Flavobacteria bacterium (strain MS024-2A)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; unclassified Flavobacteriia

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0BIU0|C0BIU0_FLABM PSP1 domain protein OS=Flavobacteria bacterium (strain MS024-2A) OX=487796 GN=Flav2ADRAFT_0752 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.37RR3 pKa = 11.84ILYY6 pKa = 9.56LFILLGFNLVYY17 pKa = 10.63AQTEE21 pKa = 4.37TFVISPGGQVSNTVIDD37 pKa = 4.5NIGQTVTTSALSGGLTLSEE56 pKa = 3.91GFLYY60 pKa = 10.93NFDD63 pKa = 3.65FTTPTVILTDD73 pKa = 3.44TDD75 pKa = 4.24SNNLVSNSDD84 pKa = 3.51VVTITATFSEE94 pKa = 4.86SMAATPTLSLSGITTGALMTASASSSIWTYY124 pKa = 7.88TWTVSTTVTSTTATVSGTDD143 pKa = 3.46LSGNAYY149 pKa = 10.31AGTDD153 pKa = 3.34SLTFSIDD160 pKa = 3.34NLGPTVVLSNNSLNNRR176 pKa = 11.84VSNTQSVTLYY186 pKa = 9.6ATFSEE191 pKa = 4.71EE192 pKa = 4.93LDD194 pKa = 3.3ITPTISLSGITTDD207 pKa = 4.0AAMSNGNTIVLKK219 pKa = 10.51PNNGWTSSSGSFQDD233 pKa = 3.97FNGSSGSHH241 pKa = 7.06PYY243 pKa = 10.01LAEE246 pKa = 4.27DD247 pKa = 3.87LVIFSYY253 pKa = 11.21LDD255 pKa = 3.1NASIYY260 pKa = 11.01KK261 pKa = 10.32NFTISSGTVSVVVKK275 pKa = 10.58LDD277 pKa = 3.48YY278 pKa = 10.63KK279 pKa = 10.78KK280 pKa = 10.86SYY282 pKa = 11.1SEE284 pKa = 3.94DD285 pKa = 3.21TGQVKK290 pKa = 10.41LYY292 pKa = 10.54YY293 pKa = 10.09YY294 pKa = 10.31DD295 pKa = 4.39ANSTLLSTDD304 pKa = 2.86ASAVLTGTSDD314 pKa = 3.6FQNLNFQSSVPSGAVVVRR332 pKa = 11.84LEE334 pKa = 4.6LIQINEE340 pKa = 4.15SEE342 pKa = 4.5FWAGNYY348 pKa = 9.23GIRR351 pKa = 11.84YY352 pKa = 8.77RR353 pKa = 11.84DD354 pKa = 3.61LEE356 pKa = 4.03ITGVDD361 pKa = 3.13EE362 pKa = 4.18GTVYY366 pKa = 10.89GGNDD370 pKa = 2.74TWSYY374 pKa = 8.14TWTVSTTATSTTATVSATDD393 pKa = 3.49NAGNSYY399 pKa = 9.43TGSEE403 pKa = 4.13SMTFVIDD410 pKa = 3.32NEE412 pKa = 4.04RR413 pKa = 11.84PYY415 pKa = 11.31AVSTTLSNNLKK426 pKa = 10.32AGYY429 pKa = 9.85ISATDD434 pKa = 3.71TNTLTITFNEE444 pKa = 4.68EE445 pKa = 3.32INASTFTASDD455 pKa = 3.53VTILPSGYY463 pKa = 10.11FSISEE468 pKa = 4.28HH469 pKa = 5.8TSSDD473 pKa = 2.92NTIFIGYY480 pKa = 9.86LNVLSNYY487 pKa = 10.07AGVVTLTLNEE497 pKa = 3.97NSLEE501 pKa = 4.17DD502 pKa = 3.65FAGNANTVASISFISDD518 pKa = 3.18YY519 pKa = 10.57TVPTVTLTDD528 pKa = 3.5TDD530 pKa = 3.56SDD532 pKa = 4.01NLVSNSEE539 pKa = 4.34VVTLTATFSEE549 pKa = 4.86SMSATPTLSLTGIVSDD565 pKa = 5.03AEE567 pKa = 4.3MTATASDD574 pKa = 4.58SIWTYY579 pKa = 7.58TWTVSGSTVTSTTATVSGTDD599 pKa = 3.54LAGNAYY605 pKa = 10.37SGTDD609 pKa = 3.42SITFTIDD616 pKa = 2.8NSNPTLVSFTDD627 pKa = 3.51SDD629 pKa = 3.58TDD631 pKa = 3.7NYY633 pKa = 9.99VNDD636 pKa = 3.91YY637 pKa = 11.04TNVTLSATFSEE648 pKa = 5.1SMASSPTIKK657 pKa = 9.5IDD659 pKa = 2.98NGTNVILVNSASMTVSSSTSSATWTYY685 pKa = 10.36FWDD688 pKa = 3.92VPGGSDD694 pKa = 3.11GTYY697 pKa = 9.37FATVSGTDD705 pKa = 3.46LSGNAYY711 pKa = 9.23TGTEE715 pKa = 4.22SITFVVDD722 pKa = 3.11NTAPLIQSVTVTNTNSKK739 pKa = 10.3VLLTYY744 pKa = 10.6NEE746 pKa = 4.23PVQLYY751 pKa = 9.66DD752 pKa = 3.43PSYY755 pKa = 8.8FASNFSVTKK764 pKa = 9.57TGGTATVSYY773 pKa = 9.41TGYY776 pKa = 10.6SFSTTDD782 pKa = 3.18SNTVILNIIVTGEE795 pKa = 3.84PTGDD799 pKa = 3.34EE800 pKa = 4.18LLEE803 pKa = 4.15VGPVGASTLIDD814 pKa = 3.33KK815 pKa = 10.6AGNYY819 pKa = 10.05AMDD822 pKa = 4.1YY823 pKa = 10.63SDD825 pKa = 5.89SNQTSNTVYY834 pKa = 9.75LTNTPPKK841 pKa = 9.67FASTSVSSNNTSITIAFSEE860 pKa = 4.93GVTADD865 pKa = 3.3TSGTEE870 pKa = 3.86LSKK873 pKa = 10.06TDD875 pKa = 3.43FTLAVSGGAAVLASATPIALSKK897 pKa = 9.63TDD899 pKa = 2.96SKK901 pKa = 10.27TYY903 pKa = 11.17VLTTSYY909 pKa = 10.93SVPSNGSEE917 pKa = 3.75ILTVTPISSAVFDD930 pKa = 4.41SKK932 pKa = 10.71GTQINLSSAQSNTVQLNDD950 pKa = 3.27QAGPFITGATLDD962 pKa = 3.67SQNRR966 pKa = 11.84YY967 pKa = 8.89VDD969 pKa = 4.04FTFSEE974 pKa = 4.79GVYY977 pKa = 9.21ATASPTSAVTSSSFTLSQSSGPSYY1001 pKa = 11.42GMTISSITTAGGGVLSGGEE1020 pKa = 3.81TTLRR1024 pKa = 11.84FNLEE1028 pKa = 4.13SAVKK1032 pKa = 8.72PTGQEE1037 pKa = 3.61VFAITATDD1045 pKa = 3.31SSSVVDD1051 pKa = 3.71ASGNSMTVSQTNNTFQLKK1069 pKa = 9.4PPTSGGVSPKK1079 pKa = 10.37KK1080 pKa = 9.62STISAAPAQMIGNGINTAVITVQAKK1105 pKa = 10.57DD1106 pKa = 3.38SLGQNFLEE1114 pKa = 4.84GGYY1117 pKa = 10.28QITLFGPNGDD1127 pKa = 3.92LTTTDD1132 pKa = 3.86NQNGTYY1138 pKa = 9.46SASYY1142 pKa = 8.54TPEE1145 pKa = 5.94AITQDD1150 pKa = 3.69FQEE1153 pKa = 4.29LTFGFRR1159 pKa = 11.84VADD1162 pKa = 3.83TNGTPTALLQLYY1174 pKa = 10.07LDD1176 pKa = 4.1EE1177 pKa = 7.41DD1178 pKa = 4.32GDD1180 pKa = 4.25GVYY1183 pKa = 10.69NINDD1187 pKa = 3.5ACLGTEE1193 pKa = 4.17AGLEE1197 pKa = 4.02VDD1199 pKa = 4.02ATGCALNQLDD1209 pKa = 4.3SDD1211 pKa = 4.09NDD1213 pKa = 4.07GVFDD1217 pKa = 6.35DD1218 pKa = 6.17LDD1220 pKa = 4.03EE1221 pKa = 5.91CPDD1224 pKa = 3.64TPEE1227 pKa = 3.94QEE1229 pKa = 4.33LNNVQGTPTYY1239 pKa = 10.93GEE1241 pKa = 5.17LIPTVVDD1248 pKa = 3.79EE1249 pKa = 4.9KK1250 pKa = 11.53GCGASQRR1257 pKa = 11.84DD1258 pKa = 3.69TDD1260 pKa = 3.45SDD1262 pKa = 4.72GIVDD1266 pKa = 4.05TEE1268 pKa = 4.71DD1269 pKa = 3.33NCIEE1273 pKa = 4.25TANADD1278 pKa = 3.69QADD1281 pKa = 3.93TDD1283 pKa = 3.96GDD1285 pKa = 4.53GIGDD1289 pKa = 3.67VCDD1292 pKa = 3.77TDD1294 pKa = 4.05NPLPEE1299 pKa = 4.07ITTTEE1304 pKa = 3.78IKK1306 pKa = 10.3FVQLPQNGTTVGKK1319 pKa = 10.34IEE1321 pKa = 4.12ATDD1324 pKa = 3.8PDD1326 pKa = 4.88GEE1328 pKa = 4.6TLTFTQSGDD1337 pKa = 3.08NFTGVLSIASDD1348 pKa = 3.71GSITVSSGALLTFDD1362 pKa = 3.47STYY1365 pKa = 11.28NGASLGFTVSDD1376 pKa = 3.93GKK1378 pKa = 10.76NDD1380 pKa = 3.21VHH1382 pKa = 8.03GSVKK1386 pKa = 10.24ILIEE1390 pKa = 4.22DD1391 pKa = 3.55VPRR1394 pKa = 11.84PPEE1397 pKa = 3.51ISIITLEE1404 pKa = 3.81ISEE1407 pKa = 4.33DD1408 pKa = 3.95AEE1410 pKa = 4.14VGTIVGFVEE1419 pKa = 5.28AKK1421 pKa = 10.67DD1422 pKa = 3.62PMGGQIVSISLQGDD1436 pKa = 3.87GFIEE1440 pKa = 4.59LKK1442 pKa = 10.97DD1443 pKa = 3.82GVLKK1447 pKa = 8.13TTQEE1451 pKa = 3.63LDD1453 pKa = 3.47YY1454 pKa = 10.88EE1455 pKa = 4.66EE1456 pKa = 4.66NTIHH1460 pKa = 7.41PFTITAQASDD1470 pKa = 3.75RR1471 pKa = 11.84SDD1473 pKa = 3.72GPGLTGSKK1481 pKa = 10.13SEE1483 pKa = 4.18SLRR1486 pKa = 11.84VSDD1489 pKa = 5.16IPNATYY1495 pKa = 9.69TGRR1498 pKa = 11.84FFISIFNVEE1507 pKa = 4.71DD1508 pKa = 3.28EE1509 pKa = 4.46TLGAKK1514 pKa = 9.38VDD1516 pKa = 3.17HH1517 pKa = 6.38RR1518 pKa = 11.84RR1519 pKa = 11.84YY1520 pKa = 9.97FNPYY1524 pKa = 8.43NKK1526 pKa = 9.82NVGKK1530 pKa = 9.69WKK1532 pKa = 9.64IKK1534 pKa = 10.18KK1535 pKa = 9.34RR1536 pKa = 11.84VAGGADD1542 pKa = 3.28ADD1544 pKa = 3.84KK1545 pKa = 9.91FTIKK1549 pKa = 9.5TRR1551 pKa = 11.84SKK1553 pKa = 9.78TEE1555 pKa = 3.69QKK1557 pKa = 10.74NDD1559 pKa = 3.98DD1560 pKa = 4.6PIEE1563 pKa = 4.62DD1564 pKa = 3.95EE1565 pKa = 4.56NEE1567 pKa = 4.13DD1568 pKa = 3.73YY1569 pKa = 11.43LDD1571 pKa = 5.53FITPPDD1577 pKa = 3.73YY1578 pKa = 11.24EE1579 pKa = 4.46NPGDD1583 pKa = 3.97ANGDD1587 pKa = 3.53NIYY1590 pKa = 10.04EE1591 pKa = 4.23VEE1593 pKa = 4.09VEE1595 pKa = 4.49YY1596 pKa = 11.62VNTDD1600 pKa = 3.06DD1601 pKa = 5.26GAPEE1605 pKa = 3.94VPIVVTQTNIQVPEE1619 pKa = 4.36GSTTTIEE1626 pKa = 4.29LQSQPVLPTDD1636 pKa = 4.88DD1637 pKa = 4.22NDD1639 pKa = 4.08GDD1641 pKa = 4.34GVPDD1645 pKa = 5.11IIDD1648 pKa = 3.84NSPLVANADD1657 pKa = 3.62QSDD1660 pKa = 3.7EE1661 pKa = 4.92DD1662 pKa = 4.55GDD1664 pKa = 4.48GVGDD1668 pKa = 3.68VSDD1671 pKa = 5.72DD1672 pKa = 3.75FDD1674 pKa = 6.61HH1675 pKa = 7.6DD1676 pKa = 4.74GVWNPFDD1683 pKa = 3.8TCPDD1687 pKa = 3.35TSLGEE1692 pKa = 4.1IVDD1695 pKa = 4.2SNGCLIYY1702 pKa = 9.64YY1703 pKa = 9.69IPSSNFSISKK1713 pKa = 8.28TEE1715 pKa = 3.66KK1716 pKa = 10.19CAGTNSINIEE1726 pKa = 4.16VVDD1729 pKa = 3.66TSITYY1734 pKa = 9.4NVSVSGAATLSDD1746 pKa = 4.05SFSSSSWSLDD1756 pKa = 3.2NLSAGVYY1763 pKa = 8.61SICITVEE1770 pKa = 4.28GVSSAEE1776 pKa = 3.78FEE1778 pKa = 4.28RR1779 pKa = 11.84CFEE1782 pKa = 4.24VTISEE1787 pKa = 4.44PDD1789 pKa = 3.67PLIVNSMFNKK1799 pKa = 9.88NDD1801 pKa = 3.19QTITFGLNGGVSYY1814 pKa = 10.65DD1815 pKa = 3.2ITHH1818 pKa = 6.75NGITTQTTKK1827 pKa = 10.74SSYY1830 pKa = 9.45TVSLAKK1836 pKa = 10.16GLNRR1840 pKa = 11.84ISISTGIEE1848 pKa = 3.95CQGLFEE1854 pKa = 4.1NTYY1857 pKa = 10.03LNSYY1861 pKa = 6.82EE1862 pKa = 4.25VKK1864 pKa = 10.17YY1865 pKa = 10.88APNPFSSEE1873 pKa = 3.81LTFTIGGEE1881 pKa = 3.88DD1882 pKa = 2.92RR1883 pKa = 11.84VFGLEE1888 pKa = 3.96VYY1890 pKa = 9.37TPSGQLIDD1898 pKa = 3.72QKK1900 pKa = 11.4VITLPFGIRR1909 pKa = 11.84YY1910 pKa = 7.34YY1911 pKa = 10.5TLQTDD1916 pKa = 3.63NYY1918 pKa = 8.57KK1919 pKa = 10.45QGGYY1923 pKa = 9.96FIRR1926 pKa = 11.84VKK1928 pKa = 10.78GSTIDD1933 pKa = 3.71QSFQVIKK1940 pKa = 10.3EE1941 pKa = 3.98

Molecular weight: 205.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0BKI2|C0BKI2_FLABM Histidine triad (HIT) protein OS=Flavobacteria bacterium (strain MS024-2A) OX=487796 GN=Flav2ADRAFT_0055 PE=4 SV=1
MM1 pKa = 7.02VVVAHH6 pKa = 6.61LVRR9 pKa = 11.84APDD12 pKa = 3.98FGTGDD17 pKa = 4.72LISSRR22 pKa = 11.84VLKK25 pKa = 10.95NSFPFNRR32 pKa = 11.84FTNIFSS38 pKa = 3.58

Molecular weight: 4.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1770

0

1770

592508

32

5671

334.8

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.538 ± 0.064

0.744 ± 0.021

5.35 ± 0.095

6.492 ± 0.063

5.287 ± 0.051

6.78 ± 0.057

1.874 ± 0.034

7.842 ± 0.049

7.149 ± 0.095

9.762 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.037

5.574 ± 0.063

3.76 ± 0.031

3.697 ± 0.041

3.559 ± 0.046

6.968 ± 0.063

5.601 ± 0.112

5.998 ± 0.047

1.159 ± 0.023

3.678 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski