Streptococcus pneumoniae 2070335

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus pneumoniae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2823 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J1P0Z7|J1P0Z7_STREE Polyamine aminopropyltransferase OS=Streptococcus pneumoniae 2070335 OX=914141 GN=speE PE=3 SV=1
MM1 pKa = 7.29NLSFNIKK8 pKa = 10.12IIDD11 pKa = 3.75EE12 pKa = 4.13NSFAEE17 pKa = 4.5LSDD20 pKa = 4.48MITGDD25 pKa = 3.97YY26 pKa = 9.64ITFLSSNDD34 pKa = 3.7SLFDD38 pKa = 3.21WTFEE42 pKa = 4.16KK43 pKa = 10.01MYY45 pKa = 10.48HH46 pKa = 6.34AIKK49 pKa = 10.68LSDD52 pKa = 3.51SDD54 pKa = 3.99VVLGHH59 pKa = 6.65FADD62 pKa = 4.09LVDD65 pKa = 3.77GVFYY69 pKa = 8.85FYY71 pKa = 10.58PLGGIIGG78 pKa = 3.71

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J1NTB8|J1NTB8_STREE Histidine kinase domain protein OS=Streptococcus pneumoniae 2070335 OX=914141 GN=AMCSP13_002389 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.24QPSKK9 pKa = 9.82LRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.57HH16 pKa = 4.75GFRR19 pKa = 11.84NRR21 pKa = 11.84MSTKK25 pKa = 9.22NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 8.75VLAAA44 pKa = 4.31

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2823

0

2823

582959

37

1463

206.5

23.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.358 ± 0.063

0.619 ± 0.015

5.499 ± 0.044

7.202 ± 0.06

4.614 ± 0.043

6.59 ± 0.054

1.913 ± 0.024

7.191 ± 0.05

6.764 ± 0.049

10.262 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.611 ± 0.026

4.356 ± 0.041

3.319 ± 0.031

4.12 ± 0.037

4.099 ± 0.04

6.256 ± 0.053

5.49 ± 0.039

6.991 ± 0.044

0.965 ± 0.019

3.779 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski