Streptococcus phage TP-778L

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6E9F5|U6E9F5_9CAUD Uncharacterized protein OS=Streptococcus phage TP-778L OX=1385385 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVSWFVIHH14 pKa = 7.56DD15 pKa = 3.9GKK17 pKa = 9.65LTYY20 pKa = 11.14SMYY23 pKa = 10.84GSRR26 pKa = 11.84NGSDD30 pKa = 2.98GTADD34 pKa = 3.56CSGSMSQALKK44 pKa = 10.66DD45 pKa = 3.47AGIAIQGLPSTVTLGQQLAKK65 pKa = 10.58NGFYY69 pKa = 10.18RR70 pKa = 11.84VSINQDD76 pKa = 2.52WEE78 pKa = 4.16ALTGDD83 pKa = 3.79IVMMSWGADD92 pKa = 3.23MSQSGGAGGHH102 pKa = 5.75VGVMMDD108 pKa = 3.33ATYY111 pKa = 9.9FISCDD116 pKa = 3.38YY117 pKa = 9.4STQGAVGQAINTYY130 pKa = 8.91PWNDD134 pKa = 3.08YY135 pKa = 10.1YY136 pKa = 11.51AANKK140 pKa = 6.83PTYY143 pKa = 9.85IEE145 pKa = 3.7VWRR148 pKa = 11.84YY149 pKa = 10.07ADD151 pKa = 5.12SAPQTNNKK159 pKa = 9.87ANTAVVTQEE168 pKa = 3.59KK169 pKa = 10.29AYY171 pKa = 11.09YY172 pKa = 8.32EE173 pKa = 4.29ANEE176 pKa = 3.96VKK178 pKa = 10.25YY179 pKa = 11.21VNGIWQIKK187 pKa = 9.38CDD189 pKa = 4.05YY190 pKa = 7.4LTPIGFDD197 pKa = 2.86WLQNGIPVSMVNWVDD212 pKa = 3.89ANGNDD217 pKa = 3.63IPDD220 pKa = 4.4GADD223 pKa = 2.92QDD225 pKa = 4.58FKK227 pKa = 11.75AGMFFSFSGDD237 pKa = 2.98EE238 pKa = 4.17TNIVDD243 pKa = 3.97TGNGGYY249 pKa = 10.39YY250 pKa = 9.99GGYY253 pKa = 8.35YY254 pKa = 8.81WRR256 pKa = 11.84QFEE259 pKa = 4.32FGQFGPVWLSCWNKK273 pKa = 10.66DD274 pKa = 4.61DD275 pKa = 4.6LVNYY279 pKa = 9.18YY280 pKa = 10.29KK281 pKa = 10.97

Molecular weight: 31.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6E9A9|U6E9A9_9CAUD Uncharacterized protein OS=Streptococcus phage TP-778L OX=1385385 PE=4 SV=1
MM1 pKa = 7.41ASYY4 pKa = 10.61RR5 pKa = 11.84KK6 pKa = 9.62RR7 pKa = 11.84SNGWEE12 pKa = 3.78YY13 pKa = 10.71RR14 pKa = 11.84INYY17 pKa = 8.82YY18 pKa = 10.97DD19 pKa = 3.3STGKK23 pKa = 10.38RR24 pKa = 11.84KK25 pKa = 9.53PKK27 pKa = 9.3TKK29 pKa = 10.39GGFRR33 pKa = 11.84TKK35 pKa = 10.72SEE37 pKa = 4.42AIKK40 pKa = 10.46AAAEE44 pKa = 4.18MEE46 pKa = 5.02LKK48 pKa = 10.8LQDD51 pKa = 3.77NVNVDD56 pKa = 3.82EE57 pKa = 6.69DD58 pKa = 3.63ITLYY62 pKa = 10.83DD63 pKa = 4.96YY64 pKa = 10.5FKK66 pKa = 10.61QWCEE70 pKa = 3.93VYY72 pKa = 10.59KK73 pKa = 10.79KK74 pKa = 9.15PTVSKK79 pKa = 8.97ITYY82 pKa = 8.32KK83 pKa = 10.81AYY85 pKa = 9.82INSQRR90 pKa = 11.84KK91 pKa = 8.68IEE93 pKa = 4.5LFFGDD98 pKa = 4.86KK99 pKa = 9.88KK100 pKa = 10.85LKK102 pKa = 10.68SITATEE108 pKa = 4.07YY109 pKa = 10.51QRR111 pKa = 11.84VLNSYY116 pKa = 10.88AKK118 pKa = 8.91THH120 pKa = 5.99AQDD123 pKa = 3.07TVEE126 pKa = 4.03RR127 pKa = 11.84FNVHH131 pKa = 5.35VKK133 pKa = 9.37TKK135 pKa = 9.86IYY137 pKa = 10.33KK138 pKa = 9.36II139 pKa = 3.88

Molecular weight: 16.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12834

46

2020

246.8

27.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.511 ± 0.866

0.553 ± 0.099

6.218 ± 0.43

6.584 ± 0.57

4.005 ± 0.193

6.989 ± 0.509

1.239 ± 0.155

6.841 ± 0.409

7.87 ± 0.511

7.815 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.266

5.649 ± 0.324

2.961 ± 0.262

3.826 ± 0.208

4.223 ± 0.326

7.013 ± 0.501

6.607 ± 0.387

6.452 ± 0.236

1.325 ± 0.207

4.021 ± 0.437
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski