Hubei picorna-like virus 75

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLU5|A0A1L3KLU5_9VIRU Calici_coat domain-containing protein OS=Hubei picorna-like virus 75 OX=1923159 PE=4 SV=1
MM1 pKa = 7.6ASIAMGIGAVVSAIGTAASVGTSIANQKK29 pKa = 9.21SANSTTLEE37 pKa = 4.03NQNSVNVTNIQLQHH51 pKa = 6.36DD52 pKa = 3.72QQNFNEE58 pKa = 4.57HH59 pKa = 6.15MFDD62 pKa = 3.83KK63 pKa = 11.06NVDD66 pKa = 3.57LFTQAGLPGYY76 pKa = 8.25MAAMGGGMNNPLYY89 pKa = 10.66DD90 pKa = 3.93AFPRR94 pKa = 11.84TSQQVAGNNFSAMSMPGNVMSEE116 pKa = 3.99YY117 pKa = 10.2GGNVIQQMTGMANYY131 pKa = 9.79RR132 pKa = 11.84VSQRR136 pKa = 11.84SNINPTSTGTQTPSGSGTMNPRR158 pKa = 11.84STFSTQTEE166 pKa = 4.66SNPSGTTMYY175 pKa = 7.84ATPNTPQQLWNQLGGTTPSTSQTVAPTAAVAAPAGGSAMPMEE217 pKa = 5.63DD218 pKa = 3.96EE219 pKa = 5.21DD220 pKa = 4.95SSS222 pKa = 3.78

Molecular weight: 23.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KM10|A0A1L3KM10_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 75 OX=1923159 PE=4 SV=1
MM1 pKa = 7.36SLKK4 pKa = 10.47DD5 pKa = 3.44LRR7 pKa = 11.84IWLDD11 pKa = 3.77LIKK14 pKa = 10.74FLDD17 pKa = 3.8NNTEE21 pKa = 3.76IFKK24 pKa = 10.93DD25 pKa = 4.44GSAPEE30 pKa = 4.21RR31 pKa = 11.84PRR33 pKa = 11.84LWTLTRR39 pKa = 11.84QACFGQKK46 pKa = 8.63RR47 pKa = 11.84TWEE50 pKa = 3.94IPEE53 pKa = 3.82VCRR56 pKa = 11.84LIGCFNSTLSTYY68 pKa = 9.65IEE70 pKa = 3.92QMRR73 pKa = 11.84GIGIAIGTRR82 pKa = 11.84GVYY85 pKa = 8.33ITSPRR90 pKa = 11.84YY91 pKa = 7.85PTEE94 pKa = 3.88SDD96 pKa = 3.63LAILAALAPFSCDD109 pKa = 2.85EE110 pKa = 4.33LSKK113 pKa = 9.98HH114 pKa = 5.44TNEE117 pKa = 4.1EE118 pKa = 3.71LCEE121 pKa = 4.17FFTTLYY127 pKa = 10.9GAFEE131 pKa = 4.45NSVSIRR137 pKa = 11.84PKK139 pKa = 10.36LFKK142 pKa = 10.93LFDD145 pKa = 3.98GLRR148 pKa = 11.84HH149 pKa = 5.21FHH151 pKa = 6.79SSTSHH156 pKa = 6.06SSKK159 pKa = 10.3CANAVGKK166 pKa = 9.7FKK168 pKa = 10.65QICNCPHH175 pKa = 6.62SDD177 pKa = 3.74DD178 pKa = 4.93VIRR181 pKa = 11.84SCCSICHH188 pKa = 6.38GDD190 pKa = 3.24VHH192 pKa = 7.54SRR194 pKa = 11.84NTRR197 pKa = 11.84VYY199 pKa = 10.4SSCCSEE205 pKa = 4.21SCTSSS210 pKa = 2.81

Molecular weight: 23.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3290

210

1932

658.0

73.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.565 ± 0.602

2.036 ± 0.491

5.258 ± 0.448

5.502 ± 1.13

4.559 ± 0.233

5.897 ± 0.634

2.492 ± 0.503

5.684 ± 0.482

5.38 ± 1.184

8.298 ± 0.778

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.313 ± 0.52

4.924 ± 0.705

5.41 ± 0.628

4.134 ± 0.553

4.347 ± 0.428

7.508 ± 1.014

7.264 ± 0.638

6.565 ± 0.587

1.581 ± 0.371

3.283 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski