Weeksella virosa (strain ATCC 43766 / DSM 16922 / JCM 21250 / NBRC 16016 / NCTC 11634 / CL345/78)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Weeksella; Weeksella virosa

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2045 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0P2G6|F0P2G6_WEEVC Uncharacterized protein OS=Weeksella virosa (strain ATCC 43766 / DSM 16922 / JCM 21250 / NBRC 16016 / NCTC 11634 / CL345/78) OX=865938 GN=Weevi_0050 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.38NKK4 pKa = 9.88TNFCEE9 pKa = 4.17EE10 pKa = 5.15DD11 pKa = 3.08ISEE14 pKa = 4.67LKK16 pKa = 10.83DD17 pKa = 3.27GLTPFPEE24 pKa = 4.21EE25 pKa = 3.86VFVAEE30 pKa = 4.23QSWLNDD36 pKa = 3.22CFLPLISVDD45 pKa = 4.0LGILRR50 pKa = 11.84TDD52 pKa = 3.73LAGTVVHH59 pKa = 6.66FLNPVEE65 pKa = 4.49PADD68 pKa = 3.84GLLGEE73 pKa = 4.45EE74 pKa = 4.16TEE76 pKa = 4.18EE77 pKa = 4.07FHH79 pKa = 8.1NEE81 pKa = 3.24FCAEE85 pKa = 3.38NWIAFKK91 pKa = 10.08LTTDD95 pKa = 2.97NKK97 pKa = 10.83YY98 pKa = 11.26NFLADD103 pKa = 3.36KK104 pKa = 10.68DD105 pKa = 4.3YY106 pKa = 11.07FLSLSEE112 pKa = 4.37CDD114 pKa = 3.24EE115 pKa = 4.77DD116 pKa = 4.51LAEE119 pKa = 5.49HH120 pKa = 6.27IQTMRR125 pKa = 11.84DD126 pKa = 2.88TFQTVKK132 pKa = 10.47SKK134 pKa = 11.17YY135 pKa = 9.22KK136 pKa = 10.68EE137 pKa = 3.92KK138 pKa = 10.77GQLLSWQDD146 pKa = 3.4YY147 pKa = 9.96PDD149 pKa = 3.78ALNFIDD155 pKa = 5.38RR156 pKa = 11.84LDD158 pKa = 3.92GEE160 pKa = 4.47ILGGNWVDD168 pKa = 3.75TVDD171 pKa = 3.4IPSAFEE177 pKa = 4.13MNFEE181 pKa = 4.36TPPEE185 pKa = 4.48DD186 pKa = 3.71SDD188 pKa = 4.26SDD190 pKa = 4.37GISISYY196 pKa = 8.91QGKK199 pKa = 7.86EE200 pKa = 3.62LMYY203 pKa = 9.31VGEE206 pKa = 4.27VAGYY210 pKa = 9.01NYY212 pKa = 10.32CSEE215 pKa = 4.21GADD218 pKa = 3.26AIMIFYY224 pKa = 10.14EE225 pKa = 3.99PEE227 pKa = 3.44NRR229 pKa = 11.84IVLFTYY235 pKa = 10.08DD236 pKa = 2.92WSS238 pKa = 3.73

Molecular weight: 27.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0NXY9|F0NXY9_WEEVC Putative mannosyltransferase OS=Weeksella virosa (strain ATCC 43766 / DSM 16922 / JCM 21250 / NBRC 16016 / NCTC 11634 / CL345/78) OX=865938 GN=Weevi_1354 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.89HH16 pKa = 3.92GFRR19 pKa = 11.84EE20 pKa = 4.1RR21 pKa = 11.84MSTKK25 pKa = 9.7NGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.85KK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.68ALTISEE46 pKa = 4.15VRR48 pKa = 11.84AKK50 pKa = 10.5RR51 pKa = 3.41

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2045

0

2045

668002

31

2351

326.7

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.245 ± 0.05

0.695 ± 0.015

5.397 ± 0.041

6.908 ± 0.056

5.221 ± 0.044

6.088 ± 0.051

1.895 ± 0.023

8.056 ± 0.053

7.764 ± 0.05

9.394 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.022

6.001 ± 0.054

3.411 ± 0.029

4.034 ± 0.035

3.731 ± 0.035

5.977 ± 0.04

5.514 ± 0.034

6.151 ± 0.047

1.012 ± 0.018

4.223 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski