Sphingomonas sp. Leaf20

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4FU37|A0A0Q4FU37_9SPHN Uncharacterized protein OS=Sphingomonas sp. Leaf20 OX=1735685 GN=ASE72_17950 PE=4 SV=1
MM1 pKa = 7.21TFEE4 pKa = 5.06ASGSTAGFNDD14 pKa = 3.33GGTYY18 pKa = 10.47NDD20 pKa = 4.46GGPVPLAASLDD31 pKa = 4.02PNAGGTSNDD40 pKa = 3.15KK41 pKa = 10.4TIWTLDD47 pKa = 3.4QINANFNRR55 pKa = 11.84SGYY58 pKa = 10.41DD59 pKa = 2.68WYY61 pKa = 9.94TNNYY65 pKa = 9.53GEE67 pKa = 5.3LNDD70 pKa = 4.33GVLNYY75 pKa = 9.97GFWLNQEE82 pKa = 4.28EE83 pKa = 4.92LGNSYY88 pKa = 8.45YY89 pKa = 10.99TNVTGTVALNEE100 pKa = 4.25YY101 pKa = 10.9FDD103 pKa = 3.89FTAFNAGQTALAQRR117 pKa = 11.84SMTLWDD123 pKa = 4.19DD124 pKa = 3.72LVAISFEE131 pKa = 4.23QTKK134 pKa = 10.63SGSADD139 pKa = 2.74ITFGNTATGGAQAYY153 pKa = 9.58AYY155 pKa = 10.36LPFGSTDD162 pKa = 3.49DD163 pKa = 4.51AEE165 pKa = 4.25ILADD169 pKa = 5.76FGFEE173 pKa = 4.23DD174 pKa = 4.19YY175 pKa = 11.3GRR177 pKa = 11.84LGGDD181 pKa = 3.02VWIDD185 pKa = 3.13GGVASNFSPLTASYY199 pKa = 10.05YY200 pKa = 10.34AQTTMVHH207 pKa = 6.7EE208 pKa = 4.89IGHH211 pKa = 7.04AIGLSHH217 pKa = 7.4PGDD220 pKa = 4.1YY221 pKa = 10.71NALDD225 pKa = 4.91DD226 pKa = 4.39NDD228 pKa = 4.33GDD230 pKa = 4.39GVPDD234 pKa = 5.79PITYY238 pKa = 10.63ASDD241 pKa = 2.91AFYY244 pKa = 10.88AQDD247 pKa = 3.19SRR249 pKa = 11.84QYY251 pKa = 11.43SIMSYY256 pKa = 10.4FDD258 pKa = 3.68AYY260 pKa = 9.1EE261 pKa = 4.12TGAQHH266 pKa = 7.31IDD268 pKa = 2.86WALLNFAYY276 pKa = 10.26AATPLVHH283 pKa = 7.61DD284 pKa = 4.55IAAIQKK290 pKa = 9.94IYY292 pKa = 10.77GVDD295 pKa = 3.03QTTRR299 pKa = 11.84TGNTVYY305 pKa = 10.74GFNATADD312 pKa = 3.52NAVYY316 pKa = 10.46DD317 pKa = 5.35FSANTRR323 pKa = 11.84PIVAIWDD330 pKa = 3.59AGGTDD335 pKa = 3.56TLDD338 pKa = 3.36FSGWNTSSTIDD349 pKa = 3.7LNQGAFSSGGGVDD362 pKa = 5.22DD363 pKa = 5.7FLTLEE368 pKa = 3.97QVNANRR374 pKa = 11.84AALGFAARR382 pKa = 11.84SQASYY387 pKa = 11.58DD388 pKa = 3.58FYY390 pKa = 11.72EE391 pKa = 4.59DD392 pKa = 4.86LKK394 pKa = 10.03TQLGLKK400 pKa = 10.19SGLFTDD406 pKa = 4.37NVSIAYY412 pKa = 9.4GAVIEE417 pKa = 4.08NAIGGGGNDD426 pKa = 3.53RR427 pKa = 11.84IIGNEE432 pKa = 3.84VANALTGGAGRR443 pKa = 11.84DD444 pKa = 3.25IFEE447 pKa = 4.73LRR449 pKa = 11.84SGGTSGADD457 pKa = 4.17RR458 pKa = 11.84ITDD461 pKa = 3.58WSRR464 pKa = 11.84GDD466 pKa = 3.43VLATTKK472 pKa = 10.69AINDD476 pKa = 3.74ANGDD480 pKa = 4.37GIITWKK486 pKa = 10.44GASLTLDD493 pKa = 3.45GSDD496 pKa = 3.53GDD498 pKa = 3.82RR499 pKa = 11.84VEE501 pKa = 5.12LVGAQGSSGLRR512 pKa = 11.84LMGSIDD518 pKa = 3.34GVFYY522 pKa = 11.46YY523 pKa = 10.62EE524 pKa = 4.33DD525 pKa = 3.38ARR527 pKa = 11.84VRR529 pKa = 11.84PVASSGQKK537 pKa = 9.65VYY539 pKa = 11.11EE540 pKa = 4.26SGFGNDD546 pKa = 3.26VLRR549 pKa = 11.84GRR551 pKa = 11.84TTASATDD558 pKa = 3.3VFFFDD563 pKa = 3.51TANPVSSLGKK573 pKa = 9.18DD574 pKa = 3.0TVSFTKK580 pKa = 10.6NDD582 pKa = 3.08IFVTTTKK589 pKa = 10.75LADD592 pKa = 3.63ANNDD596 pKa = 3.11NKK598 pKa = 9.78ITFGKK603 pKa = 10.4NGVLDD608 pKa = 4.03LGGDD612 pKa = 3.6HH613 pKa = 7.09GSVALSGTSALEE625 pKa = 3.69YY626 pKa = 11.0DD627 pKa = 4.16GVVTNNGTEE636 pKa = 4.15YY637 pKa = 10.73YY638 pKa = 10.15VYY640 pKa = 10.93SNVGSAVGVGALHH653 pKa = 6.88FF654 pKa = 4.51

Molecular weight: 69.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4GBT3|A0A0Q4GBT3_9SPHN 30S ribosomal protein S7 OS=Sphingomonas sp. Leaf20 OX=1735685 GN=rpsG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIHH32 pKa = 6.17ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3757

0

3757

1227095

26

4130

326.6

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.881 ± 0.067

0.707 ± 0.011

6.227 ± 0.035

4.665 ± 0.042

3.435 ± 0.022

9.088 ± 0.06

1.894 ± 0.021

5.081 ± 0.03

2.687 ± 0.033

9.588 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.022

2.466 ± 0.039

5.283 ± 0.034

2.977 ± 0.024

7.398 ± 0.051

5.143 ± 0.034

5.994 ± 0.042

7.537 ± 0.032

1.363 ± 0.018

2.219 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski