKa = 11.84LVQDD63 pKa = 5.09KK64 pKa = 11.26LNGWKK69 pKa = 9.36QDD71 pKa = 3.53RR72 pKa = 11.84LALLPLGCFEE82 pKa = 4.99TNLSYY87 pKa = 10.7TGIVLGITLFIGGSLQVFSFAAGAAYY113 pKa = 9.98LVALLLSLATAGALWVQLEE132 pKa = 3.94RR133 pKa = 11.84LMLQVEE139 pKa = 4.58NGTFSAVDD147 pKa = 4.08FDD149 pKa = 5.47NFDD152 pKa = 3.17QFFF155 pKa = 3.61

Molecular weight: 16.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7ZVG5|A0A4P7ZVG5_9SYNE Uncharacterized protein OS=Synechococcus sp. CB0101 OX=232348 GN=CB0101_11625 PE=4 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.03RR4 pKa = 11.84TLEE7 pKa = 3.92GTSRR11 pKa = 11.84KK12 pKa = 9.24RR13 pKa = 11.84KK14 pKa = 7.95RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2840

0

2840

801788

30

1527

282.3

30.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.746 ± 0.063

1.205 ± 0.016

4.792 ± 0.033

5.819 ± 0.047

2.943 ± 0.032

8.478 ± 0.046

2.083 ± 0.027

3.863 ± 0.035

2.372 ± 0.038

13.015 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.838 ± 0.019

2.442 ± 0.034

5.843 ± 0.034

5.15 ± 0.043

7.308 ± 0.047

6.001 ± 0.036

4.433 ± 0.037

6.96 ± 0.044

1.841 ± 0.03

1.867 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski