Sphingobacterium gobiense

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S9JLN8|A0A2S9JLN8_9SPHI Uncharacterized protein OS=Sphingobacterium gobiense OX=1382456 GN=C5749_11240 PE=4 SV=1
MM1 pKa = 6.97YY2 pKa = 10.36RR3 pKa = 11.84SILALLLSFSFSTVFSQEE21 pKa = 3.72KK22 pKa = 10.15LFKK25 pKa = 9.7IDD27 pKa = 3.41YY28 pKa = 9.75KK29 pKa = 10.98LALSSEE35 pKa = 4.54TLTQTDD41 pKa = 3.6TTEE44 pKa = 4.73DD45 pKa = 3.35NSLDD49 pKa = 3.93LLSALAAAFGEE60 pKa = 4.45DD61 pKa = 4.4DD62 pKa = 3.98TPQVQAWVNQNFIRR76 pKa = 11.84VEE78 pKa = 4.21TQGITQNNQIQITNRR93 pKa = 11.84NTEE96 pKa = 4.22DD97 pKa = 3.63SYY99 pKa = 11.67MLYY102 pKa = 10.39PSMQAYY108 pKa = 7.44TKK110 pKa = 10.15TADD113 pKa = 3.31ATDD116 pKa = 3.27KK117 pKa = 11.41VNIHH121 pKa = 5.64EE122 pKa = 4.51TEE124 pKa = 4.13DD125 pKa = 4.75DD126 pKa = 3.48ITVTSTADD134 pKa = 3.25LPIRR138 pKa = 11.84FVADD142 pKa = 3.36TSKK145 pKa = 10.03TIAGLLCKK153 pKa = 10.25LAVLEE158 pKa = 4.28FDD160 pKa = 3.56SDD162 pKa = 3.84EE163 pKa = 4.5FNAALPQDD171 pKa = 3.53TRR173 pKa = 11.84LEE175 pKa = 3.56IWYY178 pKa = 10.03NEE180 pKa = 4.28SLPSLYY186 pKa = 9.53WGEE189 pKa = 3.8YY190 pKa = 10.27AYY192 pKa = 11.12LKK194 pKa = 9.93NIPGAALYY202 pKa = 10.1IGAFGIGIEE211 pKa = 4.27ASAIDD216 pKa = 5.22EE217 pKa = 4.26IDD219 pKa = 3.61FDD221 pKa = 4.45PSLFEE226 pKa = 4.12IPEE229 pKa = 4.55DD230 pKa = 3.56YY231 pKa = 10.39TLQEE235 pKa = 4.12NIYY238 pKa = 9.63ALSFFDD244 pKa = 4.71MPLGHH249 pKa = 6.75NLYY252 pKa = 10.49AFQDD256 pKa = 4.12STSHH260 pKa = 7.41LIGIRR265 pKa = 11.84DD266 pKa = 4.0DD267 pKa = 3.77SQQVIVEE274 pKa = 4.26PKK276 pKa = 8.2YY277 pKa = 10.61ASINEE282 pKa = 4.54FIGTRR287 pKa = 11.84AIVTNPDD294 pKa = 3.14FQYY297 pKa = 11.33GIIDD301 pKa = 3.39INGQEE306 pKa = 4.23VVPCTWEE313 pKa = 3.88YY314 pKa = 11.21LALDD318 pKa = 4.21PEE320 pKa = 4.5LEE322 pKa = 4.48IIFFSIDD329 pKa = 2.95GKK331 pKa = 9.46TGVMDD336 pKa = 3.92YY337 pKa = 10.93NRR339 pKa = 11.84EE340 pKa = 4.05TLIPAQYY347 pKa = 10.9DD348 pKa = 3.65HH349 pKa = 7.34ISFFSKK355 pKa = 10.47DD356 pKa = 3.42YY357 pKa = 11.61ALFSEE362 pKa = 4.77DD363 pKa = 4.15GKK365 pKa = 9.65TGLIDD370 pKa = 4.24LDD372 pKa = 4.09GKK374 pKa = 10.59IVLPAVFEE382 pKa = 4.67TIMEE386 pKa = 4.19YY387 pKa = 10.8DD388 pKa = 3.74EE389 pKa = 4.44SHH391 pKa = 7.04VIIIEE396 pKa = 3.82NEE398 pKa = 3.49RR399 pKa = 11.84YY400 pKa = 9.51YY401 pKa = 11.36VVDD404 pKa = 3.22IQSQQKK410 pKa = 6.22TTAGHH415 pKa = 7.41DD416 pKa = 3.7YY417 pKa = 11.26LLLANEE423 pKa = 4.39GDD425 pKa = 4.15LVVALQDD432 pKa = 3.43NKK434 pKa = 10.95YY435 pKa = 11.09GYY437 pKa = 7.88ITKK440 pKa = 9.89DD441 pKa = 3.08GKK443 pKa = 10.35IAIPFKK449 pKa = 11.04YY450 pKa = 10.04EE451 pKa = 3.76YY452 pKa = 9.0ATPFYY457 pKa = 11.11DD458 pKa = 5.78GIAAINEE465 pKa = 4.03TDD467 pKa = 4.5DD468 pKa = 6.36DD469 pKa = 4.32DD470 pKa = 4.37VLYY473 pKa = 10.54INTKK477 pKa = 9.23GEE479 pKa = 4.11YY480 pKa = 8.86VQVEE484 pKa = 4.27EE485 pKa = 4.83TEE487 pKa = 4.04

Molecular weight: 55.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S9JGL5|A0A2S9JGL5_9SPHI Diadenylate cyclase OS=Sphingobacterium gobiense OX=1382456 GN=dacA PE=3 SV=1
MM1 pKa = 7.55GFYY4 pKa = 10.28IPQKK8 pKa = 10.11FGWTIKK14 pKa = 10.29VKK16 pKa = 10.46EE17 pKa = 4.39GLVQSCSGSDD27 pKa = 3.42SARR30 pKa = 11.84LSIADD35 pKa = 3.69GVASRR40 pKa = 11.84SPYY43 pKa = 8.44QWRR46 pKa = 11.84ADD48 pKa = 3.71KK49 pKa = 11.27DD50 pKa = 3.93NAWGFEE56 pKa = 3.73RR57 pKa = 11.84RR58 pKa = 11.84EE59 pKa = 3.69RR60 pKa = 11.84HH61 pKa = 5.71HH62 pKa = 7.15NYY64 pKa = 9.95DD65 pKa = 3.44RR66 pKa = 11.84NPPQRR71 pKa = 11.84VSIVILALTSIARR84 pKa = 11.84VRR86 pKa = 11.84DD87 pKa = 3.45GLVRR91 pKa = 11.84RR92 pKa = 11.84ATT94 pKa = 3.42

Molecular weight: 10.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3791

0

3791

1386281

38

2439

365.7

41.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.198 ± 0.042

0.719 ± 0.011

5.701 ± 0.025

6.274 ± 0.034

4.804 ± 0.031

6.929 ± 0.04

2.036 ± 0.02

7.053 ± 0.035

6.207 ± 0.038

9.352 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.016

5.318 ± 0.037

3.724 ± 0.02

3.813 ± 0.022

4.671 ± 0.026

6.138 ± 0.031

5.606 ± 0.022

6.531 ± 0.024

1.298 ± 0.015

4.296 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski