Algibacter lectus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group;

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090VNL6|A0A090VNL6_9FLAO Uncharacterized protein OS=Algibacter lectus OX=221126 GN=JCM19300_8 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.43FKK4 pKa = 10.97LFNPLYY10 pKa = 10.57LFTLTLFVASCSSDD24 pKa = 3.79DD25 pKa = 4.48DD26 pKa = 5.15GGDD29 pKa = 3.19TSEE32 pKa = 5.1SIEE35 pKa = 3.93KK36 pKa = 10.51SAVIEE41 pKa = 4.17NYY43 pKa = 10.98ANLVYY48 pKa = 10.42QSYY51 pKa = 10.75LDD53 pKa = 4.03SYY55 pKa = 9.24TGAVSLQTAVDD66 pKa = 3.8VFIEE70 pKa = 4.52TPTDD74 pKa = 3.48ANFTAVKK81 pKa = 8.58TAWLSARR88 pKa = 11.84DD89 pKa = 3.55IYY91 pKa = 11.0GQTEE95 pKa = 4.59AYY97 pKa = 9.73RR98 pKa = 11.84EE99 pKa = 4.25CNGPIDD105 pKa = 4.23TEE107 pKa = 4.15NEE109 pKa = 3.55AWSVGNEE116 pKa = 4.05GQLNAWPIDD125 pKa = 3.58EE126 pKa = 5.38SYY128 pKa = 11.03IDD130 pKa = 3.85YY131 pKa = 11.01VAFGTEE137 pKa = 4.14AYY139 pKa = 9.8AGSFTSIISNTSVTIDD155 pKa = 3.47EE156 pKa = 4.56ATLASLNEE164 pKa = 3.94EE165 pKa = 4.35TTDD168 pKa = 3.92KK169 pKa = 11.12SISTGWHH176 pKa = 6.0AIEE179 pKa = 4.24FLLWGQDD186 pKa = 2.97NTLPSEE192 pKa = 4.41DD193 pKa = 4.38LPGQRR198 pKa = 11.84EE199 pKa = 4.15YY200 pKa = 11.29TDD202 pKa = 3.22YY203 pKa = 8.58TTADD207 pKa = 3.66DD208 pKa = 4.01ADD210 pKa = 3.71RR211 pKa = 11.84RR212 pKa = 11.84ADD214 pKa = 3.72YY215 pKa = 10.72LQAATDD221 pKa = 4.28LLVSDD226 pKa = 5.61LNALTTTWAPNGTYY240 pKa = 8.81RR241 pKa = 11.84TVFEE245 pKa = 5.1ALDD248 pKa = 3.51EE249 pKa = 4.46DD250 pKa = 4.25VALKK254 pKa = 10.47QFINGAFFIAGDD266 pKa = 3.86EE267 pKa = 4.2LSSEE271 pKa = 4.5RR272 pKa = 11.84IIAPVDD278 pKa = 3.49STDD281 pKa = 4.4GIDD284 pKa = 4.43GLGQEE289 pKa = 5.39DD290 pKa = 4.13EE291 pKa = 4.83HH292 pKa = 9.27SCFSDD297 pKa = 3.36NTHH300 pKa = 6.57KK301 pKa = 10.9DD302 pKa = 2.46IWANAQGVYY311 pKa = 10.54NVVFGAYY318 pKa = 9.96GNVSGDD324 pKa = 3.17SFYY327 pKa = 11.68DD328 pKa = 3.51LVKK331 pKa = 10.98EE332 pKa = 3.92KK333 pKa = 10.89DD334 pKa = 3.05AAQAEE339 pKa = 4.48KK340 pKa = 10.85LKK342 pKa = 10.99DD343 pKa = 3.5AADD346 pKa = 3.55EE347 pKa = 4.19AMEE350 pKa = 4.36KK351 pKa = 10.73VNAIANNSEE360 pKa = 3.88PFDD363 pKa = 3.86YY364 pKa = 11.12LITLEE369 pKa = 4.55NSTDD373 pKa = 3.39ANFGVVMQSVVALQDD388 pKa = 3.11WADD391 pKa = 4.41EE392 pKa = 4.27ISASATVIGINLL404 pKa = 3.48

Molecular weight: 44.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090WUR1|A0A090WUR1_9FLAO Glycosyltransferase OS=Algibacter lectus OX=221126 GN=JCM19274_1496 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.66LSVSTEE48 pKa = 3.92TRR50 pKa = 11.84HH51 pKa = 6.44KK52 pKa = 10.57KK53 pKa = 9.81

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4609

0

4609

1356275

37

3966

294.3

33.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.396 ± 0.035

0.742 ± 0.014

5.849 ± 0.033

6.487 ± 0.032

5.217 ± 0.029

6.34 ± 0.038

1.746 ± 0.019

7.803 ± 0.039

7.732 ± 0.052

9.128 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.112 ± 0.018

6.561 ± 0.042

3.303 ± 0.023

3.182 ± 0.02

3.169 ± 0.021

6.646 ± 0.033

6.097 ± 0.042

6.298 ± 0.031

1.089 ± 0.013

4.105 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski