Ekpoma virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Tibrovirus; Ekpoma 2 tibrovirus

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5BZR0|A0A0C5BZR0_9RHAB U3 protein OS=Ekpoma virus 2 OX=1987021 PE=4 SV=1
MM1 pKa = 7.53NKK3 pKa = 9.55PDD5 pKa = 4.53FSKK8 pKa = 10.88LYY10 pKa = 10.47QNYY13 pKa = 10.34SMEE16 pKa = 4.25KK17 pKa = 10.26LRR19 pKa = 11.84DD20 pKa = 3.61SLRR23 pKa = 11.84DD24 pKa = 3.32MALQPNDD31 pKa = 4.35EE32 pKa = 4.31VPEE35 pKa = 4.33DD36 pKa = 4.84PIHH39 pKa = 6.56NNPSTSTGYY48 pKa = 8.61TNKK51 pKa = 10.08NPLEE55 pKa = 4.22SSKK58 pKa = 9.89LTPSYY63 pKa = 10.81DD64 pKa = 3.37WEE66 pKa = 4.48SSISPDD72 pKa = 3.39TSLTQQSKK80 pKa = 10.8LDD82 pKa = 4.36PIKK85 pKa = 10.48EE86 pKa = 4.23VFPMASLSDD95 pKa = 3.55ILRR98 pKa = 11.84ALALFNIHH106 pKa = 6.29EE107 pKa = 4.44SMDD110 pKa = 3.67FEE112 pKa = 4.58ISCEE116 pKa = 3.84KK117 pKa = 10.06LTFSIFPINRR127 pKa = 11.84SVTQNISSTPEE138 pKa = 3.49HH139 pKa = 6.59GKK141 pKa = 8.31TYY143 pKa = 7.13PTLRR147 pKa = 11.84EE148 pKa = 3.79VLEE151 pKa = 4.28RR152 pKa = 11.84GIIVHH157 pKa = 6.97KK158 pKa = 9.67LPSMKK163 pKa = 9.98PVTIHH168 pKa = 6.55AWTKK172 pKa = 10.52GINLEE177 pKa = 4.69KK178 pKa = 10.04IDD180 pKa = 4.32DD181 pKa = 3.99LMYY184 pKa = 10.91ADD186 pKa = 5.83DD187 pKa = 5.07MDD189 pKa = 5.21DD190 pKa = 3.79VEE192 pKa = 5.98CIAKK196 pKa = 9.15TIIKK200 pKa = 10.13ASRR203 pKa = 11.84LTRR206 pKa = 11.84SLTHH210 pKa = 6.76EE211 pKa = 4.5GAKK214 pKa = 10.14HH215 pKa = 5.42CAA217 pKa = 3.33

Molecular weight: 24.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5C384|A0A0C5C384_9RHAB Nucleocapsid protein OS=Ekpoma virus 2 OX=1987021 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 9.21KK3 pKa = 6.78TTEE6 pKa = 3.82NMGNSLSSHH15 pKa = 5.58LEE17 pKa = 3.81KK18 pKa = 10.93FKK20 pKa = 9.12TTIIWVIGGIGVCIVGCLLIKK41 pKa = 9.82CTSCLHH47 pKa = 7.45DD48 pKa = 5.86IILCCASSKK57 pKa = 9.93TSSPANKK64 pKa = 9.5QMPTIYY70 pKa = 10.37QKK72 pKa = 10.75FKK74 pKa = 8.89KK75 pKa = 9.72QRR77 pKa = 11.84RR78 pKa = 11.84KK79 pKa = 10.4KK80 pKa = 9.21MKK82 pKa = 10.63NKK84 pKa = 7.54ITKK87 pKa = 9.99LARR90 pKa = 11.84TKK92 pKa = 10.52AANKK96 pKa = 9.24TSLKK100 pKa = 10.05RR101 pKa = 11.84IKK103 pKa = 10.29PSTRR107 pKa = 11.84SSLPDD112 pKa = 3.24KK113 pKa = 10.8RR114 pKa = 11.84VGEE117 pKa = 4.39LIGKK121 pKa = 9.23YY122 pKa = 8.65EE123 pKa = 4.06AGG125 pKa = 3.53

Molecular weight: 13.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4091

125

2120

511.4

58.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.74 ± 0.295

2.151 ± 0.28

5.475 ± 0.409

5.353 ± 0.253

3.667 ± 0.378

5.353 ± 0.278

2.982 ± 0.333

8.726 ± 0.436

7.162 ± 1.035

10.364 ± 0.942

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.342

5.5 ± 0.369

5.109 ± 0.317

2.909 ± 0.181

4.351 ± 0.324

8.409 ± 0.587

5.989 ± 0.92

4.571 ± 0.247

1.76 ± 0.278

3.789 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski