Propionispora sp. 2/2-37

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Propionispora; unclassified Propionispora

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K8JFJ5|A0A0K8JFJ5_9FIRM Protein-glutamate methylesterase/protein-glutamine glutaminase OS=Propionispora sp. 2/2-37 OX=1677858 GN=cheB PE=3 SV=1
MM1 pKa = 7.55LRR3 pKa = 11.84ITDD6 pKa = 3.84SMVTAQTLANINNNTKK22 pKa = 10.32RR23 pKa = 11.84LSEE26 pKa = 4.22ANDD29 pKa = 3.15RR30 pKa = 11.84VASGKK35 pKa = 10.69KK36 pKa = 9.03IDD38 pKa = 4.42EE39 pKa = 4.8ASDD42 pKa = 3.86DD43 pKa = 3.76PTVAARR49 pKa = 11.84AIKK52 pKa = 9.14YY53 pKa = 9.32RR54 pKa = 11.84NYY56 pKa = 9.14VAKK59 pKa = 10.4IEE61 pKa = 4.05QYY63 pKa = 10.76QDD65 pKa = 2.87NASAATSWQKK75 pKa = 9.38VTDD78 pKa = 4.12DD79 pKa = 5.5AMDD82 pKa = 4.79DD83 pKa = 3.92LDD85 pKa = 6.15DD86 pKa = 4.64VIQRR90 pKa = 11.84LQEE93 pKa = 3.78ITTQAASTATSSDD106 pKa = 2.95TDD108 pKa = 3.64MEE110 pKa = 4.36ALQQEE115 pKa = 4.75VTEE118 pKa = 4.45LQQQLVDD125 pKa = 3.94ILNTSYY131 pKa = 11.2AGRR134 pKa = 11.84YY135 pKa = 7.66VFAGYY140 pKa = 7.95DD141 pKa = 3.31TGEE144 pKa = 3.9APYY147 pKa = 11.2ALVQTYY153 pKa = 7.65TAADD157 pKa = 3.59VTTTDD162 pKa = 3.37DD163 pKa = 2.9TGYY166 pKa = 9.2TEE168 pKa = 5.45DD169 pKa = 4.36SIGGITGLTAGSSYY183 pKa = 10.56TLTVADD189 pKa = 3.65STAYY193 pKa = 10.27AGAYY197 pKa = 10.13SYY199 pKa = 10.44TLTDD203 pKa = 3.35ANGNIVASVDD213 pKa = 3.73NITDD217 pKa = 3.28TTTAVTLTGTDD228 pKa = 3.81DD229 pKa = 4.02EE230 pKa = 5.72SITLAGTTGVTTGDD244 pKa = 3.08MTFTVNNSSLGSTITYY260 pKa = 9.34KK261 pKa = 11.06GQVLSLCGVVDD272 pKa = 4.31SSVSDD277 pKa = 4.16DD278 pKa = 5.53DD279 pKa = 4.42IISYY283 pKa = 7.97YY284 pKa = 8.73TANASVEE291 pKa = 3.91YY292 pKa = 10.49DD293 pKa = 3.24AANSGTQAIKK303 pKa = 10.92YY304 pKa = 8.55NIGYY308 pKa = 9.79GIEE311 pKa = 3.72IAVNVEE317 pKa = 3.96GQDD320 pKa = 3.39ITGTGADD327 pKa = 4.15DD328 pKa = 3.65TNLFDD333 pKa = 4.46TVAKK337 pKa = 10.59LLLSLDD343 pKa = 3.59GDD345 pKa = 4.18TSYY348 pKa = 9.41KK349 pKa = 9.84TYY351 pKa = 10.82NSEE354 pKa = 3.88TGEE357 pKa = 4.18VEE359 pKa = 4.44TVSFTTDD366 pKa = 2.82EE367 pKa = 4.85LLTEE371 pKa = 4.43LQTNEE376 pKa = 4.25DD377 pKa = 3.63RR378 pKa = 11.84LLTTRR383 pKa = 11.84TDD385 pKa = 3.13LGARR389 pKa = 11.84MSYY392 pKa = 9.97TSTVTSRR399 pKa = 11.84LSNDD403 pKa = 2.5LTTYY407 pKa = 11.02KK408 pKa = 10.37EE409 pKa = 4.22FKK411 pKa = 10.35SDD413 pKa = 4.23NEE415 pKa = 4.2DD416 pKa = 2.91VDD418 pKa = 3.45IAEE421 pKa = 4.82AATEE425 pKa = 4.06QATAEE430 pKa = 4.16YY431 pKa = 10.2VYY433 pKa = 10.44QASLTVGASVISKK446 pKa = 8.65TLVDD450 pKa = 3.86YY451 pKa = 10.65LAA453 pKa = 5.2

Molecular weight: 48.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K8JAE7|A0A0K8JAE7_9FIRM Glucitol/sorbitol permease IIC component OS=Propionispora sp. 2/2-37 OX=1677858 GN=srlA PE=4 SV=1
MM1 pKa = 7.85WEE3 pKa = 4.25MLLLAAGLWGLQGVLGFFQMRR24 pKa = 11.84NFNGRR29 pKa = 11.84FKK31 pKa = 11.12ALRR34 pKa = 11.84QRR36 pKa = 11.84GRR38 pKa = 11.84VVAGRR43 pKa = 11.84SKK45 pKa = 9.99GRR47 pKa = 11.84MVAGVVVLFCIDD59 pKa = 3.32ANGTILTGEE68 pKa = 4.15RR69 pKa = 11.84MSGVSSFARR78 pKa = 11.84FHH80 pKa = 6.8PFPALQGNQLNKK92 pKa = 9.43IDD94 pKa = 4.1EE95 pKa = 4.86TICEE99 pKa = 4.44YY100 pKa = 10.61IRR102 pKa = 11.84LDD104 pKa = 3.57RR105 pKa = 11.84QTTRR109 pKa = 11.84AVLNAVDD116 pKa = 4.01NYY118 pKa = 11.06RR119 pKa = 11.84AFIQANTTMEE129 pKa = 4.48VIPAKK134 pKa = 10.06TGALLPP140 pKa = 4.51

Molecular weight: 15.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3949

0

3949

1189072

29

2502

301.1

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.888 ± 0.045

1.172 ± 0.016

4.904 ± 0.029

6.408 ± 0.037

3.931 ± 0.028

7.491 ± 0.044

1.952 ± 0.018

7.394 ± 0.035

5.86 ± 0.032

9.877 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.02

4.026 ± 0.03

3.83 ± 0.026

3.916 ± 0.029

4.829 ± 0.031

5.615 ± 0.032

5.342 ± 0.033

7.441 ± 0.035

0.975 ± 0.014

3.379 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski