Enterobacter phage phiEap-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Eapunavirus; Enterobacter virus Eap1

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FGN0|A0A0K2FGN0_9CAUD Internal virion protein D OS=Enterobacter phage phiEap-1 OX=1587520 GN=RU59_00036 PE=3 SV=1
MM1 pKa = 7.44GPSGLVGFFALGDD14 pKa = 3.77NQMSNLPFTGEE25 pKa = 3.42LDD27 pKa = 4.67AINEE31 pKa = 3.9MLAAIGEE38 pKa = 4.5APVVTLDD45 pKa = 4.53DD46 pKa = 4.21EE47 pKa = 5.09NNVDD51 pKa = 3.3VANARR56 pKa = 11.84RR57 pKa = 11.84ILNKK61 pKa = 9.68INRR64 pKa = 11.84QIQSRR69 pKa = 11.84VWTFNLEE76 pKa = 3.92EE77 pKa = 4.72GATLTPDD84 pKa = 4.57TITKK88 pKa = 9.16LIPYY92 pKa = 9.49SPDD95 pKa = 3.12YY96 pKa = 10.87LSLMNNGGASVYY108 pKa = 9.93VNRR111 pKa = 11.84QGWVYY116 pKa = 10.94DD117 pKa = 3.63KK118 pKa = 11.08SANSDD123 pKa = 3.41QFDD126 pKa = 3.77GPIAVDD132 pKa = 3.28IVRR135 pKa = 11.84LKK137 pKa = 10.69DD138 pKa = 3.54YY139 pKa = 11.62NEE141 pKa = 3.89MPEE144 pKa = 4.66CFRR147 pKa = 11.84SWIVARR153 pKa = 11.84ASRR156 pKa = 11.84QFNSRR161 pKa = 11.84FFGAEE166 pKa = 3.79DD167 pKa = 3.55VAQDD171 pKa = 4.24LAEE174 pKa = 4.42EE175 pKa = 4.22EE176 pKa = 3.53QDD178 pKa = 3.01AAMRR182 pKa = 11.84CFEE185 pKa = 4.26YY186 pKa = 10.86EE187 pKa = 3.86LDD189 pKa = 3.64YY190 pKa = 11.64GKK192 pKa = 11.22YY193 pKa = 10.93NMLDD197 pKa = 2.86GDD199 pKa = 4.31AFVGGLLSRR208 pKa = 4.64

Molecular weight: 23.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FGA0|A0A0K2FGA0_9CAUD Uncharacterized protein OS=Enterobacter phage phiEap-1 OX=1587520 GN=RU59_00012 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84LHH4 pKa = 6.24YY5 pKa = 10.28NKK7 pKa = 10.55SNGIFSVRR15 pKa = 11.84RR16 pKa = 11.84ADD18 pKa = 3.58RR19 pKa = 11.84STIAATEE26 pKa = 4.15RR27 pKa = 11.84NGKK30 pKa = 8.65LPRR33 pKa = 11.84MFDD36 pKa = 3.45TFEE39 pKa = 4.37LAPSVHH45 pKa = 6.85IIVTRR50 pKa = 11.84GLYY53 pKa = 9.07EE54 pKa = 3.98AATEE58 pKa = 4.06KK59 pKa = 10.64SRR61 pKa = 11.84PFVPVVVTKK70 pKa = 9.67WPNVRR75 pKa = 11.84GFFMRR80 pKa = 11.84LKK82 pKa = 10.45EE83 pKa = 4.46IINEE87 pKa = 4.07

Molecular weight: 10.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11942

33

1315

284.3

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.834 ± 0.532

0.821 ± 0.16

6.322 ± 0.206

6.833 ± 0.394

3.668 ± 0.151

8.131 ± 0.41

1.75 ± 0.192

5.225 ± 0.22

6.356 ± 0.317

8.131 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.671 ± 0.2

4.848 ± 0.286

3.668 ± 0.214

4.045 ± 0.322

5.133 ± 0.153

6.012 ± 0.297

5.837 ± 0.279

6.975 ± 0.317

1.449 ± 0.189

3.291 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski